From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2435 [new locus tag: SACOL_RS12780 ]
  • pan locus tag?: SAUPAN005980000
  • symbol: SACOL2435
  • pan gene symbol?:
  • synonym:
  • product: glycerate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2435 [new locus tag: SACOL_RS12780 ]
  • symbol: SACOL2435
  • product: glycerate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 2492841..2493983
  • length: 1143
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAATTACAAAAAATTGTCATCGCTCCTGACTCATTTAAGGAAAGTATGACCGCACAG
    CAAGTTGGCAATATTATAAAACAGGCTTTTACTAATGTTTATGGGAATACCCTTCATTAT
    GATATCATTCCGATGGCTGATGGTGGTGAAGGTACCACAGATGCTTTAATGCATGCAACA
    GGTGCCACTAAGTATACAGTCATCGTTAATGACCCTTTAATGCGACCTATTGAAGCATGT
    TATGCACGCGCAGACGAACAACAAATTGCAATTATTGAAATGGCGGCAGCGTCAGGTTTG
    GATTTATTAGAAAAAGAGGAACGTAATCCTTTATACACATCATCATATGGTACCGGTGAA
    CTAATTAAAGATGCATTAAATCATGGTGCTAAGACCATTATTTTAGGGATTGGTGGCAGT
    GCAACAAATGATGGTGGTACAGGTATGCTAAGTGCACTAGGCGTAAAGTTTACTGATGTA
    AACGGGGACTTATTACAAATGAATGGTGCTAATCTTGCTCACATTGCACAAATCGATATA
    ACCAATCTAGATTCGCGATTAAAAGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAAT
    CCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATGCA
    AAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGC
    ACAGGAAAGTCCGTTAATCAAATACCAGGTTCTGGTGCAGCTGGCGGTATGGGCGCAGCA
    TTATTAGCGTTTTGTGAGACAACTTTAACAAAAGGTATTGATGTCGTCTTTGACATTACA
    GATTTTCATCAAAGAATTAAAGATGCAGACCTCGTTATTACTGGAGAAGGACGCATGGAT
    TATCAGACCATCTTTGGTAAAACACCCGTAGGCGTTGCGTTAGCTGCAAAACAATATCAT
    ATTCCTGTCATCGCGATTTGTGGCAGTCTAGGCGAAAATTATCAACATGTTTACGATTTC
    GGTATTGATAGTGCCTATTCTATAATCTCTTCACCTAGCACTTTAGAAGATGTCCTACAA
    AATAGCGAACAAAATTTATTAAACACTGCAACTGACATTGCTCGTATTCTGAAATTACAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2435 [new locus tag: SACOL_RS12780 ]
  • symbol: SACOL2435
  • description: glycerate kinase
  • length: 380
  • theoretical pI: 4.76396
  • theoretical MW: 40660.1
  • GRAVY: -0.02

Function[edit source | edit]

  • reaction:
    EC 2.7.1.31?  ExPASy
    Glycerate 3-kinaseATP + D-glycerate = ADP + 3-phospho-D-glycerate
  • TIGRFAM:
    MetabolismEnergy metabolismOtherglycerate kinase (TIGR00045; EC 2.7.1.31; HMM-score: 576.3)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization Glycerate kinase (EC 2.7.1.31) 
    CarbohydratesMonosaccharidesD-galactarate, D-glucarate and D-glycerate catabolism - gjo Glycerate kinase (EC 2.7.1.31) 
    MonosaccharidesD-galactarate, D-glucarate and D-glycerate catabolism Glycerate kinase (EC 2.7.1.31) 
    One-carbon MetabolismSerine-glyoxylate cycle Glycerate kinase (EC 2.7.1.31) 
    Organic acidsGlycerate metabolism Glycerate kinase (EC 2.7.1.31) 
    Fatty Acids, Lipids, and IsoprenoidsPhospholipidsGlycerolipid and Glycerophospholipid Metabolism in Bacteria Glycerate kinase (EC 2.7.1.31) 
  • PFAM:
    no clan definedGly_kinase; Glycerate kinase family (PF02595; HMM-score: 484.1)
    NADP_Rossmann (CL0063) Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 11.6)
    no clan definedT6SS_VipA; Type VI secretion system, VipA, VC_A0107 or Hcp2 (PF05591; HMM-score: 11.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.146
    • Ymax_pos: 11
    • Cmax: 0.123
    • Cmax_pos: 25
    • Smax: 0.257
    • Smax_pos: 3
    • Smean: 0.227
    • D: 0.177
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIPMADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDLLEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDVNGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGADAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVVFDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENYQHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]