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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13510 [old locus tag: SACOL2579 ]
  • pan locus tag?: SAUPAN006228000
  • symbol: SACOL_RS13510
  • pan gene symbol?: crtP
  • synonym:
  • product: diapolycopene oxygenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS13510 [old locus tag: SACOL2579 ]
  • symbol: SACOL_RS13510
  • product: diapolycopene oxygenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2642387..2643880
  • length: 1494
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2642387..2643880) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    1441
    ATGACTAAACATATCATCGTTATTGGTGGTGGCTTAGGTGGGATTTCTGCAGCAATTCGA
    ATGGCACAAAGTGGCTATTCGGTCTCATTATATGAACAAAATAATCATATAGGAGGCAAA
    GTGAATCGTCATGAATCAGATGGCTTTGGCTTTGATTTAGGTCCATCTATTTTAACGATG
    CCTTATATTTTTGAAAAATTATTCGAATATAGCAAGAAGCAAATGTCAGACTACGTTACA
    ATCAAGCGATTGCCACATCAATGGCGTAGCTTTTTTCCAGATGGAACGACTATCGATTTG
    TATGAAGGTATTAAAGAAACAGGTCAGCATAATGCGATATTGTCGAAACAGGATATAGAG
    GAACTGCAAAATTATTTGAATTATACAAGACGAATCGATCGTATTACTGAAAAAGGGTAT
    TTCAACTATGGTTTAGATACACTATCTCAAATTATTAAATTTCATGGGCCATTAAATGCT
    CTTATTAATTATGATTATGTACATACTATGCAACAGGCCATAGACAAGCGTATCTCGAAT
    CCATACTTGCGACAAATGTTAGGCTATTTTATCAAATATGTAGGTTCTTCATCATACGAT
    GCGCCAGCTGTATTATCTATGTTATTCCATATGCAACAAGAGCAAGGCCTTTGGTATGTA
    GAAGGTGGAATCCATCATTTAGCCAATGCCTTGGAAAAGCTAGCGCGTGAAGAAGGTGTC
    ACAATTCATACAGGTGCACGTGTGGACAATATTAAAACATATCAAAGACGTGTGACGGGT
    GTCAGATTAGATACAGGTGAGTTTGTAAAGGCAGATTATATTATTTCAAATATGGAAGTC
    ATACCTACTTATAAATATTTAATTCACCTTGATACTCAACGATTAAACAAATTAGAGAGG
    GAATTTGAGCCGGCAAGCTCAGGATATGTGATGCATTTAGGTGTTGCTTGCCAATACCCG
    CAATTAGCACATCATAATTTCTTTTTTACGGAAAATGCTTATCTCAATTATCAACAAGTT
    TTTCATGAAAAGGTATTGCCAGATGATCCGACCATTTATCTAGTAAATACGAATAAAACT
    GATCACACACAAGCGCCAGTAGGTTATGAAAATATCAAAGTCTTACCACATATTCCATAT
    ATTCAAGATCAGCCTTTTACCACTGAAGATTATGCGAAGTTTAGGGATAAAATTTTGGAT
    AAATTAGAAAAAATGGGACTTACTGATTTAAGAAAACACATTATTTATGAAGATGTTTGG
    ACACCGGAGGATATTGAAAAAAATTATCGTTCTAATCGTGGTGCAATATATGGTGTTGTA
    GCAGATAAAAAGAAAAACAAAGGATTTAAATTTCCTAAAGAAAGTCAGTATTTTGAAAAC
    TTGTACTTTGTAGGTGGATCAGTAAATCCTGGTGGTGGCATGCCAATGGTTACATTAAGT
    GGGCAACAAGTCGCAGACAAAATAAACGCGCGAGAAGCGAAGAATAGGAAGTGA
    60
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    1494

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS13510 [old locus tag: SACOL2579 ]
  • symbol: SACOL_RS13510
  • description: diapolycopene oxygenase
  • length: 497
  • theoretical pI: 7.93809
  • theoretical MW: 57186.6
  • GRAVY: -0.491549

Function[edit source | edit]

  • reaction:
    EC 1.14.99.44?  ExPASy
    Diapolycopene oxygenase4,4'-diapolycopene + 4 AH2 + 4 O2 = 4,4'-diapolycopenedial + 4 A + 6 H2O
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherphytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 330.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercarotene isomerase (TIGR02730; EC 5.-.-.-; HMM-score: 96.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherC-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 87.6)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 39.9)
    Unknown functionEnzymes of unknown specificityflavoprotein, HI0933 family (TIGR00275; HMM-score: 36.7)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 34.7)
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 33.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprotoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 30.9)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 30.1)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 28.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamineglycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 25.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin 3B synthase CobZ (TIGR02485; HMM-score: 22.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA:m(5)U-54 methyltransferase (TIGR00137; EC 2.1.1.74; HMM-score: 22.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 20.7)
    MetabolismEnergy metabolismElectron transportflavocytochrome c (TIGR01813; HMM-score: 19)
    Cellular processesCellular processesDetoxificationalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 18.3)
    Cellular processesCellular processesAdaptations to atypical conditionsalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 18.3)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 17.2)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 16.1)
    MetabolismEnergy metabolismElectron transportglutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 15.7)
    Cellular processesCellular processesDetoxificationmercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 15.7)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 15.5)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesUDP-galactopyranose mutase (TIGR00031; EC 5.4.99.9; HMM-score: 15)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 14.8)
    MetabolismEnergy metabolismTCA cyclesuccinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01811; EC 1.3.99.1; HMM-score: 14.7)
    MetabolismEnergy metabolismElectron transportthioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 14.1)
    MetabolismAmino acid biosynthesisGlutamate familyglutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 14)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 13.9)
    MetabolismEnergy metabolismAnaerobicglycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 13.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis enzyme (TIGR00292; HMM-score: 13.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherphytoene desaturase (TIGR02731; EC 1.14.99.-; HMM-score: 13.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 13.1)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, monomeric form (TIGR01377; HMM-score: 12.8)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 12.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 12.2)
    Unknown functionEnzymes of unknown specificityGMC family mycofactocin-associated oxidreductase (TIGR04542; EC 1.-.-.-; HMM-score: 12.1)
    lycopene cyclase family protein (TIGR01790; HMM-score: 11.7)
    Unknown functionEnzymes of unknown specificityputative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 11.5)
    MetabolismCentral intermediary metabolismNitrogen metabolismnitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 11)
    MetabolismAmino acid biosynthesisOtherpyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 10.9)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 10.8)
    MetabolismEnergy metabolismElectron transportglutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 10.3)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 10.1)
    MetabolismEnergy metabolismAnaerobicglycerol-3-phosphate dehydrogenase, anaerobic, A subunit (TIGR03377; EC 1.1.5.3; HMM-score: 9.1)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 91.3)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 53.7)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 43.8)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 42.8)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 36.5)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 36.2)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 35.1)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 34.2)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 30.5)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 17.3)
    Trp_halogenase; Tryptophan halogenase (PF04820; HMM-score: 13.8)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 13.4)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 12.6)
    Thi4; Thi4 family (PF01946; HMM-score: 12.3)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 12.1)
    MCRA; MCRA family (PF06100; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.188
    • Ymax_pos: 25
    • Cmax: 0.144
    • Cmax_pos: 25
    • Smax: 0.376
    • Smax_pos: 20
    • Smean: 0.218
    • D: 0.2
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446082601 NCBI
  • RefSeq: WP_000160456 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRHESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYEGIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNALINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLWYVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIISNMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAYLNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYAKFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFKFPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS13510 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]