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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS01375 [old locus tag: SACOL0271 ]
  • pan locus tag?: SAUPAN001175000
  • symbol: SACOL_RS01375
  • pan gene symbol?: esxA
  • synonym:
  • product: virulence factor EsxA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS01375 [old locus tag: SACOL0271 ]
  • symbol: SACOL_RS01375
  • product: virulence factor EsxA
  • replicon: chromosome
  • strand: +
  • coordinates: 309844..310137
  • length: 294
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (309844..310137) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    ATGGCAATGATTAAGATGAGTCCAGAGGAAATCAGAGCAAAATCGCAATCTTACGGGCAA
    GGTTCAGACCAAATCCGTCAAATTTTATCTGATTTAACACGTGCACAAGGTGAAATTGCA
    GCGAACTGGGAAGGTCAAGCTTTCAGCCGTTTCGAAGAGCAATTCCAACAACTTAGTCCT
    AAAGTAGAAAAATTTGCACAATTATTAGAAGAAATTAAACAACAATTGAATAGCACTGCT
    GATGCCGTTCAAGAACAAGACCAACAACTTTCTAATAATTTCGGTTTGCAATAA
    60
    120
    180
    240
    294

Protein[edit source | edit]

Protein Data Bank: 2VRZ
Protein Data Bank: 2VS0

General[edit source | edit]

  • locus tag: SACOL_RS01375 [old locus tag: SACOL0271 ]
  • symbol: SACOL_RS01375
  • description: virulence factor EsxA
  • length: 97
  • theoretical pI: 4.32285
  • theoretical MW: 11036.2
  • GRAVY: -0.765979

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    WXG100 family type VII secretion target (TIGR03930; HMM-score: 80.2)
    type VII secretion effector, TIGR04197 family (TIGR04197; HMM-score: 20.8)
    two transmembrane protein (TIGR04527; HMM-score: 18.8)
    putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 13.3)
    Genetic information processingProtein fateProtein folding and stabilizationprefoldin, alpha subunit (TIGR00293; HMM-score: 10.3)
  • TheSEED:
  • PFAM:
    EsxAB (CL0352) WXG100; Proteins of 100 residues with WXG (PF06013; HMM-score: 78.1)
    T7SS_ESX_EspC; Excreted virulence factor EspC, type VII ESX diderm (PF10824; HMM-score: 19.3)
    no clan definedApolipoprotein; Apolipoprotein A1/A4/E domain (PF01442; HMM-score: 18.7)
    EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 18.3)
    KxDL; Uncharacterized conserved protein (PF10241; HMM-score: 18.3)
    DUF2130; Uncharacterized protein conserved in bacteria (DUF2130) (PF09903; HMM-score: 17.4)
    IFT57; Intra-flagellar transport protein 57 (PF10498; HMM-score: 16.8)
    Med2; Mediator complex subunit 2 (PF11214; HMM-score: 16.6)
    TSNAXIP1_N; Translin-associated factor X-interacting N-terminus (PF15739; HMM-score: 16.6)
    Filament; Intermediate filament protein (PF00038; HMM-score: 15.9)
    Tup_N; Tup N-terminal (PF08581; HMM-score: 15.8)
    Cast; RIM-binding protein of the cytomatrix active zone (PF10174; HMM-score: 15.7)
    ING; Inhibitor of growth proteins N-terminal histone-binding (PF12998; HMM-score: 15.1)
    Phage_30_3; Bacteriophage protein GP30.3 (PF08010; HMM-score: 15)
    DNA_repr_REX1B; DNA repair REX1-B (PF14966; HMM-score: 15)
    LXG; LXG domain of WXG superfamily (PF04740; HMM-score: 14.8)
    Mobilization_B; Mobilization protein B (PF17511; HMM-score: 14.8)
    PDDEXK (CL0236) RmuC; RmuC family (PF02646; HMM-score: 14.7)
    no clan definedCK2S; Casein Kinase 2 substrate (PF15011; HMM-score: 14.6)
    TRPM_tetra; Tetramerisation domain of TRPM (PF16519; HMM-score: 14.6)
    DUF5344; Family of unknown function (DUF5344) (PF17279; HMM-score: 14.6)
    Skp1; Skp1 family, dimerisation domain (PF01466; HMM-score: 13.6)
    Cytochrom_B562; Cytochrome b562 (PF07361; HMM-score: 12.6)
    MscS_porin; Mechanosensitive ion channel porin domain (PF12795; HMM-score: 11.9)
    OML_zippers (CL0590) LPP; Lipoprotein leucine-zipper (PF04728; HMM-score: 11.7)
    no clan definedUPF0449; Uncharacterised protein family UPF0449 (PF15136; HMM-score: 11.7)
    FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 10.8)
    TMPIT; TMPIT-like protein (PF07851; HMM-score: 10.5)
    KNOX2; KNOX2 domain (PF03791; HMM-score: 10.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 14
    • Cmax: 0.128
    • Cmax_pos: 41
    • Smax: 0.176
    • Smax_pos: 3
    • Smean: 0.116
    • D: 0.116
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447163570 NCBI
  • RefSeq: WP_001240826 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWEGQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS01375 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]