From AureoWiki
Jump to: navigation, search
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04400 [old locus tag: SACOL0857 ]
  • pan locus tag?: SAUPAN002791000
  • symbol: SACOL_RS04400
  • pan gene symbol?: vwb
  • synonym:
  • product: coagulase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS04400 [old locus tag: SACOL0857 ]
  • symbol: SACOL_RS04400
  • product: coagulase
  • replicon: chromosome
  • strand: +
  • coordinates: 883665..885191
  • length: 1527
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (883665..885191) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    TTGAAAAATAAATTGCTAGTTTTATCATTGGGAGCATTATGTGTATCACAAATTTGGGAA
    AGTAATCGTGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCTGAGTCGTTG
    AAACTGACTAATAATAAAAATAAATCTAGAACAGTAGAAGAGTATAAGAAAAGCTTGGAT
    GATTTAATATGGTCCTTTCCAAACTTAGATAATGAAAGATTTGATAATCCTGAATATAAA
    GAAGCTATGAAAAAATATCAACAGAGATTTATGGCTGAAGATGAGGCTTTGAAGAAATTT
    TTTAGTGAAGAGAAAAAAATAAAAAATGGAAATACTGATAATTTAGATTATCTAGGATTA
    TCTCATGAAAGATATGAAAGTGTATTTAATACTTTGAAAAAACAAAGTGAGGAGTTCTTA
    AAAGAAATTGAAGATATAAAAAAAGATAACCCTGAATTGAAAGACTTTAATGAAGAGGAG
    CAATTAAAGTGCGACTTAGAATTAAACAAATTAGAAAATCAGATATTAATGTTAGGTAAA
    ACATTTTATCAAAACTATAGAGATGATGTTGAAAGTTTATATAGTAAGTTAGATTTAATT
    ATGGGATATAAAGATGAAGAAAGAGCAAATAAAAAAGCAGTTAACAAAAGGATGTTAGAA
    AATAAAAAAGAAGACTTAGAAACCATAATTGATGAATTTTTTAGTGATATAGATAAAACA
    AGACCTAATAATATTCCTGTTTTAGAAGATGAAAAACAAGAAGAGAAAAATCATAAAAAT
    ATGGCTCAATTAAAATCTGACACTGAAGCAGCAAAAAGTGATGAATCAAAAAGAAGCAAG
    AGAAGTAAAAGAAGTTTAAATACTCAAAATCACAAACCTGCATCTCAAGAAGTTTCTGAA
    CAACAAAAAGCTGAATATGATAAAAGAGCAGAAGAAAGAAAAGCGAGATTTTTGGATAAT
    CAAAAAATTAAGAAAACACCTGTAGTGTCATTAGAATATGATTTTGAGCATAAACAACGT
    ATTGACAACGAAAACGACAAGAAACTTGTGGTTTCTGCACCAACAAAGAAACCAACATCA
    CCGACTACATATACTGAAACAACGACACAGGTACCAATGCCTACAGTTGAGCGTCAAACT
    CAGCAACAAATTATTTATAATGCACCAAAACAATTGGCTGGATTAAATGGTGAAAGTCAT
    GATTTCACAACAACGCATCAATCACCAACAACTTCAAATCACACGCATAATAATGTTGTT
    GAATTTGAAGAAACGTCTGCTTTACCTGGTAGAAAATCAGGATCACTGGTTGGTATAAGT
    CAAATTGATTCTTCTCATCTAACTGAACGTGAGAAGCGTGTAATTAAGCGTGAACACGTT
    AGAGAAGCTCAAAAGTTAGTTGATAATTATAAAGATACACATAGTTATAAAGACCGAATA
    AATGCACAACAAAAAGTAAATACTTTAAGTGAAGGTCATCAAAAACGTTTTAATAAACAA
    ATCAATAAAGTATATAATGGCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1527

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS04400 [old locus tag: SACOL0857 ]
  • symbol: SACOL_RS04400
  • description: coagulase
  • length: 508
  • theoretical pI: 8.16958
  • theoretical MW: 59277.8
  • GRAVY: -1.18642

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:
  • PFAM:
    no clan definedCoagulase; Staphylococcus aureus coagulase (PF08764; HMM-score: 68.9)
    efb-c; Extracellular fibrinogen binding protein C terminal (PF12199; HMM-score: 7.8)
    EF-G-binding_N; Elongation factor G-binding protein, N-terminal (PF07299; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 24 aa
    • Ymax: 0.466
    • Ymax_pos: 24
    • Cmax: 0.284
    • Cmax_pos: 24
    • Smax: 0.875
    • Smax_pos: 18
    • Smean: 0.717
    • D: 0.564
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446714398 NCBI
  • RefSeq: WP_000791729 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTNNKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFSEEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEEQLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRMLENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDESKRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYDFEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQLAGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS04400 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]