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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2591 [new locus tag: SACOL_RS13570 ]
  • pan locus tag?: SAUPAN006249000
  • symbol: SACOL2591
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2591 [new locus tag: SACOL_RS13570 ]
  • symbol: SACOL2591
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2653786..2654607
  • length: 822
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAGATATTTTAGTAATAGGCGCAACAGGGAAGCAAGGTAATGCAGTTGTAAAACAG
    TTGTTAGAGGATGGATGGTATGTAAGTGCATTAACTAGAAATAAGAATAATCGTAAACTT
    TCAGACATAGGACATCCGCATTTATCAATCGTTGAAGGTGATCTGAGTGATAATGTTAGT
    TTGCAATCAGCGATGAAAGGTAAGTATGGCTTATACAGTATTCAGCCAATCGTTAAAGAT
    GATGTTAGCGAAGAATTAAGACAAGGTATGAAGATAATTGAAATAGCTGAGCAAGAAAAT
    ATTCAACATATTGTGTATAGTACTGCGGGAGGGGTTAATCGAAATCGAACTGGCCCACAC
    TTTGAAGTACTAGCAAAAATAGAAAATAGATTAATGGAATCTAATATAAATGCAACAGTT
    ATCAAACCATCATTCTTTATGGATAATTTTTTACGCATTGCTAAAGTCGAAGATGAACGT
    ATTACATTACCAGAATTTATTAATCCGAATATAAAATTCACAATGATTTCTTCTATTGAT
    ATAGCTAAAATTGCATCGTATATTTTTGCACATCCACAAAGCTTTACGCATCAATCAATA
    GAGATTGGTTCCGATGAAGTTACATTAAGTGAAGCAGCAACAATTTTTAGTGAAGTGACA
    GGTAAATCTACTGTTATAGAAGGAGAATTTGTTAGTGGTGTTGCAGAAAAGCAATGGTTG
    GAAGAAAAAGGTTATGAAGTAGACTTTGAATTAATGGCTGAAATAAATCCAACAAGATTA
    TCATTAAGTGATTGGCTTAAAGTCCAAAACTATAATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    822

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2591 [new locus tag: SACOL_RS13570 ]
  • symbol: SACOL2591
  • description: hypothetical protein
  • length: 273
  • theoretical pI: 4.95399
  • theoretical MW: 30608.6
  • GRAVY: -0.237363

⊟Function[edit | edit source]

  • TIGRFAM:
    ergot alkaloid biosynthesis protein, AFUA_2G17970 family (TIGR03649; HMM-score: 42.7)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 41.9)
    and 4 more
    NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 16.4)
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 14.3)
    Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 13.7)
    Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 11.5)
  • TheSEED  :
    • NmrA-like protein
  • PFAM:
    NADP_Rossmann (CL0063) NmrA; NmrA-like family (PF05368; HMM-score: 135.7)
    and 9 more
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 76.5)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 30.5)
    3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 17.7)
    GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 17.3)
    adh_short; short chain dehydrogenase (PF00106; HMM-score: 16.2)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 13.5)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 12.9)
    DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 12.6)
    HTH (CL0123) HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007367
    • TAT(Tat/SPI): 0.000196
    • LIPO(Sec/SPII): 0.00085
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIGHPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQHIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDERITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSEVTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 38 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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