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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07955 [old locus tag: SACOL1562 ]
  • pan locus tag?: SAUPAN004072000
  • symbol: SACOL_RS07955
  • pan gene symbol?: bfmBAA
  • synonym:
  • product: 2-oxoisovalerate dehydrogenase subunit alpha

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07955 [old locus tag: SACOL1562 ]
  • symbol: SACOL_RS07955
  • product: 2-oxoisovalerate dehydrogenase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 1597526..1598518
  • length: 993
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1597526..1598518) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    481
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    901
    961
    ATGATTGATTATAAATCATTAGGCCTTAGCGAAGAAGACCTAAAAGTAATATATAAATGG
    ATGGATTTAGGTAGAAAAATAGATGAAAGACTGTGGTTATTGAACCGTGCAGGTAAAATT
    CCATTTGTTGTAAGTGGTCAAGGACAAGAAGCAACTCAGATTGGGATGGCATACGCATTA
    GAAGAAGGTGATATTACAGCGCCTTATTATAGAGATCTAGCTTTCGTTACTTATATGGGT
    ATCTCAGCGTATGATACGTTTTTATCAGCGTTTGGAAAGAAAGACGATGTTAACTCTGGT
    GGAAAACAAATGCCATCACACTTTAGTAGTAGAGCTAAGAATATTTTGTCTCAAAGTTCA
    CCGGTAGCAACTCAAATACCCCATGCTGTTGGTGCTGCTTTAGCTTTAAAAATGGATGGT
    AAGAAGAAAATTGCAACAGCAACTGTTGGTGAAGGTAGCTCAAACCAAGGTGACTTCCAT
    GAAGGTTTAAACTTCGCGGGTGTACACAAACTTCCTTTTGTATGTGTCATTATAAATAAT
    AAATATGCAATTTCAGTACCTGATTCATTACAATACGCAGCTGAAAAATTATCGGATAGA
    GCACTTGGTTACGGTATACATGGTGAACAAGTAGATGGTAATGATCCATTAGCAATGTAT
    AAAGCGATGAAAGAAGCAAGAGATCGAGCTATTTCTGGTCAAGGTTCAACTTTGATTGAA
    GCAGTAACAAGTCGTATGACAGCTCATTCATCAGATGATGACGACCAATATCGTACGAAA
    GAAGAGCGTGAAACGCTTAAAAAAGCAGACTGCAATGAAAAGTTCAAAAAAGAGTTGCTT
    TCAGCTGGCATTATCGATGATGCTTGGTTGGCAGAAATAGAAGCGGAGCATAAAGACATC
    ATCAATAAAGCGACAAAGGCTGCTGAAGACGCGCCATATCCAAGTGTCGAAGAAGCCTAT
    GCATTTGTGTACGAAGAAGGGAGTCTTAACTAA
    60
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    993

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07955 [old locus tag: SACOL1562 ]
  • symbol: SACOL_RS07955
  • description: 2-oxoisovalerate dehydrogenase subunit alpha
  • length: 330
  • theoretical pI: 4.94909
  • theoretical MW: 36229.5
  • GRAVY: -0.399091

Function[edit source | edit]

  • reaction:
    EC 1.2.4.1?  ExPASy
    Pyruvate dehydrogenase (acetyl-transferring)Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2
  • TIGRFAM:
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 316.8)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 223.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
    MetabolismEnergy metabolismTCA cycleoxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 10.9)
  • TheSEED:
  • PFAM:
    THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 282.4)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 24)
    DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 21.7)
    XFP_N; XFP N-terminal domain (PF09364; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 60
    • Cmax: 0.215
    • Cmax_pos: 60
    • Smax: 0.146
    • Smax_pos: 47
    • Smean: 0.07
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446490499 NCBI
  • RefSeq: WP_000568353 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVVSGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGKQMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFHEGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLAMYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFKKELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07955 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]