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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08345 [old locus tag: SACOL1637 ]
  • pan locus tag?: SAUPAN004163000
  • symbol: SACOL_RS08345
  • pan gene symbol?: dnaK
  • synonym:
  • product: molecular chaperone DnaK

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08345 [old locus tag: SACOL1637 ]
  • symbol: SACOL_RS08345
  • product: molecular chaperone DnaK
  • replicon: chromosome
  • strand: -
  • coordinates: 1666574..1668406
  • length: 1833
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1666574..1668406) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
    1681
    1741
    1801
    ATGAGTAAAATTATTGGTATAGACTTAGGTACAACAAATTCATGTGTAACAGTATTAGAA
    GGCGATGAGCCAAAAGTAATTCAAAACCCTGAAGGTTCACGTACAACACCATCTGTTGTA
    GCTTTCAAAAATGGAGAAACTCAAGTTGGTGAAGTAGCAAAACGTCAAGCTATTACAAAC
    CCAAACACTGTTCAATCTATTAAACGTCATATGGGTACTGATTATAAAGTAGATATTGAA
    GGTAAATCATACACACCACAAGAAATCTCAGCTATGATTTTACAAAACTTAAAAAATACA
    GCTGAAAGCTATTTAGGTGAGAAAGTTGACAAAGCTGTAATTACAGTACCTGCATACTTT
    AACGATGCTGAACGTCAAGCAACTAAAGATGCTGGTAAAATTGCTGGTTTAGAAGTTGAG
    CGTATCATTAATGAACCAACAGCTGCAGCATTAGCATATGGTTTAGACAAAACTGATAAA
    GATGAAAAAGTTCTTGTTTTTGACTTAGGTGGCGGTACATTTGACGTATCTATCCTAGAA
    TTAGGTGACGGTGTATTCGAAGTACTATCAACAGCCGGTGACAACAAACTTGGCGGTGAT
    GATTTTGACCAAGTAATTATTGACTACCTAGTTGCAGAATTCAAAAAAGAAAATGGCGTA
    GACTTATCTCAAGATAAAATGGCATTACAACGTTTGAAAGATGCTGCTGAAAAAGCTAAA
    AAAGACTTATCAGGTGTATCACAAACTCAAATCTCATTACCATTTATCTCAGCTGGTGAA
    AACGGTCCATTACACTTAGAAGTAAACTTAACTCGTTCTAAATTTGAAGAATTATCAGAT
    TCATTAATTAGAAGAACAATGGAACCTACACGCCAAGCAATGAAAGACGCTGGCTTAACA
    AACTCAGATATCGATGAAGTTATCTTAGTTGGTGGATCAACTCGTATTCCAGCAGTACAA
    GAAGCTGTCAAAAAAGAAATCGGTAAAGAGCCTAACAAAGGAGTAAACCCGGACGAAGTA
    GTGGCAATGGGAGCTGCAATCCAAGGTGGCGTTATCACAGGTGACGTTAAAGACGTAGTA
    TTATTAGACGTAACACCACTATCTTTAGGTATTGAAATTTTAGGTGGACGTATGAATACG
    TTAATTGAACGTAACACTACGATTCCTACATCTAAATCTCAAATCTATTCAACAGCAGTA
    GATAATCAACCATCAGTAGATGTACACGTATTACAAGGTGAACGTCCAATGGCTGCGGAT
    AATAAAACACTTGGTAGATTCCAATTGACTGATATTCCACCAGCTGAACGTGGTAAACCT
    CAAATTGAAGTAACGTTTGATATCGATAAAAACGGTATTGTAAATGTAACTGCAAAAGAC
    TTAGGTACAAATAAAGAACAAAGAATTACAATTCAATCAAGTTCTTCATTATCAGACGAA
    GAAATCGACCGTATGGTAAAAGATGCTGAAGTTAACGCTGAAGCAGATAAAAAACGTCGT
    GAAGAAGTAGACTTAAGAAACGAAGCTGACAGTCTAGTATTCCAAGTTGAAAAAACTTTA
    ACTGATTTAGGCGAAAATATCGGTGAAGAAGATAAAAAATCTGCTGAAGAGAAAAAAGAC
    GCTCTTAAAACTGCTTTAGAAGGTCAAGATATAGAAGATATTAAATCTAAAAAAGAAGAA
    CTTGAAAAAGTGATTCAAGAATTATCAGCAAAAGTATATGAGCAAGCGGCTCAACAGCAA
    CAACAAGCACAAGGTGCTAATGCTGGTCAAAACAATGATAGTACTGTAGAAGATGCTGAA
    TTTAAAGAAGTAAAAGACGACGACAAAAAATAA
    60
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    1560
    1620
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    1740
    1800
    1833

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08345 [old locus tag: SACOL1637 ]
  • symbol: SACOL_RS08345
  • description: molecular chaperone DnaK
  • length: 610
  • theoretical pI: 4.38499
  • theoretical MW: 66360.9
  • GRAVY: -0.523279

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein folding and stabilizationchaperone protein DnaK (TIGR02350; HMM-score: 938.7)
    Genetic information processingProtein fateProtein folding and stabilizationFe-S protein assembly chaperone HscA (TIGR01991; HMM-score: 574.4)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycancell shape determining protein, MreB/Mrl family (TIGR00904; HMM-score: 66.9)
    ethanolamine utilization protein EutJ family protein (TIGR02529; HMM-score: 59.7)
    MetabolismCentral intermediary metabolismPhosphorus compoundsexopolyphosphatase (TIGR03706; EC 3.6.1.11; HMM-score: 13)
  • TheSEED:
  • PFAM:
    Actin_ATPase (CL0108) HSP70; Hsp70 protein (PF00012; HMM-score: 822.8)
    MreB_Mbl; MreB/Mbl protein (PF06723; HMM-score: 72.4)
    Hydantoinase_A; Hydantoinase/oxoprolinase (PF01968; HMM-score: 16.2)
    StbA; StbA protein (PF06406; HMM-score: 15.7)
    FtsA; Cell division protein FtsA (PF14450; HMM-score: 10.5)
    no clan definedDUF775; Protein of unknown function (DUF775) (PF05603; HMM-score: 7.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL_RS08350nucleotide exchange factor GrpE  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.175
    • Ymax_pos: 22
    • Cmax: 0.13
    • Cmax_pos: 22
    • Smax: 0.321
    • Smax_pos: 16
    • Smean: 0.227
    • D: 0.195
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445956861 NCBI
  • RefSeq: WP_000034716 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKNGETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAESYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDKDEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKGVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDKNGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNEADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08345 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]