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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09230 [old locus tag: SACOL1801 ]
  • pan locus tag?: SAUPAN004429000
  • symbol: SACOL_RS09230
  • pan gene symbol?:
  • synonym:
  • product: dipeptidase PepV

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS09230 [old locus tag: SACOL1801 ]
  • symbol: SACOL_RS09230
  • product: dipeptidase PepV
  • replicon: chromosome
  • strand: -
  • coordinates: 1848684..1850093
  • length: 1410
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1848684..1850093) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTGGAAAGAAAAAGTTCAACAATACGAAGATCAAATCATTAATGACTTAAAAGGATTA
    TTAGCAATTGAAAGTGTGAGAGATGATGCAAAAGCATCAGAAGACGCACCAGTTGGTCCA
    GGTCCTCGTAAGGCATTAGACTACATGTATGAAATTGCACATAGAGATGGATTTACAACA
    CATGATGTGGATCATATTGCAGGAAGAATTGAGGCAGGTAAAGGAAATGACGTATTAGGT
    ATCTTATGTCATGTTGACGTTGTTCCTGCTGGTGATGGATGGGATAGTAATCCGTTCGAG
    CCGGTTGTAACAGAAGATGCTATCATAGCTAGAGGTACACTTGATGACAAAGGTCCAACA
    ATTGCTGCTTATTATGCAATTAAGATATTAGAAGATATGAATGTGGATTGGAAGAAACGT
    ATTCATATGATTATTGGTACGGATGAAGAATCTGATTGGAAATGTACGGATCGCTATTTT
    AAAACAGAAGAAATGCCAACATTAGGTTTTGCACCAGATGCAGAATTTCCATGTATTCAT
    GGTGAAAAAGGCATTACAACATTTGATTTAGTTCAAAATAAACTTACTGAAGATCAAGAT
    GAACCTGATTATGAATTAATAACTTTTAAATCTGGTGAACGTTACAACATGGTACCTGAT
    CATGCAGAAGCAAGAGTGCTTGTTAAAGAAAATATGACAGATGTTATTCAAGACTTTGAG
    TACTTTTTAGAACAAAATCATTTACAAGGTGATAGTACTGTTGATAGTGGCATTCTAGTT
    TTAACAGTTGAAGGTAAAGCGGTTCATGGTATGGATCCATCTATCGGTGTGAATGCGGGT
    CTTTACTTACTAAAATTCTTAGCATCATTAAATCTTGATAATAATGCACAAGCGTTTGTA
    GCATTTAGTAATCGCTACTTATTTAATTCAGATTTTGGTGAAAAGATGGGAATGAAATTC
    CATACAGATGTCATGGGTGACGTGACAACTAACATTGGTGTTATTACATATGATAATGAA
    AACGCAGGTCTTTTCGGTATCAACTTACGCTACCCAGAAGGATTTGAATTTGAAAAAGCT
    ATGGATCGTTTTGCAAATGAGATTCAACAATATGGCTTTGAAGTGAAATTAGGTAAAGTC
    CAACCACCACATTATGTTGATAAAAATGATCCTTTTGTACAAAAGTTAGTTACTGCATAT
    AGAAATCAAACAAATGATATGACTGAACCTTATACTATAGGTGGCGGTACTTATGCGAGA
    AACTTAGACAAGGGTGTAGCATTTGGCGCAATGTTTAGTGATTCTGAAGATTTAATGCAT
    CAGAAAAATGAATATATCACTAAAAAACAGTTATTTAACGCAACTAGTATTTACTTAGAA
    GCAATTTATTCATTATGCGTGGAGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1410

Protein[edit source | edit]

Protein Data Bank: 3KHX
Protein Data Bank: 3KHZ
Protein Data Bank: 3KI9

General[edit source | edit]

  • locus tag: SACOL_RS09230 [old locus tag: SACOL1801 ]
  • symbol: SACOL_RS09230
  • description: dipeptidase PepV
  • length: 469
  • theoretical pI: 4.32275
  • theoretical MW: 52823.9
  • GRAVY: -0.408316

Function[edit source | edit]

  • reaction:
    EC 3.4.13.-?  ExPASy
  • TIGRFAM:
    putative dipeptidase (TIGR01887; EC 3.4.13.-; HMM-score: 637.7)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesdipeptidase PepV (TIGR01886; EC 3.4.13.-; HMM-score: 459.3)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 105.3)
    MetabolismAmino acid biosynthesisAspartate familysuccinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 62.3)
    putative selenium metabolism hydrolase (TIGR03526; HMM-score: 45)
    N-acyl-L-amino-acid amidohydrolase (TIGR01880; EC 3.5.1.14; HMM-score: 43.4)
    MetabolismAmino acid biosynthesisGlutamate familyacetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 40.5)
    peptidase T-like protein (TIGR01883; HMM-score: 30.6)
    N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 30.6)
    MetabolismAmino acid biosynthesisAspartate familysuccinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 19.6)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 14.9)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesamidohydrolase (TIGR01891; HMM-score: 11.8)
  • TheSEED:
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 97.4)
    no clan definedM20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 32.4)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.162
    • Ymax_pos: 33
    • Cmax: 0.28
    • Cmax_pos: 33
    • Smax: 0.164
    • Smax_pos: 30
    • Smean: 0.072
    • D: 0.127
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446187562 NCBI
  • RefSeq: WP_000265417 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRKALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPVVTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYFKTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMVPDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIGVNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGVITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPFVQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQLFNATSIYLEAIYSLCVEE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS09230 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]