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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02447
  • pan locus tag?: SAUPAN005583000
  • symbol: SAOUHSC_02447
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02447
  • symbol: SAOUHSC_02447
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2270736..2271737
  • length: 1002
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAAAATAAACAAATTCTTTTTAACAAAATACCAGAAGGCATGCCTCAAGAGGATACA
    TTCAAAATTGAAGAGATTGACACGCCTAAACTTGAGAGCGATGGTGTACTTGTACAAACG
    CTGTATATTTCAGTGGATCCATATATGAGAGGACGTATGACTAAGGCAGATTCGTATGTA
    CAGCCTTTTGAAATTGGTAAACCGATTGTTAGCCATGTCGTTGCAAAAGTAATTGATTCG
    ACACTCGCAGATTATAAAAAAGGAGATGTCGTTGTAGGTATGTTGCCTTGGCGAATCATT
    AATCATGTCCAAGCGGATCAAATTACTAAAGTTCCAACGACAGATGTACCACTTGATTTA
    TATTTAAGTGTTTTAGGAATGCCAGGACAAACAGCATATCATGGTCTGCTTGATATTGGA
    CAACCTAAAGCCGGTGATACTGTTGTGGTTTCAGCTGCATCTGGTGCTGTAGGTTCAGTT
    GTAGGTCAAATTGCTAAGATTAAAGGTTGCCGTGTAGTAGGTATTGCGGGTGGCGACAAA
    AAAGTAAATTATTTAACAGAGACACTGGGCTTTGACGCTGGTATTGATTATAAGAAAGAG
    GATTTTGCAGAAGCGTTAGCACAAGCAGTACCAGATGGTATTGATGTTTATTTTGAAAAT
    GTTGGTGGAACAGTTGGCGATGAAGTATTTAAGCATTTAAATCGTTTTGCACGTGTACCA
    GTATGTGGCGCAATTTCGTCATATAATCATCCTGAAGCCGATATCGGACCACGTATTCAA
    GGTACGCTTATTAAGAAGCAAGTAATGATGAGAGGCTTCTTGGTAAGTGAATTCGCTAAT
    GCTTTTAAAGAAGCTAGTGAACAGCTTGCAACATGGGTTCAAGAAGGTAAAATTCAGTCA
    CAAGTAACCATTGAAGATGGTTTTGAAAATGCGCCACATGCATTTAAAAATTTATTTACT
    GGCGATAACTTTGGTAAACAAGTTATTAAAGTAACGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02447
  • symbol: SAOUHSC_02447
  • description: hypothetical protein
  • length: 333
  • theoretical pI: 5.08506
  • theoretical MW: 36266.2
  • GRAVY: -0.0948949

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 267.3)
    and 6 more
    Unknown functionEnzymes of unknown specificityputative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 86.4)
    Unknown functionEnzymes of unknown specificityputative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 44.5)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 34.2)
    MetabolismEnergy metabolismAmino acids and aminesL-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 22.6)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 13.4)
    MetabolismEnergy metabolismFermentationzinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 12.7)
  • TheSEED:  
    Broadly distributed proteins not in subsystems NADP-dependent oxidoreductase YfmJ 
  • PFAM:
    GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 117.7)
    and 3 more
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 72.5)
    ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 52.5)
    no clan definedCas_VVA1548; Putative CRISPR-associated protein (Cas_VVA1548) (PF09652; HMM-score: 14.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.105
    • Ymax_pos: 23
    • Cmax: 0.121
    • Cmax_pos: 53
    • Smax: 0.138
    • Smax_pos: 22
    • Smean: 0.1
    • D: 0.103
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYISVDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINHVQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSVVGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYFENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVSEFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]