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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS12090 [old locus tag: SACOL2298 ]
  • pan locus tag?: SAUPAN005782000
  • symbol: SACOL_RS12090
  • pan gene symbol?:
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS12090 [old locus tag: SACOL2298 ]
  • symbol: SACOL_RS12090
  • product: N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: -
  • coordinates: 2359394..2360032
  • length: 639
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2359394..2360032) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCAACAAGGGAAAGGTATTGTACAGACAAAAGAAGAGGATGGTAAATTTGTTGAAGCA
    AATAATAATGAAATTGCTAAAGCAATGACTATTTCACATAAAGACAATGATATGAAGTAT
    ATGGATATCACAGAAAAAGTGCCAATGTCGGAATCTGAAGTTAACCAATTGCTAAAAGGT
    AAGGGGATTTTAGAAAATCGAGGGAAAGTTTTTCTAGAAGCTCAAGAAAAATATGAGGTT
    AATGTCATTTATCTTGTTAGCCATGCATTAGTAGAAACAGGTAACGGCAAATCAGAATTA
    GCAAAAGGCATTAAAGATGGGAAAAAACGCTATTACAACTTTTTTGGTATAGGAGCATTC
    GATAGTAGTGCTGTTCGTAGTGGGAAAAGTTATGCTGAAAAGGAACAATGGACATCACCA
    GATAAGGCGATTATTGGTGGTGCAAAGTTCATTCGTAATGAATATTTTGAAAACAATCAA
    CTGAATTTATATCAAATGCGATGGAATCCAGAAAATCCTGCGCAACATCAATATGCGAGT
    GACATTCGCTGGGCAGATAAAATTGCCAAATTAATGGATAAATCCTATAAGCAGTTTGGT
    ATAAAGAAAGATGATATTAGACAAACATATTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    639

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS12090 [old locus tag: SACOL2298 ]
  • symbol: SACOL_RS12090
  • description: N-acetylmuramoyl-L-alanine amidase
  • length: 212
  • theoretical pI: 9.4162
  • theoretical MW: 24389.4
  • GRAVY: -0.851887

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA-3-methyladenine glycosylase I (TIGR00624; EC 3.2.2.20; HMM-score: 12)
  • TheSEED:
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 64.3)
    no clan definedDcc1; Sister chromatid cohesion protein Dcc1 (PF09724; HMM-score: 17)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 29
    • Cmax: 0.162
    • Cmax_pos: 29
    • Smax: 0.149
    • Smax_pos: 7
    • Smean: 0.09
    • D: 0.104
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_011447058 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQLLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNFFGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPENPAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS12090 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]