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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2178 [new locus tag: SACOL_RS11460 ]
  • pan locus tag?: SAUPAN005583000
  • symbol: SACOL2178
  • pan gene symbol?:
  • synonym:
  • product: alcohol dehydrogenase, zinc-containing

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2178 [new locus tag: SACOL_RS11460 ]
  • symbol: SACOL2178
  • product: alcohol dehydrogenase, zinc-containing
  • replicon: chromosome
  • strand: -
  • coordinates: 2260085..2261086
  • length: 1002
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAAAATAAACAAATTCTTTTTAACAAAATACCAGAAGGCATGCCTCAAGAGGATACA
    TTCAAAATTGAAGAGATTGACACGCCTAAACTTGAGAGCGATGGTGTACTTGTACAAACG
    CTGTATATTTCAGTGGATCCATATATGAGAGGACGTATGACTAAGGCAGATTCGTATGTA
    CAGCCTTTTGAAATTGGTAAACCGATTGTTAGCCATGTCGTTGCAAAAGTAATTGATTCG
    ACACTCGCAGATTATAAAAAAGGAGATGTCGTTGTAGGTATGTTGCCTTGGCGAATCATT
    AATCATGTCCAAGCGGATCAAATTACTAAAGTTCCAACGACAGATGTACCACTTGATTTA
    TATTTAAGTGTTTTAGGAATGCCAGGACAAACAGCATATCATGGTCTGCTTGATATTGGA
    CAACCTAAAGCCGGTGATACTGTTGTGGTTTCAGCTGCATCTGGTGCTGTAGGTTCAGTT
    GTAGGTCAAATTGCTAAGATTAAAGGTTGCCGTGTAGTAGGTATTGCGGGTGGCGACAAA
    AAAGTAAATTATTTAACAGAGACACTGGGCTTTGACGCTGGTATTGATTATAAGAAAGAG
    GATTTTGCAGAAGCGTTAGCACAAGCAGTACCAGATGGTATTGATGTTTATTTTGAAAAT
    GTTGGTGGAACAGTTGGCGATGAAGTATTTAAGCATTTAAATCGTTTTGCACGTGTACCA
    GTATGTGGCGCAATTTCGTCATATAATCATCCTGAAGCCGATATCGGACCACGTATTCAA
    GGTACGCTTATTAAGAAGCAAGTAATGATGAGAGGCTTCTTGGTAAGTGAATTCGCTAAT
    GCTTTTAAAGAAGCTAGTGAACAGCTTGCAACATGGGTTCAAGAAGGTAAAATTCAGTCA
    CAAGTAACCATTGAAGATGGTTTTGAAAATGCGCCACATGCATTTAAAAATTTATTTACT
    GGCGATAACTTTGGTAAACAAGTTATTAAAGTAACGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2178 [new locus tag: SACOL_RS11460 ]
  • symbol: SACOL2178
  • description: alcohol dehydrogenase, zinc-containing
  • length: 333
  • theoretical pI: 5.08506
  • theoretical MW: 36266.2
  • GRAVY: -0.0948949

Function[edit source | edit]

  • reaction:
    EC 1.1.1.1?  ExPASy
    Alcohol dehydrogenaseAn alcohol + NAD+ = an aldehyde or ketone + NADHA secondary alcohol + NAD+ = a ketone + NADH
  • TIGRFAM:
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 267.3)
    Unknown functionEnzymes of unknown specificityputative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 86.4)
    Unknown functionEnzymes of unknown specificityputative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 44.5)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 34.2)
    MetabolismEnergy metabolismAmino acids and aminesL-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 22.6)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 13.4)
    MetabolismEnergy metabolismFermentationzinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 12.7)
  • TheSEED:  
    MiscellaneousMiscellaneous - no subcategoryBroadly distributed proteins not in subsystems Putative oxidoreductase YncB 
  • PFAM:
    GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 117.7)
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 72.5)
    ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 52.5)
    no clan definedCas_VVA1548; Putative CRISPR-associated protein (Cas_VVA1548) (PF09652; HMM-score: 14.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.105
    • Ymax_pos: 23
    • Cmax: 0.121
    • Cmax_pos: 53
    • Smax: 0.138
    • Smax_pos: 22
    • Smean: 0.1
    • D: 0.103
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYISVDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINHVQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSVVGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYFENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVSEFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 758 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]