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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1943 [new locus tag: SACOL_RS10160 ]
  • pan locus tag?: SAUPAN004900000
  • symbol: vraS
  • pan gene symbol?: vraS
  • synonym:
  • product: sensor histidine kinase VraS

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1943 [new locus tag: SACOL_RS10160 ]
  • symbol: vraS
  • product: sensor histidine kinase VraS
  • replicon: chromosome
  • strand: -
  • coordinates: 2005560..2006603
  • length: 1044
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAACCACTACATTAGAACAATTGGTTCAATGCTCATCTTAGTATATAGCATGCTAGCT
    GCATTTCTGTTCATCGATAAAGTTTTTGTAAATATCATCTATTTTCAAGGTATGTTTTAT
    ACACAAATATTCGGAATACCAGTTTTTTTATTTTTAAATCTCATCATCATATTATTGTGT
    ATTATTGTTGGTTCGGTACTCGCTTACAAAATCAATCAGCAAAATGATTGGATTAAGACG
    CAAATTGAGCGTTCAATGGAAGGCGAAACAGTTGGCATTAATGATCAAAATATAGAAATA
    TATAGTGAAACGTTAGATTTATACCATACACTCGTACCTTTAAATCAAGAATTGCATAAG
    TTGCGACTTAAAACTCAAAACTTAACCAATGAAAATTATAATATTAATGATGTGAAAGTT
    AAAAAGATTATTGAAGATGAACGTCAAAGACTAGCACGAGAACTTCACGATTCTGTTAGT
    CAGCAACTTTTTGCGGCAAGTATGATGCTATCTGCTATCAAAGAAACGAAGTTAGAACCA
    CCATTAGACCAACAAATTCCTATTTTAGAGAAAATGGTTCAAGATTCGCAGTTAGAAATG
    CGTGCTTTGCTGTTACATTTAAGACCGCTTGGTTTAAAAGACAAATCTTTAGGTGAGGGT
    ATTAAAGATTTAGTTATTGATTTACAAAAAAAAGTGCCAATGAAAGTTGTGCATGAAATA
    CAAGATTTTAAAGTGCCTAAAGGTATTGAAGATCATTTGTTCAGAATTACACAGGAAGCA
    ATTTCGAATACATTGCGTCATTCAAACGGTACAAAAGTGACAGTAGAATTGTTTAATAAA
    GACGATTATTTATTGTTGAGAATTCAAGATAATGGTAAAGGTTTTAATGTTGATGAAAAA
    TTAGAACAAAGTTATGGACTTAAAAATATGCGTGAAAGAGCTTTGGAAATTGGTGCAACG
    TTCCATATTGTATCATTGCCAGATTCAGGTACACGTATCGAGGTGAAAGCACCTTTAAAT
    AAGGAGGATTCGTATGACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1943 [new locus tag: SACOL_RS10160 ]
  • symbol: VraS
  • description: sensor histidine kinase VraS
  • length: 347
  • theoretical pI: 6.06404
  • theoretical MW: 40044.2
  • GRAVY: -0.145533

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.5)
  • TheSEED:  
    CBSS-176280.1.peg.1561 Sensor histidine kinase VraS 
  • PFAM:
    His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 60.1)
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 33.2)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 17.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.134
    • Ymax_pos: 12
    • Cmax: 0.119
    • Cmax_pos: 69
    • Smax: 0.233
    • Smax_pos: 7
    • Smean: 0.185
    • D: 0.154
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIFGIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYSETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVSQQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLGEGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVELFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIEVKAPLNKEDSYDD

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

S Gardete, S W Wu, S Gill, A Tomasz
Role of VraSR in antibiotic resistance and antibiotic-induced stress response in Staphylococcus aureus.
Antimicrob. Agents Chemother.: 2006, 50(10);3424-34
[PubMed:17005825] [WorldCat.org] [DOI] (P p)
Benjamin P Howden, Danielle J Smith, Ashley Mansell, Paul D R Johnson, Peter B Ward, Timothy P Stinear, John K Davies
Different bacterial gene expression patterns and attenuated host immune responses are associated with the evolution of low-level vancomycin resistance during persistent methicillin-resistant Staphylococcus aureus bacteraemia.
BMC Microbiol.: 2008, 8;39
[PubMed:18304359] [WorldCat.org] [DOI] (I e)
Elena Galbusera, Adriana Renzoni, Diego O Andrey, Antoinette Monod, Christine Barras, Paolo Tortora, Alessandra Polissi, William L Kelley
Site-specific mutation of Staphylococcus aureus VraS reveals a crucial role for the VraR-VraS sensor in the emergence of glycopeptide resistance.
Antimicrob. Agents Chemother.: 2011, 55(3);1008-20
[PubMed:21173175] [WorldCat.org] [DOI] (I p)
Susana Gardete, Choonkeun Kim, Boris M Hartmann, Michael Mwangi, Christelle M Roux, Paul M Dunman, Henry F Chambers, Alexander Tomasz
Genetic pathway in acquisition and loss of vancomycin resistance in a methicillin resistant Staphylococcus aureus (MRSA) strain of clonal type USA300.
PLoS Pathog.: 2012, 8(2);e1002505
[PubMed:22319446] [WorldCat.org] [DOI] (I p)