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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02649
  • pan locus tag?: SAUPAN005880000
  • symbol: SAOUHSC_02649
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02649
  • symbol: SAOUHSC_02649
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2434925..2436598
  • length: 1674
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATTAAACAGATAAATGTTTCTAATATGCAAAAGTTTGAATCTCAATTAATGAAAGCT
    CAGTCTGAAGGTTATACACATGTCGTTCCATATGCCAATGAGATAATGATTTATCAATCG
    ATGTTAGATGCGGTTCAATTATATCCTAAGTCTATCGTGGTTGATTATACTGTGGATGGA
    CAATACAAAAATGATTGTCACTATTTCGGTCAATCATCGATCAACATTGCCGATTGGGCT
    CAAAACAATAATTATTATCCTAACCTGATATATGCGATTCAACAGACTCTTGATTTAATA
    CATTATTACTCTGTAGAAACGATTTTCGATTTAGCTTTATTGACACTTCTGAAAGGTGAT
    TTATCAATCGATGGTCATGTCGTTTTTGATTTTAAAGCCCCTATAGCAACAAGTGCTTCA
    ATATGGGAAACTATTAAAACTATTGAAGATTTTGATATGATGTCTCAGTTTTATTTGAAT
    AAAATGGCTTATATTGATCATCATCCTATACCTTTTCGCAACCTTTTTATCGAGGATTCA
    GAACAACTTAATTCGCCAGATAATTGGTTGTATTCTACCAAGTTTATGCTACCTAAATGG
    TTATATAAAATAGCAAAGCAACGTGCTGATAACAAACAATTACAAAACTTCGGCCTGTAT
    ACGAAGCAACCAAACGTTTTAAAAGATCACATCGTTTTTATCGGTGATCATCATCAATAT
    ATAGGTAATTCAAAATATTTATTTACTTACTTCGTTAAACATAATCCAATGACTGCCTGT
    TATTTTGTTACTGACGATCGTCGCGGACCACATTTCATATCACCTAAGTCTGAAAAAGCT
    GACGAATTGATTAACAGCGCACGCGTAGTTATAGTTGAAAATGATATACCTGAAACGTTA
    CAACCAAACGGTACTTTAATTCAATTACATCAAGGCACACCAATAATGCAATTATTTTTA
    GATAGTAAAGAGCCTATTAAAAATATAGAAACACCTTTTTATCGTGCTAAAAGGTATAAT
    CGCTGGTTGCAATTCGATTATGTTATACATTCTGCTGATGATATTAGCCACTTTTATCAA
    ACGGCTTTTCCTAGTCACCAAGCAAATGTATTGGCTTATGGTAATCCTAAACACCAATAT
    TTACTACAAAAGAGTAACGAAAGTACTACTCAACAACAGTATAAGAAATCGTTTAAAATA
    AATGATCAAAAACCAGTTTTATTATACGCACCTATTGGATTAGTGAGTGCGCAACAATTA
    CCACTATCAGATGCTTTATTCAAAGCCTATCATGTTGTGGTTCAGGGTGTTGATGAAGCG
    ATCTTACCAGAAGAGGCGCTTGTTGCACCAAAGTATCTTAGTGCTCAAGACTTAATTCTA
    ATGTCTGATGTCGTTATCACTGATTACTCAAATATAATATTTGATGCGATGGCTATAGAC
    AAAACAGTAGCATTATACACTCCAAATCACAGTCAATACATTGAGTCTCAAGGTGTTAAC
    GAAGATATTTGGCGTCATCTATCAAAAATTTGGTATACAGACCGACAATTATTAATTAAT
    AATTTAATTTCCCAGGCAATACCAGTGATTAAATATCCACAAATACAACATAAAGAACAA
    CCATTAGAATCTATTTCACAACTTATATTGTCTAAGATGACATCTAATCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1674

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02649
  • symbol: SAOUHSC_02649
  • description: hypothetical protein
  • length: 557
  • theoretical pI: 6.34443
  • theoretical MW: 64536.1
  • GRAVY: -0.29228

Function[edit source | edit]

  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 16.8)
  • TheSEED:  
    Cell Wall and CapsuleGram-Positive cell wall componentsTeichoic and lipoteichoic acids biosynthesis Putative glycosyl/glycerophosphate transferase in teichoic acid biosynthesis 
  • PFAM:
    GT-B (CL0113) Glyphos_transf; CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase (PF04464; HMM-score: 98.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.178
    • Ymax_pos: 23
    • Cmax: 0.14
    • Cmax_pos: 23
    • Smax: 0.326
    • Smax_pos: 8
    • Smean: 0.222
    • D: 0.195
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDAVQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHYYSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLNKMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFGLYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPKSEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFYRAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQQYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAPKYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLSKIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_02649 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]