NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1638 [new locus tag: SAUSA300_RS08935 ]
• pan locus tag^?: SAUPAN004316000
• symbol: phoR
• pan gene symbol^?: phoR
• synonym:
• product: sensory box histidine kinase PhoR

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1638 [new locus tag: SAUSA300_RS08935 ]
• symbol: phoR
• product: sensory box histidine kinase PhoR
• replicon: chromosome
• strand: -
• coordinates: 1795536..1797197
• length: 1662
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912917 NCBI
• RefSeq: YP_494333 NCBI
• MicrobesOnline: 1293153 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAGTTTCACCACCGCTTAATGTTATTGATAAGCAGTATATTAATTATCAGTTTTGTT
  ACATTAGGCATTATTATTCACGCTTCCATCACATATGTTTTATCAGAAAAACACGAACAA
  GCTCTAGCAACAGATGCAAGATCATATATTTATTTAGTCCAAGATAATGAGATTGATAAA
  GTTAAAAAAATTGTAAAACAGCAAAATATAGATTTATTGATAACTAAGCATGATAAAAAG
  GTGTTTAGTAGTGGCAAACTGAAAGCTATCCCAAATAGTGATAGCTATATTCAAAAAAGG
  GACGTTTTTATTTTTAATAAGAAGGTTAACGGTTATCATGTATGGATTAAAGGTTACAAT
  AACGATATTACAGAAATGCATTGGACGTTGTGGAAATATCTTATTTTGACATGTTTAGTT
  GTACTCATTTGTCTGTATTTTGCAAGCCGTAGTTTTAAGCGTACATTAATCCGTCCCATT
  CAAGAAGTTACTTATGCAACACAATTGTTGGCAAATGGTTATTATCACATACGTGTACCT
  GAAAGTAATGTTGTAGAAACTAAAGCGCTTTTTGTGTCTACCAATGATTTAGCACGACGC
  CTTCAAAAGCTTAATAATGAGCAAAAAATTCAATCCAACCGTTTAAAAACAACGATTGAA
  AATATTCCAAGTGCTATTTTAATGATAGATCGAAATGGAAAAATCGTTGTTGCAAATAAA
  GCGTATTATGAACAGTTCAACATCTCGCATAATATTGAACAAGTAGGCTATCACGGCTAT
  GTTAATACTGAAATTGAGCAATTAATTTTAGAAAGTTTTAAAGTTGAAAAACCTATTTAT
  GAACAATTGGAAGTTGCAATAAACCAAGTCCATGCCAAGTACTTTGATATTTCATGCGTT
  CCGATTTTAACTAGGTCTCAAAAAAGTTTGCAAGGGATTTTAGTTGTGATGCATGATATA
  ACAAATCTGAAACAACTTGAAAATCTAAGACGTGAATTTGTAGCAAATGTTTCACATGAA
  TTAAAAACACCTATTACTTCAATTAAGGGGTTTGCTGAAACGCTTATTGATGGTGCAAAA
  AATGATGCGGAATCATTAGACATGTTTTTAAACATAATTTTAAAAGAATCAAATCGAATA
  GAATCTTTAGTAACAGATCTGTTAGATTTATCGCATATTGAGCAACATACAGAGTTAGAT
  ACAGACTATATGAATTTATCAGATTTAACGCGGCGTATTATTGATAATATGATGACTCAA
  GCCAATCAAAAAAATATTTCCATTCATACTGATATTGAAAAAGATGTCATTGTTAAAGCA
  CAAGAAAGTAAAATTGCTCAAGTTATCACGAATTTGTTAACGAATGCAATTAACTATTCT
  TATGAAGATGGAGATATTAACGTTCGTGTGTATCGAGATGACTTTCGTGTCATTTTCGAA
  GTACAAGATTTTGGTATAGGTATTAAATTAGAAGACCAACAACGTATTTTTGAAAGATTT
  TATAGAGTAGATAAAGCACGAAGTAGAGATTCTGGTGGGACAGGTCTTGGATTATCAATT
  ACAAAGCATATAGTAGAGGCCCACCAAGGCAATATTGAAGTGAATAGTCAAGTTGGCAAA
  GGCTCAACGTTCAAAGTTATTCTAAAAGATTATAAAGAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1638 [new locus tag: SAUSA300_RS08935 ]
• symbol: PhoR
• description: sensory box histidine kinase PhoR
• length: 553
• theoretical pI: 8.11061
• theoretical MW: 63772.9
• GRAVY: -0.20217

⊟Function

• reaction:
  EC 2.7.3.-?  ExPASy
  ATP + protein L-histidine = ADP + protein N-phospho-L-histidine?
• TIGRFAM:
  Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 307.5)
  Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 149.9)
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 141.8)
  Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
  Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 70.8)
  MetabolismCentral intermediary metabolismNitrogen fixationnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
  Signal transductionRegulatory functionsProtein interactionsnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
  Signal transductionRegulatory functionsSmall molecule interactionsPAS domain S-box protein (TIGR00229; HMM-score: 27.8)
  MetabolismEnergy metabolismPhotosynthesistranscriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
  Signal transductionRegulatory functionsDNA interactionstranscriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
• TheSEED:  
  Phosphorus MetabolismPhosphorus Metabolism - no subcategoryHigh affinity phosphate transporter and control of PHO regulon Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3) 
  Phosphorus Metabolism - no subcategoryPhosphate metabolism Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3) 
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 91)
  HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 70.5)
  PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 27.1)
  PAS_4; PAS fold (PF08448; HMM-score: 25)
  PAS_7; PAS fold (PF12860; HMM-score: 22.5)
  His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 20.4)
  PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 18.6)
  His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 18.4)
  PAS_Fold (CL0183) PAS_8; PAS domain (PF13188; HMM-score: 17.7)
  no clan definedSecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 14.1)
  MKT1_N; Temperature dependent protein affecting M2 dsRNA replication (PF12247; HMM-score: 12.2)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.241
  • Ymax_pos: 29
  • Cmax: 0.222
  • Cmax_pos: 44
  • Smax: 0.594
  • Smax_pos: 1
  • Smean: 0.371
  • D: 0.292
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 87160006 NCBI
• RefSeq: YP_494333 NCBI
• UniProt: A0A0H2XFS9 UniProt

⊟Protein sequence

• MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALATDARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDVFIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPIQEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTTIENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVEKPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFVANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSHIEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVITNLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARSRDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: phoR < phoP

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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