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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1801 [new locus tag: SACOL_RS09230 ]
  • pan locus tag?: SAUPAN004429000
  • symbol: SACOL1801
  • pan gene symbol?:
  • synonym:
  • product: dipeptidase PepV

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1801 [new locus tag: SACOL_RS09230 ]
  • symbol: SACOL1801
  • product: dipeptidase PepV
  • replicon: chromosome
  • strand: -
  • coordinates: 1848684..1850093
  • length: 1410
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTGGAAAGAAAAAGTTCAACAATACGAAGATCAAATCATTAATGACTTAAAAGGATTA
    TTAGCAATTGAAAGTGTGAGAGATGATGCAAAAGCATCAGAAGACGCACCAGTTGGTCCA
    GGTCCTCGTAAGGCATTAGACTACATGTATGAAATTGCACATAGAGATGGATTTACAACA
    CATGATGTGGATCATATTGCAGGAAGAATTGAGGCAGGTAAAGGAAATGACGTATTAGGT
    ATCTTATGTCATGTTGACGTTGTTCCTGCTGGTGATGGATGGGATAGTAATCCGTTCGAG
    CCGGTTGTAACAGAAGATGCTATCATAGCTAGAGGTACACTTGATGACAAAGGTCCAACA
    ATTGCTGCTTATTATGCAATTAAGATATTAGAAGATATGAATGTGGATTGGAAGAAACGT
    ATTCATATGATTATTGGTACGGATGAAGAATCTGATTGGAAATGTACGGATCGCTATTTT
    AAAACAGAAGAAATGCCAACATTAGGTTTTGCACCAGATGCAGAATTTCCATGTATTCAT
    GGTGAAAAAGGCATTACAACATTTGATTTAGTTCAAAATAAACTTACTGAAGATCAAGAT
    GAACCTGATTATGAATTAATAACTTTTAAATCTGGTGAACGTTACAACATGGTACCTGAT
    CATGCAGAAGCAAGAGTGCTTGTTAAAGAAAATATGACAGATGTTATTCAAGACTTTGAG
    TACTTTTTAGAACAAAATCATTTACAAGGTGATAGTACTGTTGATAGTGGCATTCTAGTT
    TTAACAGTTGAAGGTAAAGCGGTTCATGGTATGGATCCATCTATCGGTGTGAATGCGGGT
    CTTTACTTACTAAAATTCTTAGCATCATTAAATCTTGATAATAATGCACAAGCGTTTGTA
    GCATTTAGTAATCGCTACTTATTTAATTCAGATTTTGGTGAAAAGATGGGAATGAAATTC
    CATACAGATGTCATGGGTGACGTGACAACTAACATTGGTGTTATTACATATGATAATGAA
    AACGCAGGTCTTTTCGGTATCAACTTACGCTACCCAGAAGGATTTGAATTTGAAAAAGCT
    ATGGATCGTTTTGCAAATGAGATTCAACAATATGGCTTTGAAGTGAAATTAGGTAAAGTC
    CAACCACCACATTATGTTGATAAAAATGATCCTTTTGTACAAAAGTTAGTTACTGCATAT
    AGAAATCAAACAAATGATATGACTGAACCTTATACTATAGGTGGCGGTACTTATGCGAGA
    AACTTAGACAAGGGTGTAGCATTTGGCGCAATGTTTAGTGATTCTGAAGATTTAATGCAT
    CAGAAAAATGAATATATCACTAAAAAACAGTTATTTAACGCAACTAGTATTTACTTAGAA
    GCAATTTATTCATTATGCGTGGAGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1410

Protein[edit | edit source]

Protein Data Bank: 3KHX
Protein Data Bank: 3KHZ
Protein Data Bank: 3KI9

General[edit | edit source]

  • locus tag: SACOL1801 [new locus tag: SACOL_RS09230 ]
  • symbol: SACOL1801
  • description: dipeptidase PepV
  • length: 469
  • theoretical pI: 4.32275
  • theoretical MW: 52823.9
  • GRAVY: -0.408316

Function[edit | edit source]

  • reaction:
    EC 3.4.13.-?  ExPASy
  • TIGRFAM:
    putative dipeptidase (TIGR01887; EC 3.4.13.-; HMM-score: 637.7)
    and 11 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides dipeptidase PepV (TIGR01886; EC 3.4.13.-; HMM-score: 459.3)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 105.3)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 62.3)
    putative selenium metabolism hydrolase (TIGR03526; HMM-score: 45)
    N-acyl-L-amino-acid amidohydrolase (TIGR01880; EC 3.5.1.14; HMM-score: 43.4)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 40.5)
    peptidase T-like protein (TIGR01883; HMM-score: 30.6)
    N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 30.6)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 19.6)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 14.9)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 11.8)
  • TheSEED  :
    • Putative Xaa-His dipeptidase
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 97.4)
    and 2 more
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 32.4)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 13)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014046
    • TAT(Tat/SPI): 0.001237
    • LIPO(Sec/SPII): 0.001764
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRKALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPVVTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYFKTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMVPDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIGVNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGVITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPFVQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQLFNATSIYLEAIYSLCVEE

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 2708 [5]
  • interaction partners:
    SACOL0452(ahpC)alkyl hydroperoxide reductase subunit C  [6] (data from MRSA252)
    SACOL2657(arcA)arginine deiminase  [6] (data from MRSA252)
    SACOL1800(dat)D-alanine aminotransferase  [6] (data from MRSA252)
    SACOL0461(guaA)GMP synthase  [6] (data from MRSA252)
    SACOL1011(ppnK)inorganic polyphosphate/ATP-NAD kinase  [6] (data from MRSA252)
    SACOL2233(rpsC)30S ribosomal protein S3  [6] (data from MRSA252)
    SACOL10315' nucleotidase  [6] (data from MRSA252)

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: 31.52 h [7]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. 6.0 6.1 6.2 6.3 6.4 6.5 6.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  7. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]

H De Lencastre, S W Wu, M G Pinho, A M Ludovice, S Filipe, S Gardete, R Sobral, S Gill, M Chung, A Tomasz
Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin.
Microb Drug Resist: 1999, 5(3);163-75
[PubMed:10566865] [WorldCat.org] [DOI] (P p)
Tavarekere S Girish, Balasubramanian Gopal
Crystal structure of Staphylococcus aureus metallopeptidase (Sapep) reveals large domain motions between the manganese-bound and apo-states.
J Biol Chem: 2010, 285(38);29406-15
[PubMed:20610394] [WorldCat.org] [DOI] (I p)