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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04845 [old locus tag: SACOL0945 ]
  • pan locus tag?: SAUPAN003041000
  • symbol: SACOL_RS04845
  • pan gene symbol?: pepZ
  • synonym:
  • product: cytosol aminopeptidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS04845 [old locus tag: SACOL0945 ]
  • symbol: SACOL_RS04845
  • product: cytosol aminopeptidase
  • replicon: chromosome
  • strand: +
  • coordinates: 946750..948225
  • length: 1476
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (946750..948225) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    1021
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    1441
    ATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATA
    CCAGAACATTTAAATCAGTTAGAGCGCATTAGTTTTAATCATATCGATATTACAGAATCA
    CTTGAAAGACTAAAACATCAACATATTATTGGTAGTAAAGTTGGGAAGATTTATACAACT
    GCATTTGATGTACAAGATCAAACATATCGTTTAATTACAGTTGGTTTAGGAAACTTAAAG
    ACACGTAGTTATCAAGATATGTTGAAAATATGGGGACATCTTTTCCAATACATAAAGTCA
    GAACACATTGAAGATACGTATTTACTTATGGATTCATTTATTTCAAAATATGATCAGTTA
    TCAGATGTATTAATGGCATGCGGTATTCAAAGTGAGCGTGCAACATATGAATTCGATCAT
    TATAAATCAAGTAAGAAGGCACCGTTTAAGACGAATTTAAACCTTATTAGTGAATCATTA
    ATTGAATTAGATTTTATTCATGAGGGTATCAGTATTGGCCAATCCATTAATTTGGCAAGA
    GACTTTAGTAATATGCCACCGAATGTATTAACACCACAAACATTTGCAGAAGATATTGTT
    AATCATTTTAAAAATACAAAGGTCAAAGTAGATGTTAAAGATTATGACACTTTAGTTTCT
    GAAGGATTCGGACTTTTACAAGCAGTAGGTAAAGGTAGTAAGCATAAACCGAGATTAGTA
    ACCATCACATATAATGGCAAAGACAAAGATGAAGCACCAATTGCCTTAGTTGGTAAAGGT
    ATAACGTATGATTCTGGTGGTTATAGTATTAAAACGAAGAATGGCATGGCTACAATGAAG
    TTTGACATGTGTGGCGCTGCGAATGTCGTTGGTATCATTGAAGCGGCTAGTCGTTTACAA
    CTGCCTGTAAATATTGTCGGAGTGCTTGCGTGTGCTGAAAATATGATAAATGAAGCATCA
    ATGAAGCCAGATGATGTATTTACAGCATTAAGTGGTGAAACTGTAGAAGTAATGAATACA
    GACGCTGAAGGTAGATTAGTCCTTGCAGATGCTGTGTTTTATGCAAATCAATATCAGCCT
    AGTGTGATTATGGACTTTGCTACATTAACGGGTGCAGCAATTGTTGCACTAGGCGATGAT
    AAAGCTGCTGCATTTGAATCGAATAGTAAAGTGATATTAAACGATATATTACAAATAAGT
    TCTGAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATT
    AAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTT
    GCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCA
    GGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATG
    ATTCCGACGATAGTACAATGGTTAAAACAACAATAA
    60
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    1476

Protein[edit source | edit]

Protein Data Bank: 3KZW

General[edit source | edit]

  • locus tag: SACOL_RS04845 [old locus tag: SACOL0945 ]
  • symbol: SACOL_RS04845
  • description: cytosol aminopeptidase
  • length: 491
  • theoretical pI: 5.71046
  • theoretical MW: 54128.3
  • GRAVY: -0.116904

Function[edit source | edit]

  • reaction:
    EC 3.4.11.1?  ExPASy
    Leucyl aminopeptidaseRelease of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low
  • TIGRFAM:
  • TheSEED:
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M17; Cytosol aminopeptidase family, catalytic domain (PF00883; HMM-score: 358.8)
    MACRO (CL0223) Peptidase_M17_N; Cytosol aminopeptidase family, N-terminal domain (PF02789; HMM-score: 61.5)
    no clan definedTerD; TerD domain (PF02342; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.135
    • Ymax_pos: 12
    • Cmax: 0.11
    • Cmax_pos: 30
    • Smax: 0.197
    • Smax_pos: 8
    • Smean: 0.178
    • D: 0.152
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446932441 NCBI
  • RefSeq: WP_001009697 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERLKHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEHIEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESLIELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTLVSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGMATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGETVEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVILNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSGQTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS04845 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]