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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10285 [old locus tag: SACOL1967 ]
  • pan locus tag?: SAUPAN004935000
  • symbol: SACOL_RS10285
  • pan gene symbol?: pcrB
  • synonym:
  • product: geranylgeranylglyceryl/heptaprenylglyceryl phosphate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS10285 [old locus tag: SACOL1967 ]
  • symbol: SACOL_RS10285
  • product: geranylgeranylglyceryl/heptaprenylglyceryl phosphate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2030888..2031580
  • length: 693
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2030888..2031580) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTATGACATTAAAAAATGGCGCCATATTTTTAAATTAGACCCAGCTAAACATATTTCA
    GATGATGATTTAGATGCGATTTGTATGTCTCAAACAGATGCAATTATGATTGGTGGAACT
    GATGACGTTACTGAAGATAATGTCATTCATTTAATGAGCAGAGTAAGAAGATACCCATTG
    CCTTTAGTACTTGAAATATCAAACATCGAAAGTGTAATGCCTGGTTTTGATTTTTATTTT
    GTACCTACAGTACTGAACAGTACAGATGTTGTATTTCACAATGGTACATTATTAGAAGCG
    CTTAAAACATATGGACATAGTATAGATTTTGAGGAAGTAATATTTGAAGGGTATGTCGTG
    TGCAATGCTGATAGCAAAGTGGCAAAACATACCAAAGCAAATACAGATTTAACAACAGAA
    GATTTAGAAGCATATGCCCAAATGGTCAATCATATGTATCGATTACCGGTTATGTATATA
    GAGTATAGTGGCATTTATGGCGACGTATCAAAGGTTCAAGCTGTCTCAGAACATCTAACA
    GAAACGCAACTTTTTTATGGTGGCGGTATTTCCTCAGAACAACAAGCGACAGAGATGGCA
    GCTATTGCAGATACAATTATCGTCGGTGATATTATTTATAAAGATATTAAAAAAGCTTTA
    AAAACAGTAAAAATAAAGGAGTCTAGTAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS10285 [old locus tag: SACOL1967 ]
  • symbol: SACOL_RS10285
  • description: geranylgeranylglyceryl/heptaprenylglyceryl phosphate synthase
  • length: 230
  • theoretical pI: 4.67385
  • theoretical MW: 25888.4
  • GRAVY: -0.0947826

Function[edit source | edit]

  • reaction:
    EC 2.5.1.n9?  ExPASy
    Heptaprenylglyceryl phosphate synthaseAll-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(all-trans-heptaprenyl)glycerol 1-phosphate
  • TIGRFAM:
    phosphoglycerol geranylgeranyltransferase (TIGR04146; EC 2.5.1.41; HMM-score: 245.3)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 204.4)
    phosphoglycerol geranylgeranyltransferase, putative (TIGR04147; EC 2.5.1.41; HMM-score: 187.6)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 46.9)
    HAD superfamily (subfamily IG) hydrolase, 5'-nucleotidase (TIGR02244; HMM-score: 17.3)
  • TheSEED:
  • PFAM:
    TIM_barrel (CL0036) PcrB; PcrB family (PF01884; HMM-score: 233)
    Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 21.7)
    HAD (CL0137) 5_nucleotid; 5' nucleotidase family (PF05761; HMM-score: 13.8)
    TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 13.3)
    no clan definedPox_H7; Late protein H7 (PF04787; HMM-score: 12.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.093
    • Ymax_pos: 70
    • Cmax: 0.109
    • Cmax_pos: 46
    • Smax: 0.136
    • Smax_pos: 2
    • Smean: 0.065
    • D: 0.082
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446194215 NCBI
  • RefSeq: WP_000272070 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVTEDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALKTYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYIEYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKKALKTVKIKESSK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS10285 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]