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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2364 [new locus tag: SACOL_RS12415 ]
  • pan locus tag?: SAUPAN005880000
  • symbol: SACOL2364
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2364 [new locus tag: SACOL_RS12415 ]
  • symbol: SACOL2364
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2424261..2425934
  • length: 1674
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATTAAACAGATAAATGTTTCTAATATGCAAAAGTTTGAATCTCAATTAATGAAAGCT
    CAGTCTGAAGGTTATACACATGTCGTTCCATATGCCAATGAGATAATGATTTATCAATCG
    ATGTTAGATGCGGTTCAATTATATCCTAAGTCTATCGTGGTTGATTATACTGTGGATGGA
    CAATACAAAAATGATTGTCACTATTTCGGTCAATCATCGATCAACATTGCCGATTGGGCT
    CAAAACAATAATTATTATCCTAACCTGATATATGCGATTCAACAGACTCTTGATTTAATA
    CATTATTACTCTGTAGAAACGATTTTCGATTTAGCTTTATTGACACTTCTGAAAGGTGAT
    TTATCAATCGATGGTCATGTCGTTTTTGATTTTAAAGCCCCTATAGCAACAAGTGCTTCA
    ATATGGGAAACTATTAAAACTATTGAAGATTTTGATATGATGTCTCAGTTTTATTTGAAT
    AAAATGGCTTATATTGATCATCATCCTATACCTTTTCGCAACCTTTTTATCGAGGATTCA
    GAACAACTTAATTCGCCAGATAATTGGTTGTATTCTACCAAGTTTATGCTACCTAAATGG
    TTATATAAAATAGCAAAGCAACGTGCTGATAACAAACAATTACAAAACTTCGGCCTGTAT
    ACGAAGCAACCAAACGTTTTAAAAGATCACATCGTTTTTATCGGTGATCATCATCAATAT
    ATAGGTAATTCAAAATATTTATTTACTTACTTCGTTAAACATAATCCAATGACTGCCTGT
    TATTTTGTTACTGACGATCGTCGCGGACCACATTTCATATCACCTAAGTCTGAAAAAGCT
    GACGAATTGATTAACAGCGCACGCGTAGTTATAGTTGAAAATGATATACCTGAAACGTTA
    CAACCAAACGGTACTTTAATTCAATTACATCAAGGCACACCAATAATGCAATTATTTTTA
    GATAGTAAAGAGCCTATTAAAAATATAGAAACACCTTTTTATCGTGCTAAAAGGTATAAT
    CGCTGGTTGCAATTCGATTATGTTATACATTCTGCTGATGATATTAGCCACTTTTATCAA
    ACGGCTTTTCCTAGTCACCAAGCAAATGTATTGGCTTATGGTAATCCTAAACACCAATAT
    TTACTACAAAAGAGTAACGAAAGTACTACTCAACAACAGTATAAGAAATCGTTTAAAATA
    AATGATCAAAAACCAGTTTTATTATACGCACCTATTGGATTAGTGAGTGCGCAACAATTA
    CCACTATCAGATGCTTTATTCAAAGCCTATCATGTTGTGGTTCAGGGTGTTGATGAAGCG
    ATCTTACCAGAAGAGGCGCTTGTTGCACCAAAGTATCTTAGTGCTCAAGACTTAATTCTA
    ATGTCTGATGTCGTTATCACTGATTACTCAAATATAATATTTGATGCGATGGCTATAGAC
    AAAACAGTAGCATTATACACTCCAAATCACAGTCAATACATTGAGTCTCAAGGTGTTAAC
    GAAGATATTTGGCGTCATCTATCAAAAATTTGGTATACAGACCGACAATTATTAATTAAT
    AATTTAATTTCCCAGGCAATACCAGTGATTAAATATCCACAAATACAACATAAAGAACAA
    CCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1674

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2364 [new locus tag: SACOL_RS12415 ]
  • symbol: SACOL2364
  • description: hypothetical protein
  • length: 557
  • theoretical pI: 6.34443
  • theoretical MW: 64536.1
  • GRAVY: -0.29228

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 16.8)
  • TheSEED:  
    Cell Wall and CapsuleGram-Positive cell wall componentsTeichoic and lipoteichoic acids biosynthesis Putative glycosyl/glycerophosphate transferase in teichoic acid biosynthesis 
  • PFAM:
    GT-B (CL0113) Glyphos_transf; CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase (PF04464; HMM-score: 98.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.178
    • Ymax_pos: 23
    • Cmax: 0.14
    • Cmax_pos: 23
    • Smax: 0.326
    • Smax_pos: 8
    • Smean: 0.222
    • D: 0.195
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDAVQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHYYSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLNKMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFGLYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPKSEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFYRAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQQYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAPKYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLSKIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL2364 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]