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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2612 [new locus tag: SACOL_RS13665 ]
  • pan locus tag?: SAUPAN006278000
  • symbol: SACOL2612
  • pan gene symbol?:
  • synonym:
  • product: CocE/NonD family hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2612 [new locus tag: SACOL_RS13665 ]
  • symbol: SACOL2612
  • product: CocE/NonD family hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 2668555..2670237
  • length: 1683
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAACCAACATTTACTAGGAAATCCAAAATTAACTGTAACTCATGTCAATGAAGTTAAA
    GCCGGTATTAACCACATCGTTGTCGACAGTGTTCAATATGGAAACCAAGAAATGATTATG
    GAAAAAGATGGCACTGTGGAAATGCGCGATGGCGAAAAATTATATATTAATATTTTCAGA
    CCAAATAAAGATGGCAAATTCCCTGTAGTTATGTCTGCAGATACTTACGGTAAAGATAAT
    AAGCCTAAAATCACAAATATGGGTGCCCTTTGGCCAACATTAGGTACCATTCCGACATCT
    AGTTTTACACCTGAAGAATCACCAGACCCAGGATTTTGGGTGCCAAATGATTATGTTGTA
    GTTAAAGTTGCATTACGCGGTAGTGACAAATCCAAAGGCGTCTTATCTCCATGGTCAAAA
    AGAGAAGCGGAAGATTATTACGAAGTGATTGAATGGGCAGCAAATCAGTCATGGAGTAAT
    GGAAATATCGGGACAAATGGTGTTTCTTATCTTGCGGTGACTCAATGGTGGGTCGCATCA
    TTAAATCCACCACATTTAAAAGCAATGATTCCTTGGGAAGGCTTAAATGATATGTATAGA
    GAAGTAGCCTTTCACGGAGGTATACCAGATACTGGCTTTTATCGTTTCTGGACTCAAGGT
    ATTTTTGCGAGATGGACAGATAATCCAAATATCGAAGATTTGATTCAAGCACAACAAGAA
    CATCCTCTGTTCGATGATTTTTGGAAACAGCGTCAAGTGCCATTATCACAAATTAAAACA
    CCTCTACTAACATGTGCTAGTTGGTCTACACAAGGTTTGCACAACCGTGGCTCTTTTGAA
    GGATTTAAACAAGCTGCATCTGAAGAAAAATGGCTATATGTGCATGGACGTAAAGAGTGG
    GAAAGTTACTACGCTAGAGAAAATCTCGAACGCCAAAAATCATTCTTTGATTTTTACCTT
    AAAGAAGAAAATAACGATTGGAAAGATACGCCTCATGTCATTTATGAAGTTAGAGATCAA
    TTTTATAAAGGCGAATTCAAATCAGCGTCAGCGTTCCCTTTACCTAACGCAGAATATACA
    CCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCGCGCAT
    GTCGCACAATATGACTCTGAAGATAAACAACAAGATGTAAGTTTTAAATATACGTTTGAC
    AAAGATACTGAGTTAGTTGGAAACATGAACTTAAAACTATGGGTAAGCACTAAAGACTCA
    GATGATATGGATTTATTTGCAGGTATTAAAAAGTTAGATCGTCGTGGTAATGAAGTTAAC
    TTCCCTGATTTTAATCATATTGAAAATGGTCAAGTAGCTACTGGTTGGTTACGCGTATCA
    CATCGTGAATTAGATCAAGAAAAATCCTCAATCGCGCAACCTTGGCATAAACATGAAACA
    GAATTAAAGTTGTCACAAGATGAGATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGC
    ACGCTATTTAAACAAGGCGAAACATTGGAAGTTGTTGTAAAGGGTAGTGAAATTGTAATT
    GGTAATAGTACTCCTGGCATGAAAACACGTTATGAACATGAAGAAACCGTAAATAAAGGC
    ATGCACATGATTTATACTGGTGGTAAATATGATTCACAATTAATCATTCCTATCGTTAAT
    TGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1683

Protein[edit source | edit]

Protein Data Bank: 3IB3
Protein Data Bank: 3III

General[edit source | edit]

  • locus tag: SACOL2612 [new locus tag: SACOL_RS13665 ]
  • symbol: SACOL2612
  • description: CocE/NonD family hydrolase
  • length: 560
  • theoretical pI: 5.29295
  • theoretical MW: 64247.5
  • GRAVY: -0.6375

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityhydrolase CocE/NonD family protein (TIGR00976; HMM-score: 255.6)
  • TheSEED:  
    Predicted acyl esterase/dipeptidyl-peptidase 
  • PFAM:
    GBD (CL0202) PepX_C; X-Pro dipeptidyl-peptidase C-terminal non-catalytic domain (PF08530; HMM-score: 162)
    AB_hydrolase (CL0028) Peptidase_S15; X-Pro dipeptidyl-peptidase (S15 family) (PF02129; HMM-score: 142.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.185
    • Ymax_pos: 22
    • Cmax: 0.162
    • Cmax_pos: 22
    • Smax: 0.269
    • Smax_pos: 20
    • Smean: 0.217
    • D: 0.198
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDGTVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWTQGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQDVSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL2612 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]