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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS06940 [old locus tag: SACOL1364 ]
  • pan locus tag?: SAUPAN003702000
  • symbol: SACOL_RS06940
  • pan gene symbol?: thrB
  • synonym:
  • product: homoserine kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS06940 [old locus tag: SACOL1364 ]
  • symbol: SACOL_RS06940
  • product: homoserine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1369542..1370456
  • length: 915
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1369542..1370456) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCGAATGTTTTGGAGTTAACAATTCCTGCATCAACAGCCAACCTTGGAGTTGGCTTT
    GATTCTATAGGTATGGCTTTAGATAAATTTTTGCATCTGTCTGTAAAGGAAACATCAGGG
    ACAAAATGGGAATATATTTTCCATGATGATGCATCTAAGCAATTGCCTACTGACGAAACA
    AACTTTATTTATCATGTAGCACAACAAGTTGCTTCTAAATATAGTGTTGACTTGCCTAAT
    TTATGTATCGAAATGAGAAGTGATATTCCATTGGCAAGAGGGTTAGGTTCGTCAGCTTCT
    GCTTTAGTAGGAGCTATATATATCGCAAATTATTTTGGTGATATCCAACTGTCTAAACAT
    GAGGTATTACAATTAGCGACTGAAATCGAAGGACATCCTGATAATGTTGCGCCGACCATT
    TATGGTGGTTTAATCGCTGGATATTATAATGATGTCTCGAAAGAAACGTCAGTTGCACAT
    ATCGACATACCAGACGTGGATGTGATTGTAACGATACCAACTTATGAACTAAAAACAGAA
    GCATCAAGACGTGCTTTACCACAAAAATTAACACATAGTGAAGCGGTTAAAAGTAGTGCA
    ATTAGTAATACAATGATTTGTGCATTAGCACAGCACAATTATGAATTAGCAGGTAAACTC
    ATGCAACAAGATGGCTTTCATGAACCGTATCGTCAGCATTTAATTGCTGAATTTGATGAA
    GTGAAAACAATTGCTAGTCAACATAATGCCTATGCAACTGTAATTAGTGGTGCTGGACCA
    ACTATTTTAATATTTAGTCGTAAAGAAAATAGTGGGGAATTGGTTCGCTCTTTAAATAGT
    CAGGTAGTATCATGCCATTCTGAATTGGTCGATATTAATATCAGTGGTGTTAAAGAACGA
    ATTGTATACCAATAG
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS06940 [old locus tag: SACOL1364 ]
  • symbol: SACOL_RS06940
  • description: homoserine kinase
  • length: 304
  • theoretical pI: 5.15013
  • theoretical MW: 33266.4
  • GRAVY: -0.0641447

Function[edit source | edit]

  • reaction:
    EC 2.7.1.39?  ExPASy
    Homoserine kinaseATP + L-homoserine = ADP + O-phospho-L-homoserine
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAspartate familyhomoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 260.2)
    MetabolismCentral intermediary metabolismOthermevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 47)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 29.9)
    MetabolismAmino acid biosynthesisAromatic amino acid familyshikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 28.8)
    MetabolismCentral intermediary metabolismOtherphosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 22.9)
    MetabolismCentral intermediary metabolismOtherdiphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 16.9)
    MetabolismEnergy metabolismSugarsgalactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 12.5)
  • TheSEED:
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 52.9)
    no clan definedGHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 34.9)
    DUF3963; Protein of unknown function (DUF3963) (PF13124; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.151
    • Ymax_pos: 27
    • Cmax: 0.153
    • Cmax_pos: 27
    • Smax: 0.293
    • Smax_pos: 1
    • Smean: 0.164
    • D: 0.156
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445995329 NCBI
  • RefSeq: WP_000073184 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWEYIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASALVGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAHIDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAGKLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVRSLNSQVVSCHSELVDINISGVKERIVYQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS06940 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]