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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02742
  • pan locus tag?: SAUPAN005998000
  • symbol: SAOUHSC_02742
  • pan gene symbol?: opuCC
  • synonym:
  • product: amino acid transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02742
  • symbol: SAOUHSC_02742
  • product: amino acid transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2521417..2522358
  • length: 942
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAGAAAATTAAATATATACTTGTCGTGTTTGTCTTATCGCTTACCGTATTATCTGGA
    TGTAGTTTGCCCGGACTAGGTAGTAAGAGCACGAAAAATGATGTCAAAATTACAGCATTA
    TCAACAAGCGAATCGCAAATTATTTCACATATGTTACGGTTGTTAATAGAGCATGATACA
    CACGGTAAGATAAAGCCAACATTAGTAAATAATTTAGGGTCAAGTACGATTCAACATAAT
    GCCTTAATTAATGGGGATGCTAATATATCAGGTGTTAGATATAATGGCACAGATTTAACG
    GGAGCTTTGAAGGAAGCACCAATTAAAAATCCTAAGAAAGCAATGATAGCAACACAACAA
    GGATTTAAAAAGAAATTTGATCAAACGTTTTTTGATTCGTATGGTTTTGCGAATACGTAT
    GCATTCATGGTAACGAAGGAAACCGCTAAAAAATATCATTTAGAGACAGTTTCAGATTTA
    GCAAAGCATAGTAAAGATTTACGTTTAGGTATGGATAGTTCATGGATGAATCGTAAAGGC
    GATGGCTATGAAGGATTTAAAAAAGAGTATGGTTTTGACTTTGGTACAGTGAGACCAATG
    CAAATAGGTCTAGTCTACGACGCATTAAACTCAGAGAAGTTAGACGTTGCATTAGGTTAT
    TCTACAGATGGTCGAATTGCGGCGTATGATTTGAAAGTACTTAAAGATGATAAACAATTT
    TTCCCACCTTATGCTGCGAGTGCTGTTGCAACAAATGAATTATTACGGCAACACCCAGAA
    CTTAAAACGACGATTAATAAGTTGACAGGAAAGATTTCGACTTCAGAGATGCAACGCTTG
    AATTATGAAGCGGATGGTAAAGGTAAAGAACCTGCTGTCGTCGCAGAAGAATTTTTGAAA
    AAACACCACTATTTTGATAAACAGAAAGGTGGTCATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    942

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02742
  • symbol: SAOUHSC_02742
  • description: amino acid transporter
  • length: 313
  • theoretical pI: 9.89386
  • theoretical MW: 34812.7
  • GRAVY: -0.4377

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsProtein interactionsPhr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 14.7)
  • TheSEED:  
    Stress ResponseOsmotic stressCholine and Betaine Uptake and Betaine Biosynthesis Osmotically activated L-carnitine/choline ABC transporter, substrate-binding protein OpuCC 
  • PFAM:
    PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 180)
    and 2 more
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.9)
    no clan definedNrap_D2; Nrap protein PAP/OAS-like domain (PF17403; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: TVLSGCS
  • SignalP: Signal peptide length 31 aa
    • Ymax: 0.333
    • Ymax_pos: 31
    • Cmax: 0.174
    • Cmax_pos: 31
    • Smax: 0.871
    • Smax_pos: 14
    • Smean: 0.748
    • D: 0.495
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSESQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGALKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDLAKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVALGYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSEMQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01228 regulon (CodY*)
    CodY*(TF)important in Amino acid metabolismRegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]