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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07820 [old locus tag: SACOL1534 ]
  • pan locus tag?: SAUPAN004024000
  • symbol: SACOL_RS07820
  • pan gene symbol?: srrB
  • synonym:
  • product: two-component sensor histidine kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07820 [old locus tag: SACOL1534 ]
  • symbol: SACOL_RS07820
  • product: two-component sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1572353..1574104
  • length: 1752
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1572353..1574104) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGAGCCGGCTAAATAGTGTCGTAATTAAACTGTGGTTAACTATTATTTTAATAGTG
    ACGACAGTTTTAATTTTATTAAGTATTGCTTTAATTACCTTTATGCAATACTATTTCACA
    CAAGAAACCGAAAATGCCATAAGAGAAGATGCTAGACGTATAAGTTCACTGGTCGAACAA
    TCACATAATAAAGAAGAAGCAATAAAATATAGTCAAACATTAATTGAAAATCCTGGTGGG
    TTGATGATTATAAATAATAAACATCGTCAATCAACGGCTTCACTTTCTAATATTAAAAAG
    CAAATGTTGAATGAAGTAGTCAACAACGACCATTTTGACGATGTGTTTGATAAAGGTAAA
    TCTGTTACTCGAAATGTAACGATTAAAGAAAAGGGCTCATCTCAAACATATATTTTGTTA
    GGCTATCCAACAAAAGCACAGAAGAATAGTCATAGCAAATATAGTGGAGTCTTTATATAT
    AAAGACTTGAAATCAATCGAAGATACAAATAATGCTATTACGATTATCACCATAATTACG
    GCTGTTATTTTCTTAACAATTACAACAGTCTTTGCGTTTTTCTTATCGTCAAGAATTACA
    AAACCTTTAAGACGTTTAAGAGACCAAGCTACACGTGTATCTGAAGGGGATTACTCTTAT
    AAACCTTCTGTCACAACGAAAGATGAAATTGGTCAATTATCGCAGGCATTTAATCAGATG
    AGTACAGAAATCGAAGAGCATGTCGACGCATTATCCACATCTAAAAATATTAGAGACAGC
    TTAATTAACTCTATGGTAGAAGGTGTCCTAGGTATTAATGAGAGTCGACAAATTATCTTA
    TCTAATAAGATGGCGAATGATATTATGGACAATATTGATGAAGATGCTAAAGCTTTCTTA
    TTAAGACAAATAGAAGATACTTTTAAATCAAAACAAACTGAAATGCGCGATTTAGAAATG
    AATGCACGATTCTTTGTTGTGACCACAAGCTATATCGACAAGATTGAACAGGGAGGTAAA
    AGTGGTGTTGTTGTGACAGTTCGTGATATGACTAATGAGCACAATCTAGATCAAATGAAG
    AAAGATTTCATTGCTAATGTATCACATGAATTACGTACACCGATATCATTACTTCAAGGT
    TATACTGAATCAATTGTAGATGGTATTGTTACAGAACCGGATGAAATAAAAGAATCGCTT
    GCCATTGTCCTTGATGAATCGAAACGTTTAAATCGTTTAGTTAATGAATTGTTAAATGTC
    GCACGCATGGATGCTGAAGGGTTATCCGTAAATAAAGAAGTTCAGCCTATTGCAGCGTTA
    CTAGATAAGATGAAAATTAAGTATCGCCAACAAGCTGATGATTTAGGTCTAAATATGACT
    TTTAATTATTGTAAGAAGCGTGTTTGGAGTTATGATATGGATCGCATGGACCAAGTACTA
    ACGAACTTAATTGATAATGCATCACGTTATACGAAACCTGGAGATGAAATTGCAATTACT
    TGTGATGAAAATGAAAGCGAAGATATTTTATACATTAAAGATACAGGTACAGGCATTGCA
    CCAGAACATTTACAACAAGTATTTGATCGTTTTTATAAAGTTGATGCAGCGAGAACGCGA
    GGTAAACAAGGTACCGGTTTAGGTTTGTTCATTTGTAAAATGATTATCGAAGAGCATGGT
    GGTTCCATAGATGTTAAAAGCGAATTAGGGAAAGGCACAACATTTATTATTAAACTACCA
    AAACCAGAATAA
    60
    120
    180
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    360
    420
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    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1752

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07820 [old locus tag: SACOL1534 ]
  • symbol: SACOL_RS07820
  • description: two-component sensor histidine kinase
  • length: 583
  • theoretical pI: 5.76595
  • theoretical MW: 66075.2
  • GRAVY: -0.3

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 242.7)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 199.2)
    Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 135)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 95.8)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.6)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.6)
    MetabolismCentral intermediary metabolismNitrogen fixationnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.6)
    Signal transductionRegulatory functionsProtein interactionsnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.6)
  • TheSEED:
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 95.1)
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 64.9)
    no clan definedHAMP; HAMP domain (PF00672; HMM-score: 62.7)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 23.4)
    no clan definedHalogen_Hydrol; 5-bromo-4-chloroindolyl phosphate hydrolysis protein (PF10112; HMM-score: 18.9)
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.8)
    PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 16.8)
    no clan definedCep57_MT_bd; Centrosome microtubule-binding domain of Cep57 (PF06657; HMM-score: 14.5)
    EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 13.6)
    Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 12.6)
    DUF3328; Domain of unknown function (DUF3328) (PF11807; HMM-score: 12.3)
    Vpu; Vpu protein (PF00558; HMM-score: 12.3)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 10.8)
    no clan definedSerinc; Serine incorporator (Serinc) (PF03348; HMM-score: 9)
    PspB; Phage shock protein B (PF06667; HMM-score: 7.9)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 7.6)
    no clan definedTRP; Transient receptor potential (TRP) ion channel (PF06011; HMM-score: 7.2)
    CemA; CemA family (PF03040; HMM-score: 7.1)
    DUF3165; Protein of unknown function (DUF3165) (PF11364; HMM-score: 7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.206
    • Ymax_pos: 12
    • Cmax: 0.165
    • Cmax_pos: 41
    • Smax: 0.513
    • Smax_pos: 7
    • Smean: 0.412
    • D: 0.286
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

  • GI: 446910513 NCBI
  • RefSeq: WP_000987769 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETENAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQMLNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIYKDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDYSYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESRQIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDKIEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTEPDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQQADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESEDILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVKSELGKGTTFIIKLPKPE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07820 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]