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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03235 [old locus tag: SACOL0626 ]
  • pan locus tag?: SAUPAN002357000
  • symbol: SACOL_RS03235
  • pan gene symbol?: pdxK
  • synonym:
  • product: hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS03235 [old locus tag: SACOL0626 ]
  • symbol: SACOL_RS03235
  • product: hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 658750..659580
  • length: 831
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (658750..659580) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTTTAAAGAAAGTTTTAACAATTGCCGGTTCTGACACAAGTGCTGGCGCAGGTATG
    CAAGCAGATTTGAAAACGTTCCAAGAATTAGATACGTATGGCATGGTCGCTTTAACTGCC
    ATCGTTACTATGGATAAAGATACATGGTCACACGATGTTACACCATTACCAATGGATGTA
    TTTGAAAAACAACTTGAAACGGCTTTATCAATCGGACCTGATGCTATTAAAACAGGTATG
    TTAGGTACTGAGGAGATAATCAAACGTGCTGGAGAAGTATATGAGGCATCTAATGCACAA
    TATTTTGTAGTAGATCCTGTCATGGTTTGTAAAGGCGAAGATGAAGTGCTTAATCCTGGA
    AATACTGAGGCGATGATTAAATATTTACTACCTAAAGCAACGGTAGTGACACCAAACTTA
    TTTGAAGCGGGTCAATTATCAGGATTAGGTAAATTAAATTCAATTGAAGATATGAAAAAG
    GCTGCAACAATTATCTTTGATAAAGGCGCACAGCATGTCATCATTAAAGGTGGCAAAGCT
    TTAGATCAAGATAAATCGTATGACTTATACTATGATGGTCAAACATTTTATCAACTAACA
    ACAGACATGTTCCAACAAAGTTATAACCATGGTGCTGGTTGTACATTTGCTGCGGCAACA
    ACTGCATATTTAGCTAACGGTAAGTCACCGAAAGAAGCTGTGATTAGCGCGAAAGCTTTC
    GTCGCTTCTGCTATTAAAAACGGTTGGAAAATGAATGATTTTGTTGGTCCTGTGGATCAC
    GGTGCATACAACCGTATTGAACATATCGATGTTGAAGTAACAGAGGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    831

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS03235 [old locus tag: SACOL0626 ]
  • symbol: SACOL_RS03235
  • description: hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
  • length: 276
  • theoretical pI: 4.61494
  • theoretical MW: 29856.8
  • GRAVY: -0.105435

Function[edit source | edit]

  • reaction:
    EC 2.7.4.7?  ExPASy
    Phosphomethylpyrimidine kinaseATP + 4-amino-2-methyl-5-(phosphomethyl)pyrimidine = ADP + 4-amino-2-methyl-5-(diphosphomethyl)pyrimidine
    EC 2.7.1.35?  ExPASy
    Pyridoxal kinaseATP + pyridoxal = ADP + pyridoxal 5'-phosphate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminehydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 235.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxinepyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 54.9)
    MetabolismEnergy metabolismSugarsribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 38.4)
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 27.2)
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 24.1)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesbifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 20.5)
    MetabolismEnergy metabolismSugars5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 17.6)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidestagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 13.4)
  • TheSEED:
  • PFAM:
    Ribokinase (CL0118) Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 260.5)
    PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 39.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.21
    • Ymax_pos: 23
    • Cmax: 0.152
    • Cmax_pos: 23
    • Smax: 0.525
    • Smax_pos: 16
    • Smean: 0.321
    • D: 0.253
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447108206 NCBI
  • RefSeq: WP_001185462 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS03235 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]