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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13960 [old locus tag: SACOL2665 ]
  • pan locus tag?: SAUPAN006374000
  • symbol: SACOL_RS13960
  • pan gene symbol?:
  • synonym:
  • product: phage infection protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS13960 [old locus tag: SACOL2665 ]
  • symbol: SACOL_RS13960
  • product: phage infection protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2729253..2732234
  • length: 2982
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2729253..2732234) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGAACGCATTTAAATTATTTAAAATGGATCTGAAGAAAGTAGCTAAGACGCCAGCT
    GTGTGGATTATCTTAGCAGGCTTAGCTATTTTGCCATCGTTCTACGCTTGGTTTAACTTA
    TGGGCAATGTGGGATCCATATGGCAACACGGGACACATCAAGGTCGCAGTCGTTAATGAA
    GATAAAGGCGACACAATCAGAGGGAAAAAAGTTAATGTCGGTAATACGATGGTTAATACA
    CTCAAGAAAAATAAAAGTTTTGATTGGCAGTTTGTAAGTAGAGAGAAAGCTGATCATGAG
    ATAAAAATGGGTAAATATTTTGCAGGTATTTACATCCCATCTAAGTTTACACATGAAATT
    ACAGGGACACTACGTAAGCAGCCTCAAAAAGCAGATGTAGAATTTAAGGTGAATCAGAAG
    ATTAACGCTGTTGCGTCTAAGCTAACAGATACTGGTTCGTCAGTTGTCGTTGAAAAAGCG
    AATGAACAATTTAATAAAACAGTAACTCGAGCATTATTAGAAGAAGCTAACAAAGCAGGT
    TTAACTATTGAAGAAAATGTGCCGACAATTAACAAGATAAAAAATGCGGTATATTCAGCA
    GATAAAGCTTTACCTAAGATTAATGACTTTGCGAATAAAATTGTATATTTGAATAACCAC
    CAAGCGGATTTAGATAAATATGCCAATGATTTTAGAAAACTAGGAAATTATAAAGGTGAT
    ATTTTAGATGCTCAGAAAAAATTAAACGAAGTCAATGGTGCTATTCCGCAACTTAATGAA
    AAGGCTAAGTTGATATTAGCTTTAAATAATTATATGCCGAAAATTGAAAAAGCGTTAAAT
    TTTGCAGCTGATGACGTGCCAGCGCAGTTCCCTAAAATTAATCAAGGACTTAACATTGCG
    AGTCAAGGTATTGATCAAGCTAATGGACAGTTAAATGATGCCAAAGGCTTCGTCACACAA
    GTTAGAAGTAGAGTCGGTGATTATCAAGATGCAATTCGACGCGCGCAAGATTTAAATCGA
    AGAAACCAGCAACAGATTCCTCAAAATAGCGCGGCGAACAACGAAACATCAAATAGTGCA
    CCTGCAGCTGGTAATGGTGTAGCATCAACGCCACCAAGTGCACCAAGTGGCGATACTGCA
    CCAAATAATAATGTTACGCAAAATACCGCACCAAATAGTAATAATGCGCCTGTATCGACT
    ACACCACAAAGTACAAGCGGGAAAAAAGATGGTCAAAGTTTTGTAGATATAACAACAACA
    CAAGTCAGCACAGCTAACGAGAACACACAAAACATTACAGATAAAGATGTTAAATCAATG
    GAAGCGGCATTAACGGGCTCTTTATTATCATTATCAAATAATTTAGATACCCAAGCGAAA
    GCCGCACAAAAAGATAGTCAGGCATTACGTAATATTTCGTATGGGATTTTAGCATCGGAC
    AAGCCTTCTGATTTTAGAGAGTCTTTAGATAATGTTAAGTCCGGTTTAGAATACACAACG
    CAATATAATCAACAATTTATCGATACATTAAAAGAGATTGAGAAGAATGAAAATGTTGAT
    TTATCAAAAGAAATTGATAAGGTAAAAGCAGCTAATAATCGAATTAATGAATCATTAAGG
    TTAGTTAATCAATTAAGCAATGCATTAAAGAATGGTAGTTCAGGAACTGCTGAAGCTACT
    AAATTACTAGATCAACTTTCAAAACTAGATTCATCATTATCATCATTTAGAGATTATGTT
    AAAAAAGATCTTAACAGCTCTTTAGTATCAATATCACAACGTATTATGGATGAATTGAAC
    AAAGGGCAAACTGCATTATCCAATGTTCAGTCTAAATTAAATACAATTGATCAAGTCATT
    AACAGTGGACAAGCTATTTTAAAAAATGGTAAAACACGTATTGATCGCTTACAAACAGTA
    TTACCAAGTATTGAACAACAATACATTAGTGCTGTTAAAAATGCTCAAGCAAACTTCTCG
    AAAGTGAAAAGTGATGTAGCTAAAGCTGCTAACTTTGTGCGCAATGACTTACCACAGTTA
    GAACAGCGATTAACTAATGCGACAGCAAGTGTGAATAAAAATTTACCAACGTTATTAAAT
    GGTTATGATCAAGCGGTAGGATTACTAAATAAAAATCAGCCACAAGCGAAAAAGGCTTTA
    TCAGATTTAGCTGATTTTTCTCAAAATAAATTGCCTGATGTTGAAAAAGATTTGAAAAAA
    GCGAATAAAATTTTCAAGAAATTAGACAAAGATGATGCAGTCGACAAATTAATCGACACA
    CTTAAGAATGATTTGAAAAAGCAAGCGGGTATTATTGCAAATCCTATTAATAAGAAGACT
    GTTGATGTTTTCCCAGTTAAGGATTATGGTTCAGGTATGACACCATTCTATACTGCACTG
    TCAGTATGGGTAGGTGCACTCTTGATGGTAAGTTTATTAACGGTTGATAATAAACATAAG
    AGTCTAGAGTCAGTCTTAACGACAAGACAAGTGTTCTTAGGTAAGGCAGGATTCTTTATA
    ATGCTTGGTATGTTGCAAGCACTCATTGTATCGGTTGGAGATTTGTTAATCCTAAAAGCA
    GGAGTTGAGTCACCTGTATTATTTGTACTTATAACGATTTTCTGTTCGATTATTTTCAAC
    TCAATCGTATATACGTGCGTATCATTACTTGGTAACCCAGGTAAAGCCATTGCAATCGTA
    TTGCTTGTATTACAAATTGCAGGTGGTGGGGGAACATTCCCAATTCAAACTACGCCACAA
    TTTTTCCAAAACATTTCGCCATACTTACCATTTACGTATGCAATTGATTCATTACGTGAA
    ACAGTAGGCGGTATTGTTCCGGAAATCCTAATTACAAAATTAATTATATTAACGTTATTT
    GGTATAGGATTCTTCGTTGTAGGTTTAATTTTAAAACCTGTAACAGATCCATTGATGAAG
    CGCGTATCTGAAAAAGTTGACCAAAGTAACGTTACAGAATAA
    60
    120
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    720
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    840
    900
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    1020
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    2160
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    2700
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    2820
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    2940
    2982

