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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2451 [new locus tag: SACOL_RS12865 ]
  • pan locus tag?: SAUPAN005998000
  • symbol: SACOL2451
  • pan gene symbol?: opuCC
  • synonym:
  • product: amino acid ABC transporter amino acid-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2451 [new locus tag: SACOL_RS12865 ]
  • symbol: SACOL2451
  • product: amino acid ABC transporter amino acid-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2510752..2511693
  • length: 942
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAGAAAATTAAATATATACTTGTCGTGTTTGTCTTATCGCTTACCGTATTATCTGGA
    TGTAGTTTGCCCGGACTAGGTAGTAAGAGCACGAAAAATGATGTCAAAATTACAGCATTA
    TCAACAAGCGAATCGCAAATTATTTCACATATGTTACGGTTGTTAATAGAGCATGATACA
    CACGGTAAGATAAAGCCAACATTAGTAAATAATTTAGGGTCAAGTACGATTCAACATAAT
    GCCTTAATTAATGGGGATGCTAATATATCAGGTGTTAGATATAATGGCACAGATTTAACG
    GGAGCTTTGAAGGAAGCACCAATTAAAAATCCTAAGAAAGCAATGATAGCAACACAACAA
    GGATTTAAAAAGAAATTTGATCAAACGTTTTTTGATTCGTATGGTTTTGCGAATACGTAT
    GCATTCATGGTAACGAAGGAAACCGCTAAAAAATATCATTTAGAGACAGTTTCAGATTTA
    GCAAAGCATAGTAAAGATTTACGTTTAGGTATGGATAGTTCATGGATGAATCGTAAAGGC
    GATGGCTATGAAGGATTTAAAAAAGAGTATGGTTTTGACTTTGGTACAGTGAGACCAATG
    CAAATAGGTCTAGTCTACGACGCATTAAACTCAGAGAAGTTAGACGTTGCATTAGGTTAT
    TCTACAGATGGTCGAATTGCGGCGTATGATTTGAAAGTACTTAAAGATGATAAACAATTT
    TTCCCACCTTATGCTGCGAGTGCTGTTGCAACAAATGAATTATTACGGCAACACCCAGAA
    CTTAAAACGACGATTAATAAGTTGACAGGAAAGATTTCGACTTCAGAGATGCAACGCTTG
    AATTATGAAGCGGATGGTAAAGGTAAAGAACCTGCTGTCGTCGCAGAAGAATTTTTGAAA
    AAACACCACTATTTTGATAAACAGAAAGGTGGTCATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    942

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2451 [new locus tag: SACOL_RS12865 ]
  • symbol: SACOL2451
  • description: amino acid ABC transporter amino acid-binding protein
  • length: 313
  • theoretical pI: 9.89386
  • theoretical MW: 34812.7
  • GRAVY: -0.4377

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsProtein interactionsPhr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 14.7)
  • TheSEED:  
    Stress ResponseOsmotic stressCholine and Betaine Uptake and Betaine Biosynthesis Osmotically activated L-carnitine/choline ABC transporter, substrate-binding protein OpuCC 
  • PFAM:
    PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 180)
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.9)
    no clan definedNrap_D2; Nrap protein PAP/OAS-like domain (PF17403; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: TVLSGCS
  • SignalP: Signal peptide length 31 aa
    • Ymax: 0.333
    • Ymax_pos: 31
    • Cmax: 0.174
    • Cmax_pos: 31
    • Smax: 0.871
    • Smax_pos: 14
    • Smean: 0.748
    • D: 0.495
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSESQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGALKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDLAKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVALGYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSEMQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]