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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2146 [new locus tag: SACOL_RS11235 ]
  • pan locus tag?: SAUPAN005495000
  • symbol: SACOL2146
  • pan gene symbol?: mtlF
  • synonym:
  • product: PTS system, mannitol-specific IIBC components

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2146 [new locus tag: SACOL_RS11235 ]
  • symbol: SACOL2146
  • product: PTS system, mannitol-specific IIBC components
  • replicon: chromosome
  • strand: +
  • coordinates: 2208813..2210351
  • length: 1539
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCACAAACTGAAGAGAAAAAAGGAATTGGTCGTCGTGTTCAAGCATTTGGATCATTC
    TTAAGTAGTATGATCATGCCAAACATTGGCGCTTTCATCGCTTGGGGTTTTATCGCGGCC
    ATTTTTATAGATAATGGATGGTTACCTAACAAAGATTTAGCTACTTTAGCAGGACCAATG
    ATTACTTATTTAATCCCATTATTGATCGCATTTAGTGGTGGTCGTTTAATTTATGATTTA
    CGTGGTGGTATCATCGCAGCAACAGCAACTATGGGGGTCATCGTTGCATTACCTGATACA
    CCAATGTTACTTGGTGCAATGATTATGGGTCCACTTGTTGGTTGGTTAATGAAGAAGACC
    GACCAATTGATTCAACCTAGAACGCCGCAAGGTTTTGAAATGTTATTTAATAACTTTTCT
    GCTGGTATTTTAGGATTTATCATGACGATTGCAGGATTTAAAATTTTAGCACCACTTATG
    AAATTTATTATGCATATTTTATCAGTGGCAGTTGAAGCATTAGTACATGCACACCTACTT
    CCACTTGTAAGTATTTTAGTAGAGCCTGCAAAAATTGTATTTTTAAACAATGCGATTAAC
    CATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTA
    TACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTT
    GGAAAAGGCACTGCAAAAACAACGTCATATGGTGCTGGAATTATCCACTTCTTAGGTGGT
    ATTCATGAAATTTATTTCCCATATGTATTAATGCGTCCTTTATTATTTATCGCTGTTATT
    TTAGGTGGAATGACTGGTGTTGCAACTTATCAAGCAACAGGATTTGGATTTAAAAGTCCC
    GCGTCACCAGGTTCATTTATAGTTTACTGTTTAAATGCACCTAGAGGTGAATTCTTGCAC
    ATGTTACTCGGTGTCTTCCTTGCTGCACTTGTATCATTCGTTGTAGCTGCTTTAATTATG
    AAGTTCACTAGAGAACCAAAGCAGGATTTAGAAGCTGCGACAGCTCAAATGGAAAATACT
    AAAGGGAAAAAATCAAGCGTTGCTTCTAAGTTAGTATCTTCTGATAAAAATGTTAATACA
    GAAGAAAATGCTAGTGGTAATGTTAGTGAAACATCTTCATCAGATGATGATCCTGAAGCG
    CTATTGGATAATTACAACACTGAAGATGTTGATGCACACAATTACAATAATATAAATCAT
    GTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGT
    AATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAA
    TTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAA
    CAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGATATGAA
    GAACTTTTAAATAATCTAAAAAAAGATGATCAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1539

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2146 [new locus tag: SACOL_RS11235 ]
  • symbol: SACOL2146
  • description: PTS system, mannitol-specific IIBC components
  • length: 512
  • theoretical pI: 7.76606
  • theoretical MW: 55095
  • GRAVY: 0.294336

Function[edit source | edit]

  • reaction:
    EC 2.7.1.197?  ExPASy
    Protein-N(pi)-phosphohistidine--D-mannitol phosphotransferase[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, mannitol-specific IIC component (TIGR00851; EC 2.7.1.69; HMM-score: 588.7)
    Signal transductionSignal transductionPTSPTS system, mannitol-specific IIC component (TIGR00851; EC 2.7.1.69; HMM-score: 588.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 14.9)
    Signal transductionSignal transductionPTSPTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 14.9)
  • TheSEED:  
    Mannitol Utilization PTS system, mannitol-specific IIB component (EC 2.7.1.69) / PTS system, mannitol-specific IIC component (EC 2.7.1.69) 
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 59.7)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 44)
    Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 16.3)
    no clan definedCtr; Ctr copper transporter family (PF04145; HMM-score: 13.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.229
    • Ymax_pos: 34
    • Cmax: 0.291
    • Cmax_pos: 34
    • Smax: 0.273
    • Smax_pos: 2
    • Smean: 0.167
    • D: 0.205
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIFGKGTAKTTSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKKDDQA

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SACOL2147 (MtlR*)
    MtlR* (TF) important in Mannitol utilizationRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]