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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03100 [old locus tag: SACOL0598 ]
  • pan locus tag?: SAUPAN002324000
  • symbol: SACOL_RS03100
  • pan gene symbol?: araB
  • synonym:
  • product: ribulokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS03100 [old locus tag: SACOL0598 ]
  • symbol: SACOL_RS03100
  • product: ribulokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 623461..625098
  • length: 1638
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (623461..625098) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
    421
    481
    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTTATAGCATTGGAATTGATTATGGAACTGCTTCAGGCCGTGTGTTTTTAATTAAT
    ACAACTAACGGTCAAGTAGTATCAAAATTTGTGAAGCCATATACACATGGTGTCATTGAG
    AGTGAATTAAATGGTTTGAAAATACCACATACATATGCACTTCAAAATAGTAATGATTAT
    TTAGAAATTATGGAAGAAGGAATATCATATATAGTACGTGAATCAAAAATAGATCCAGAC
    AATATAGTAGGTATTGGTATAGACTTTACTTCATCTACTATTATTTTTACTGACGAAAAC
    CTTAACCCGGTACATAACTTAAAACAATTTAAAAACAATCCACATGCGTATGTGAAACTT
    TGGAAACATCATGGTGCATATAAAGAGGCAGAGAAATTATATCAAACTGCTATTGAAAAT
    AATAATAAGTGGTTAGGCCATTATGGATATAATGTTAGTAGTGAATGGATGATTCCCAAA
    ATAATGGAGGTCATGAATCGAGCACCAGAAATTATGGAAAAAACGGCTTATATTATGGAA
    GCGGGCGATTGGATTGTAAATAAATTAACTAATAAAAATGTACGCTCGAATTGTGGATTA
    GGTTTCAAAGCATTTTGGGAAGAAGAAACAGGGTTTCATTATGATTTATTTGATAAAATA
    GACCCCAAATTATCAAAAGTAATTCAAGATAAAGTATCTGCACCGGTTGTTAATATTGGT
    GAAGCAGTAGGGAAACTGGATGATAAAATGGCACAGAAATTAGGATTATCAAAAGAAACT
    ATGGTAAGTCCTTTTATTATTGATGCCCATGCTAGTTTATTAGGTATTGGGTCTGAAAAA
    GATAAAGAAATGACTATGGTGATGGGAACAAGCACATGCCATCTTATGTTAAATGAAAAG
    CAACATCAAGTGCCAGGTATATCAGGTTCTGTAAAAGGAGCAATTATTCCAGAATTATTT
    GCTTATGAAGCGGGGCAATCAGCAGTAGGTGATTTGTTTGAGTATGTCGCTAAGCAAGCA
    CCAAAGTCATATGTAGATGAAGCAGAAAATAGAAATATGACTGTATTTGAATTAATGAAT
    GAAAAGATAAAACATCAAATGCCAGGTGAAAGTGGGCTCATTGCTCTTGATTGGCATAAT
    GGAAATCGAAGTGTATTAAGTGATAGCAATTTAACAGGTTGTATCTTTGGATTAACTTTA
    CAAACTAAGCATGAGGATATTTATAGAGCATATTTAGAAGCTACAGCATTTGGTACTAAG
    ATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGC
    GGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAA
    CTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTC
    AGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTA
    TACGAAATTAATCCAGAAGCGGAAAAAGTACAAAGGTATGAAACATTATTTAAAGCTTAT
    AAGGCTTTACATGATATCCATGGTTATAAAAAAGCTAATATAATGAAAGATATCCAGAGT
    TTAAGAGTTGAGGGATAA
    60
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    1260
    1320
    1380
    1440
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    1560
    1620
    1638

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS03100 [old locus tag: SACOL0598 ]
  • symbol: SACOL_RS03100
  • description: ribulokinase
  • length: 545
  • theoretical pI: 6.04212
  • theoretical MW: 61010.4
  • GRAVY: -0.278165

Function[edit source | edit]

  • reaction:
    EC 2.7.1.16?  ExPASy
    RibulokinaseATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismSugarsribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 557)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 209.7)
    MetabolismEnergy metabolismSugarsxylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 154.3)
    MetabolismEnergy metabolismSugarsgluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 86.9)
    MetabolismEnergy metabolismOtherglycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 68.4)
    MetabolismEnergy metabolismSugarsL-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 58.6)
  • TheSEED:
  • PFAM:
    Actin_ATPase (CL0108) FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 126)
    FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 63.3)
    no clan definedDUF4846; Domain of unknown function (4846) (PF16138; HMM-score: 17.5)
    Actin_ATPase (CL0108) BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 13.3)
    Concanavalin (CL0004) SPRY; SPRY domain (PF00622; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.64
    • Cytoplasmic Membrane Score: 0.3
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.189
    • Ymax_pos: 30
    • Cmax: 0.17
    • Cmax_pos: 30
    • Smax: 0.367
    • Smax_pos: 28
    • Smean: 0.148
    • D: 0.173
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446044485 NCBI
  • RefSeq: WP_000122340 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELNGLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLNPVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPKIMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFDKIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGIGSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFEYVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLTGCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMMDIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAEKVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS03100 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]