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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07530 [old locus tag: SACOL1478 ]
  • pan locus tag?: SAUPAN003893000
  • symbol: SACOL_RS07530
  • pan gene symbol?: ald1
  • synonym:
  • product: alanine dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07530 [old locus tag: SACOL1478 ]
  • symbol: SACOL_RS07530
  • product: alanine dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1515797..1516915
  • length: 1119
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1515797..1516915) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTTAGTTGCAGTAGTCAAAGAATTAAAACAAGGTGAAGGACGTGTAGCTTGCACACCC
    GAAAATGTGCGTAAGTTAACGGATGCAGGACATAAAGTAATTGTTGAAAAAAATGCTGGC
    ATTGGTTCAGGATTTTCTAACGATATGTATGAAAAAGAAGGCGCTAAGATCGTAACTCAC
    GAACAAGCATGGGAAGCTGATCTTGTTATCAAAGTAAAAGAACCTCATGAAAGCGAATAT
    CAATATTTCAAAAAGAATCAAATTATCTGGGGATTTTTACATCTAGCATCTTCAAAAGAA
    ATAGTAGAAAAAATGCAAGAAGTTGGTGTAACTGCGATTAGTGGTGAAACCATTATAAAA
    AATGGAAAAGCAGAATTATTAGCGCCAATGAGTGCTATAGCAGGTCAACGCTCAGCAATT
    ATGGGAGCTTACTACTCTGAAGCACAACATGGTGGTCAAGGTACTTTAGTGACTGGTGTA
    CATGAAAATGTGGATATACCTGGTAGTACATATGTGATTTTCGGTGGTGGAGTAGCAGCA
    ACAAATGCAGCAAATGTTGCCTTGGGACTAAATGCTAAAGTAATCATTATCGAGTTAAAC
    GATGACCGCATTAAATATCTTGAAGATATGTATGCAGAAAAAGATGTCACAGTAGTCAAA
    TCAACACCAGAAAATTTAGCAGAACAAATTAAGAAAGCAGATGTATTTATTTCTACAATT
    TTAATTCCAGGTGCGAAACCGCCAAAATTGGTTACTCGTGAGATGGTTAAATCAATGAAA
    AAAGGTTCAGTATTAATCGATATAGCTATTGACCAAGGTGGAACTATTGAAACAATTAGA
    CCAACTACAATTTCTGATCCAGTGTATGAAGAAGAAGGTGTGATTCATTATGGTGTACCA
    AATCAACCAGGAGCAGTCCCAAGAACTTCAACAATGGCATTAGCACAAGGAAATATTGAT
    TATATATTAGAAATTTGTGACAAAGGCTTAGAACAAGCAATTAAAGATAATGAAGCCTTA
    AGTACTGGTGTAAACATTTACCAAGGACAAGTGACAAATCAAGGATTAGCTTCATCACAT
    GACCTAGATTATAAAGAAATATTAAATGTTATCGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1119

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07530 [old locus tag: SACOL1478 ]
  • symbol: SACOL_RS07530
  • description: alanine dehydrogenase
  • length: 372
  • theoretical pI: 4.89305
  • theoretical MW: 40234.7
  • GRAVY: -0.140323

Function[edit source | edit]

  • reaction:
    EC 1.4.1.1?  ExPASy
    Alanine dehydrogenaseL-alanine + H2O + NAD+ = pyruvate + NH3 + NADH
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesalanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 395.5)
    MetabolismEnergy metabolismElectron transportNAD(P)(+) transhydrogenase (AB-specific), alpha subunit (TIGR00561; EC 1.6.1.2; HMM-score: 141.1)
    Cellular processesCellular processesSporulation and germinationdipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 23.2)
    MetabolismEnergy metabolismAmino acids and aminesadenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 18.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminglutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 16.6)
    methylmalonyl-CoA mutase C-terminal domain (TIGR00640; HMM-score: 15.4)
    MetabolismAmino acid biosynthesisAromatic amino acid familyshikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 15)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 254.1)
    Form_Glyc_dh (CL0325) AlaDh_PNT_N; Alanine dehydrogenase/PNT, N-terminal domain (PF05222; HMM-score: 142.1)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 25.8)
    THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 24.5)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 22.7)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 20.3)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 17.2)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 14.9)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.2)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 14.1)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 13.9)
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.153
    • Ymax_pos: 18
    • Cmax: 0.129
    • Cmax_pos: 18
    • Smax: 0.23
    • Smax_pos: 10
    • Smean: 0.18
    • D: 0.163
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446882168 NCBI
  • RefSeq: WP_000959424 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSGFSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIVEKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGVHENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTVVKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTIETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAIKDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07530 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]