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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS00345 [old locus tag: SACOL0067 ]
  • pan locus tag?: SAUPAN000382000
  • symbol: SACOL_RS00345
  • pan gene symbol?:
  • synonym:
  • product: tRNA-dihydrouridine synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS00345 [old locus tag: SACOL0067 ]
  • symbol: SACOL_RS00345
  • product: tRNA-dihydrouridine synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 76615..77547
  • length: 933
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (76615..77547) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAAGACGTTACAGATATCGTCTTTCGACACGTTGTAAGTGAAGCAGCTAGACCGGAT
    GTGTTTTTCACTGAATTTACAAATACTGAAAGCTTTTGCCACCCTGAAGGCATACATAGT
    GTGCGCGGACGCTTAACTTTTAGTGAAGATGAACAGCCGATGGTCGCTCATATATGGGGA
    GATAAGCCAGAACAGTTCCGTGAAACGAGTATTCAATTAGCTAAAATGGGCTTTAAAGGC
    ATAGACTTAAATATGGGATGTCCTGTAGCAAATGTTGCTAAAAAGGGTAAGGGTTCCGGC
    TTAATCTTAAGACCTGACGTTGCTGCCGAAATTATTCAAGCGACTAAAGCAGGTGGGCTT
    CCGGTAAGTGTTAAAACACGCCTTGGCTACTATGAAATCGATGAATGGAAAGATTGGTTG
    AAGCACGTCTTCGAACAAGACATTGCCAATTTATCTATTCATCTTCGTACACGTAAAGAA
    ATGAGTAAAGTAGATGCACATTGGGAATTAATCGAAGCTATTAAAAATTTACGTGACGAA
    ATTGCACCAAATACATTGTTAACAATTAACGGTGATATTCCCGATAGAAAAACAGGACTT
    GAACTGGCAGAAAAATATGGCATTGATGGCGTCATGATTGGTAGAGGCATTTTCCACAAT
    CCATTCGCTTTTGAAAAAGAACCACGCGAACACACAAGCAAGGAACTATTAGATCTATTG
    AGATTGCATTTATCATTGTTTAACAAATATGAAAAAGATGAAATACGACAATTCAAGAGC
    TTGCGTAGATTCTTTAAAATCTATGTGCGTGGCATAAGAGGCGCTAGCGAACTTCGACAT
    CAATTGATGAACACACAATCAATTGCAGAAGCACGAGCACTACTCGATGAATTTGAAGCC
    CAAATGGACGAAGACGTTAAAATTGAATTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS00345 [old locus tag: SACOL0067 ]
  • symbol: SACOL_RS00345
  • description: tRNA-dihydrouridine synthase
  • length: 310
  • theoretical pI: 6.14661
  • theoretical MW: 35562.5
  • GRAVY: -0.393548

Function[edit source | edit]

  • reaction:
    EC 1.3.1.-?  ExPASy
    5,6-dihydrouracil in tRNA + NAD(P)+ = uracil in tRNA + NAD(P)H?
  • TIGRFAM:
    Unknown functionGeneralputative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 152.5)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA dihydrouridine synthase A (TIGR00742; EC 1.-.-.-; HMM-score: 83.7)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 19.3)
    Unknown functionGeneralputative TIM-barrel protein (TIGR00736; HMM-score: 15.6)
  • TheSEED:
  • PFAM:
    TIM_barrel (CL0036) Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 160.8)
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 16.1)
    TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 13.8)
    no clan definedMPS2; Monopolar spindle protein 2 (PF17060; HMM-score: 12.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FMN
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 35
    • Cmax: 0.125
    • Cmax_pos: 59
    • Smax: 0.133
    • Smax_pos: 16
    • Smean: 0.083
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_001827266 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS00345 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]