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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1984 [new locus tag: SACOL_RS10370 ]
  • pan locus tag?: SAUPAN004956000
  • symbol: aldA2
  • pan gene symbol?: aldH
  • synonym:
  • product: aldehyde dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1984 [new locus tag: SACOL_RS10370 ]
  • symbol: aldA2
  • product: aldehyde dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2046906..2048285
  • length: 1380
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATATCATTGAGCAAAAATTTTATGACAGTAAAGCTTTTTTCAATACACAACAAACT
    AAAGATATTAGTTTTAGAAAAGAGCAATTAAAGAAGTTAAGCAAAGCTATTAAATCATAC
    GAGAGCGATATTTTAGAAGCACTATATACAGATTTAGGAAAAAATAAAGTCGAAGCTTAT
    GCTACTGAAATTGGCATAACTTTGAAAAGTATCAAAATTGCCCGTAAGGAACTTAAAAAC
    TGGACTAAAACAAAAAATGTAGACACACCTTTATATTTATTTCCAACAAAAAGCTATATC
    AAAAAAGAACCTTATGGAACAGTTTTGATCATTGCACCATTTAACTATCCTTTTCAACTA
    GTGTTCGAACCTTTAATCGGTGCTATTGCAGCAGGTAATACGGCAATTATTAAACCATCT
    GAGTTGACGCCTAATGTTGCACGAGTGATTAAACGGTTAATCAATGAAACATTTGATGCA
    AATTACATTGAAGTTATTGAGGGAGGAATTGAAGAAACGCAAACGTTAATTCACTTACCT
    TTTGACTATGTCTTCTTTACAGGAAGTGAAAATGTAGGCAAAATCGTTTATCAAGCTGCC
    AGCGAAAATTTAGTTCCTGTGACATTAGAAATGGGCGGTAAATCTCCAGTCATCGTTGAT
    GAAACAGCGAATATTAAAGTTGCTAGTGAGCGCATTTGTTTTGGGAAATTCACTAATGCC
    GGCCAAACATGTGTTGCACCAGATTACATTTTAGTACACGAATCTGTAAAAGATGATTTA
    ATCACAGCCCTATCAAAAACGTTGCGTGAATTTTATGGTCAAAATATACAACAAAGTCCA
    GATTATGGCCGCATTGTAAACCTTAAACATTATCATCGTCTGACTTCATTACTTAACAGT
    GCACAAATGAATATTGTATTTGGTGGTCATAGTGATGAGGATGAACGTTATATAGAACCA
    ACATTGTTAGATCACGTTACAAGTGATTCAGCAATTATGCAAGAAGAAATTTTTGGTCCT
    ATCTTACCGATTTTAACGTATCAGTCATTGGATGAAGCAATAGCCTTTATTCACCAAAGA
    CCAAAACCTTTGAGTTTATATTTATTTAGCGAAGATGAAAATGCTACACAACGTGTAATA
    AACGAGCTATCATTTGGCGGCGGCGCTATTAATGATACATTGATGCACCTAGCGAATCCT
    AAATTACCATTTGGTGGTGTTGGCGCCTCAGGTATGGGACGCTATCATGGTAAATATTCA
    TTCGACACTTTTACACATGAAAAAAGCTACATTTTCAAATCTACACGATTAGAATCAGGT
    GTCCATTTACCACCATATAAAGGTAAATTTAAATACATCAAAGCTTTCTTTAAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1984 [new locus tag: SACOL_RS10370 ]
  • symbol: AldA2
  • description: aldehyde dehydrogenase
  • length: 459
  • theoretical pI: 7.12638
  • theoretical MW: 51740.9
  • GRAVY: -0.218736

Function[edit source | edit]

  • reaction:
    EC 1.2.1.3?  ExPASy
    Aldehyde dehydrogenase (NAD+)An aldehyde + NAD+ + H2O = a carboxylate + NADH
  • TIGRFAM:
    MetabolismCentral intermediary metabolismOthersuccinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 209.5)
    Cellular processesCellular processesAdaptations to atypical conditionsbetaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 199.4)
    Unknown functionEnzymes of unknown specificityaldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 188.6)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 182.3)
    MetabolismEnergy metabolismOther2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 168.3)
    MetabolismEnergy metabolismAmino acids and aminessuccinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 157.6)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 154.5)
    MetabolismEnergy metabolismAmino acids and aminesputative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 146.1)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 146)
    MetabolismEnergy metabolismAmino acids and aminesmethylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 122.2)
    MetabolismEnergy metabolismAmino acids and aminesdelta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 99.3)
    MetabolismEnergy metabolismAmino acids and amines1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 91.8)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 71.3)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 45.5)
    MetabolismAmino acid biosynthesisGlutamate familyglutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 25.1)
    acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 20.8)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismMethylglyoxal Metabolism Aldehyde dehydrogenase (EC 1.2.1.3) 
    Central carbohydrate metabolismPyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate Aldehyde dehydrogenase (EC 1.2.1.3) 
    Fatty Acids, Lipids, and IsoprenoidsPhospholipidsGlycerolipid and Glycerophospholipid Metabolism in Bacteria Aldehyde dehydrogenase (EC 1.2.1.3) 
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 320.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL1494(asnC)asparaginyl-tRNA synthetase  [1] (data from MRSA252)
    SACOL1016(fabI)enoyl-ACP reductase  [1] (data from MRSA252)
    SACOL1513(hup)DNA-binding protein HU  [1] (data from MRSA252)
    SACOL1741(icd)isocitrate dehydrogenase  [1] (data from MRSA252)
    SACOL2213(rpoA)DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)
    SACOL1753universal stress protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.105
    • Ymax_pos: 60
    • Cmax: 0.12
    • Cmax_pos: 60
    • Smax: 0.136
    • Smax_pos: 2
    • Smean: 0.09
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDILEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKKEPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDANYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVIVDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNIQQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQEEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAINDTLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKFKYIKAFFKN

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [2] [3]
    quantitative data / protein copy number per cell: 187 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • aldA2 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]