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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2107 [new locus tag: SACOL_RS11025 ]
  • pan locus tag?: SAUPAN005406000
  • symbol: SACOL2107
  • pan gene symbol?: ptpB
  • synonym:
  • product: phosphotyrosine protein phosphatase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2107 [new locus tag: SACOL_RS11025 ]
  • symbol: SACOL2107
  • product: phosphotyrosine protein phosphatase
  • replicon: chromosome
  • strand: -
  • coordinates: 2167486..2167905
  • length: 420
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAAGATTTTATTCGTTTGTACAGGTAACACATGTCGTAGCCCATTAGCGGAAAGTATT
    GCAAAAGAGGTTATGCCAAATCATCAATTTGAATCAAGAGGTATATTCGCTGTGAACAAT
    CAAGGTGTTTCGAATTATGTTGAAGACTTAGTTGAAGAACATCATTTAGCTGAAACGACC
    TTATCGCAACAATTTACTGAAGCAGATTTGAAAGCAGATATTATTTTGACGATGTCGTAT
    TCGCACAAAGAATTAATAGAGGCACACTTTGGTTTGCAAAATCATGTTTTCACATTGCAT
    GAATATGTAAAAGAAGCAGGAGAAGTTATAGATCCATACGGTGGAACAAAAGAAATGTAT
    GTACATACCTATGAAGAACTTGTAAGTTTAATTTTAAAATTAAAAGATATTATTTGCTAG
    60
    120
    180
    240
    300
    360
    420

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2107 [new locus tag: SACOL_RS11025 ]
  • symbol: SACOL2107
  • description: phosphotyrosine protein phosphatase
  • length: 139
  • theoretical pI: 4.81841
  • theoretical MW: 15787.9
  • GRAVY: -0.109353

Function[edit source | edit]

  • reaction:
    EC 3.1.3.48?  ExPASy
    Protein-tyrosine-phosphataseProtein tyrosine phosphate + H2O = protein tyrosine + phosphate
  • TIGRFAM:
    Cellular processesCellular processesDetoxificationarsenate reductase (thioredoxin) (TIGR02691; EC 1.20.4.-,3.1.3.48; HMM-score: 28.3)
    Cellular processesCellular processesDetoxificationarsenate reductase, glutathione/glutaredoxin type (TIGR02689; EC 1.20.4.1; HMM-score: 14.6)
  • TheSEED:  
    CBSS-176280.1.peg.1561 Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) 
    LMPTP YfkJ cluster Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) 
    LMPTP YwlE cluster Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) 
  • PFAM:
    Phosphatase (CL0031) LMWPc; Low molecular weight phosphotyrosine protein phosphatase (PF01451; HMM-score: 82.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.279
    • Ymax_pos: 22
    • Cmax: 0.17
    • Cmax_pos: 22
    • Smax: 0.575
    • Smax_pos: 16
    • Smean: 0.448
    • D: 0.345
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVSNYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEYVKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 54 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]