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS13960 [old locus tag: SACOL2665 ]
  • symbol: SACOL_RS13960
  • description: phage infection protein
  • length: 993
  • theoretical pI: 9.90943
  • theoretical MW: 108715
  • GRAVY: -0.279255

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    YhgE/Pip C-terminal domain (TIGR03062; HMM-score: 167.6)
    YhgE/Pip N-terminal domain (TIGR03061; HMM-score: 158.5)
    type VII secretion protein EsaA (TIGR03929; HMM-score: 83.4)
    MetabolismTransport and binding proteinsOtherdaunorubicin resistance ABC transporter membrane protein (TIGR01247; HMM-score: 20.7)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtwin arginine-targeting protein translocase, TatA/E family (TIGR01411; HMM-score: 6.2)
  • TheSEED:
  • PFAM:
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 80.7)
    ABC2_membrane; ABC-2 type transporter (PF01061; HMM-score: 33.7)
    DUF3533; Protein of unknown function (DUF3533) (PF12051; HMM-score: 31.5)
    no clan definedADIP; Afadin- and alpha -actinin-Binding (PF11559; HMM-score: 18.9)
    6PGD_C (CL0106) 6PGD; 6-phosphogluconate dehydrogenase, C-terminal domain (PF00393; HMM-score: 16.4)
    no clan definedBaculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 15.1)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 11.2)
    no clan definedDUF842; Eukaryotic protein of unknown function (DUF842) (PF05811; HMM-score: 11)
    Traffic (CL0147) V-SNARE_C; Snare region anchored in the vesicle membrane C-terminus (PF12352; HMM-score: 10.8)
    no clan definedDUF2935; Domain of unknown function (DUF2935) (PF11155; HMM-score: 8.2)
    Traffic (CL0147) Sec20; Sec20 (PF03908; HMM-score: 8.1)
    ABC-2 (CL0181) ABC2_membrane_5; ABC-2 family transporter protein (PF13346; HMM-score: 6.8)
    STAND_N (CL0587) SesA; N-terminal domain on NACHT_NTPase and P-loop NTPases (PF17107; HMM-score: 6.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0.01
    • Extracellular Score: 0.01
    • Internal Helices: 6
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.251
    • Ymax_pos: 12
    • Cmax: 0.155
    • Cmax_pos: 37
    • Smax: 0.71
    • Smax_pos: 5
    • Smean: 0.647
    • D: 0.405
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

  • GI: 446707764 NCBI
  • RefSeq: WP_000785110 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMWDPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIKMGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKANEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLNNHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIEKALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRRAQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPNSNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLSLSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFIDTLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQLSKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQAILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQRLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKKANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYTALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLLILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFPIQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILKPVTDPLMKRVSEKVDQSNVTE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS13960 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]