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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1254 [new locus tag: NWMN_RS07080 ]
  • pan locus tag?: SAUPAN003717000
  • symbol: tkt
  • pan gene symbol?: tkt
  • synonym:
  • product: transketolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1254 [new locus tag: NWMN_RS07080 ]
  • symbol: tkt
  • product: transketolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1376509..1378497
  • length: 1989
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTTAATGAAAAAGATCAATTAGCTGTTGATACGCTACGTGCACTAAGTATCGACACA
    ATCGAAAAAGCGAATTCTGGTCATCCAGGATTACCTATGGGAGCTGCCCCAATGGCTTAC
    ACTTTGTGGACACGTCATCTGAATTTTAATCCACAATCTAAAGATTACTTCAATAGAGAC
    CGTTTCGTATTATCTGCAGGGCATGGTTCAGCATTATTGTATAGCTTGTTACATGTTTCT
    GGTAGTTTAGAATTAGAAGAATTAAAGCAATTTAGACAATGGGGTTCTAAAACACCAGGT
    CATCCTGAATACAGACATACAGATGGTGTAGAAGTTACTACCGGACCACTTGGACAAGGT
    TTTGCTATGTCAGTAGGATTAGCTTTAGCAGAAGATCACCTAGCAGGGAAATTTAATAAA
    GAAGGATATAATGTTGTAGATCATTACACATATGTATTAGCTTCTGACGGTGATTTAATG
    GAAGGTATATCGCATGAAGCAGCTTCATTTGCTGGACATAATAAATTAAGTAAATTAGTT
    GTTTTATACGATTCAAATGATATTTCATTAGATGGCGAATTAAACAAAGCTTTTTCTGAA
    AACACAAAAGCTCGTTTTGAAGCATATGGTTGGAATTACTTACTAGTTAAAGATGGTAAT
    GATTTAGAAGAAATTGATAAAGCGATTACTACAGCTAAATCTCAAGAAGGACCAACGATT
    ATTGAAGTTAAAACAACAATCGGATTTGGTTCACCGAATAAAGCAGGAACTAATGGTGTT
    CATGGGGCACCTTTAGGTGAAGTTGAAAGAAAATTAACATTCGAAAATTACGGTTTAGAT
    CCTGAAAAACGTTTTAATGTTTCAGAAGAGGTATACGAAATTTTCCAAAATACTATGTTA
    AAACGTGCTAATGAAGATGAATCTCAATGGAATTCATTATTAGAAAAATATGCAGAAACA
    TATCCTGAATTAGCAGAAGAATTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTAT
    AAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGCTGATTCTGGT
    ACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTT
    GCTGGTTCAAACAAATCCAATGTAAATGATGCAACTGATTATAGTTCTGAAACACCTGAA
    GGTAAAAATGTGTGGTTTGGTGTACGTGAATTTGCTATGGGTGCTGCTGTAAATGGTATG
    GCTGCACATGGAGGTTTACATCCATATGGTGCAACATTCTTCGTATTTAGTGATAATTTA
    AAACCAGCGTTACGTTTATCATCAATTATGGGATTAAATGCACGTTTCATCTTCACACAT
    GATTCAATTGCAGTAGGTGAAGATGGTCCTACTCATGAACCAATTGAGCAATTAGCTGGA
    TTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTA
    GCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAA
    AACTTACCGGTATTAGATGTACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCC
    TATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAA
    GTTAGTCTTGCAGTTGAAGCTGCTAAAGATCTTGAAAAACAAGGTAAATCAGTACGTGTT
    GTTTCAATGCCTAACTGGAATGCATTTGAACAACAATCTGAAGAATATAAAGAATCAGTT
    ATTCCATCAAGCGTAACAAAACGTGTTGCGATTGAAATGGCTTCACCGCTTGGATGGCAT
    AAATATGTAGGTACTGCAGGTAAAGTTATTGCTATTGACGGCTTTGGCGCAAGTGCACCT
    GGCGATTTAGTAGTTGAAAAATATGGATTTACAAAAGAAAATATCTTAAACCAAGTTATG
    AGCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1989

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1254 [new locus tag: NWMN_RS07080 ]
  • symbol: Tkt
  • description: transketolase
  • length: 662
  • theoretical pI: 4.74001
  • theoretical MW: 72256.3
  • GRAVY: -0.34139

Function[edit source | edit]

  • reaction:
    EC 2.2.1.1?  ExPASy
    TransketolaseSedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismPentose phosphate pathwaytransketolase (TIGR00232; EC 2.2.1.1; HMM-score: 908)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 74)
    pyruvate dehydrogenase (acetyl-transferring), homodimeric type (TIGR00759; EC 1.2.4.1; HMM-score: 34.3)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 20.8)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 17.6)
    alpha-ketoglutarate dehydrogenase (TIGR03186; HMM-score: 15.6)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPentose phosphate pathway Transketolase (EC 2.2.1.1) 
  • PFAM:
    THDP-binding (CL0254) Transketolase_N; Transketolase, thiamine diphosphate binding domain (PF00456; HMM-score: 466.1)
    Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 130.3)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 38.5)
    THDP-binding (CL0254) DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 25.3)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 17.7)
    E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 14.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Ca2+, Co2+, Mg2+, Mn2+, thiamine diphosphate
  • effectors:
  • protein partners:
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_0504(rpoB)DNA-directed RNA polymerase subunit beta  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.146
    • Ymax_pos: 25
    • Cmax: 0.17
    • Cmax_pos: 25
    • Smax: 0.185
    • Smax_pos: 23
    • Smean: 0.119
    • D: 0.135
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWTRHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHPEYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLMEGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKDGNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFENYGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISGKLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDATDYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDNLKPALRLSSIMGLNARFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALESESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVEAAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVGTAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]