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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07010 [old locus tag: SACOL1377 ]
  • pan locus tag?: SAUPAN003717000
  • symbol: SACOL_RS07010
  • pan gene symbol?: tkt
  • synonym:
  • product: transketolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07010 [old locus tag: SACOL1377 ]
  • symbol: SACOL_RS07010
  • product: transketolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1380327..1382315
  • length: 1989
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1380327..1382315) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
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    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTTAATGAAAAAGATCAATTAGCTGTTGATACGCTACGTGCACTAAGTATCGACACA
    ATCGAAAAAGCGAATTCTGGTCATCCAGGATTACCTATGGGAGCTGCCCCAATGGCTTAC
    ACTTTGTGGACACGTCATCTGAATTTTAATCCACAATCTAAAGATTACTTCAATAGAGAC
    CGTTTCGTATTATCTGCAGGGCATGGTTCAGCATTATTGTATAGCTTGTTACATGTTTCT
    GGTAGTTTAGAATTAGAAGAATTAAAGCAATTTAGACAATGGGGTTCTAAAACACCAGGT
    CATCCTGAATACAGACATACAGATGGTGTAGAAGTTACTACCGGACCACTTGGACAAGGT
    TTTGCTATGTCAGTAGGATTAGCTTTAGCAGAAGATCACCTAGCAGGGAAATTTAATAAA
    GAAGGATATAATGTTGTAGATCATTACACATATGTATTAGCTTCTGACGGTGATTTAATG
    GAAGGTATATCGCATGAAGCAGCTTCATTTGCTGGACATAATAAATTAAGTAAATTAGTT
    GTTTTATACGATTCAAATGATATTTCATTAGATGGCGAATTAAACAAAGCTTTTTCTGAA
    AACACAAAAGCTCGTTTTGAAGCATATGGTTGGAATTACTTACTAGTTAAAGATGGTAAT
    GATTTAGAAGAAATTGATAAAGCGATTACTACAGCTAAATCTCAAGAAGGACCAACGATT
    ATTGAAGTTAAAACAACAATCGGATTTGGTTCACCGAATAAAGCAGGAACTAATGGTGTT
    CATGGGGCACCTTTAGGTGAAGTTGAAAGAAAATTAACATTCGAAAATTACGGTTTAGAT
    CCTGAAAAACGTTTTAATGTTTCAGAAGAGGTATACGAAATTTTCCAAAATACTATGTTA
    AAACGTGCTAATGAAGATGAATCTCAATGGAATTCATTATTAGAAAAATATGCAGAAACA
    TATCCTGAATTAGCAGAAGAATTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTAT
    AAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGCTGATTCTGGT
    ACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTT
    GCTGGTTCAAACAAATCCAATGTAAATGATGCAACTGATTATAGTTCTGAAACACCTGAA
    GGTAAAAATGTGTGGTTTGGTGTACGTGAATTTGCTATGGGTGCTGCTGTAAATGGTATG
    GCTGCACATGGAGGTTTACATCCATATGGTGCAACATTCTTCGTATTTAGTGATTATTTA
    AAACCAGCGTTACGTTTATCATCAATTATGGGATTAAATGCAACGTTCATCTTCACACAT
    GATTCAATTGCAGTAGGTGAAGATGGTCCTACTCATGAACCAATTGAGCAATTAGCTGGA
    TTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTA
    GCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAA
    AACTTACCGGTATTAGATGTACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCC
    TATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAA
    GTTAGTCTTGCAGTTGAAGCTGCTAAAGATCTTGAAAAACAAGGTAAATCAGTACGTGTT
    GTTTCAATGCCTAACTGGAATGCATTTGAACAACAATCTGAAGAATATAAAGAATCAGTT
    ATTCCATCAAGCGTAACAAAACGTGTTGCGATTGAAATGGCTTCACCGCTTGGATGGCAT
    AAATATGTAGGTACTGCAGGTAAAGTTATTGCTATTGACGGCTTTGGCGCAAGTGCACCT
    GGCGATTTAGTAGTTGAAAAATATGGATTTACAAAAGAAAATATCTTAAACCAAGTTATG
    AGCTTATAA
    60
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    1560
    1620
    1680
    1740
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    1860
    1920
    1980
    1989

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07010 [old locus tag: SACOL1377 ]
  • symbol: SACOL_RS07010
  • description: transketolase
  • length: 662
  • theoretical pI: 4.70733
  • theoretical MW: 72250.3
  • GRAVY: -0.332326

Function[edit source | edit]

  • reaction:
    EC 2.2.1.1?  ExPASy
    TransketolaseSedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismPentose phosphate pathwaytransketolase (TIGR00232; EC 2.2.1.1; HMM-score: 915.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74.6)
    pyruvate dehydrogenase (acetyl-transferring), homodimeric type (TIGR00759; EC 1.2.4.1; HMM-score: 34.3)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 20.8)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 17.6)
    alpha-ketoglutarate dehydrogenase (TIGR03186; HMM-score: 15.6)
  • TheSEED:
  • PFAM:
    THDP-binding (CL0254) Transketolase_N; Transketolase, thiamine diphosphate binding domain (PF00456; HMM-score: 466.1)
    Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 135.1)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 38.5)
    THDP-binding (CL0254) DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 25.3)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 17.7)
    E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 14.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Ca2+, Co2+, Mg2+, Mn2+, thiamine diphosphate
  • effectors:
  • protein partners:
    SACOL_RS03050DNA-directed RNA polymerase subunit beta  [1] (data from MRSA252)
    SACOL_RS03080elongation factor Tu  [1] (data from MRSA252)
    SACOL_RS03755LysR family transcriptional regulator  [1] (data from MRSA252)
    SACOL_RS07385dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SACOL_RS08905isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    SACOL_RS1169530S ribosomal protein S5  [1] (data from MRSA252)
    SACOL_RS1176550S ribosomal protein L2  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.146
    • Ymax_pos: 25
    • Cmax: 0.17
    • Cmax_pos: 25
    • Smax: 0.185
    • Smax_pos: 23
    • Smean: 0.119
    • D: 0.135
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446403588 NCBI
  • RefSeq: WP_000481443 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWTRHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHPEYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLMEGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKDGNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFENYGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISGKLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDATDYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIMGLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALESESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVEAAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVGTAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07010 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]