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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00082
  • pan locus tag?: SAUPAN000922000
  • symbol: SAOUHSC_00082
  • pan gene symbol?: sbnH
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00082
  • symbol: SAOUHSC_00082
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 88997..90199
  • length: 1203
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCAT
    TATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCG
    AGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACA
    ATTAGTCAGTATGTTGAAGGATTCGAAGTTGCATCTCAAGGTGAAATAGCAAAAGGTCTT
    GCTTTTAAACCAGCAAATCATATTATTTTTGGTGGCCCTGGTAAGACAGACGAGGAACTA
    AGATATGCAGTAAGTGAAGGTGTTCAGCGTATTCATGTTGAAAGTATGCATGAATTACAA
    CGGCTAAATGCCATCTTAGAAGATGAAGATAAGACACAACACATTTTATTGCGTGTTAAT
    TTAGCAGGACCATTTCCCAATGCAACGTTGCATATGGCAGGACGCCCAACACAATTTGGT
    ATTTCTGAAGACGAAGTTGATGATGTCATTGAAGCTGCGCTCGCAATGCCAAAGATTCAT
    CTAGATGGATTTCATTTTCATTCTATTTCTAACAATTTAGACTCGAATTTACATGTCGAT
    GTAGTGAAACTTTATTTTAAAAAAGCAAAGGCATGGTCTGAAAAACATCGATTTCCACTC
    AAACATATCAATCTTGGTGGTGGCATAGGCGTTAACTATGCAGATTTAACTAACCAATTT
    GAATGGGATAATTTTGTAGAACGTTTTAAAACACTTATCGTTGAGCAAGAAATGGAAGAT
    GTGACATTGAACTTTGAATGTGGGCGCTTTATTGTGGCACATATTGGTTACTATGTGACA
    GAAGTGCTAGACATTAAGAAAGTACATGGTGCTTGGTATGCCATATTAAGAGGAGGTACG
    CAACAATTTAGACTGCCGGTATCTTGGCAACATAACCATCCTTTTGACATTTATCGCTAT
    AAGGACAATCCATATTCATTTGAAAAAGTTTCAATTTCGAGACAGGACACAACGTTAGTC
    GGTCAATTATGTACACCGAAAGATGTCTTTGCTAGAGAAGTACAGATAGACGCAATCAGT
    ACAGGCGACGTTATTGTTTTCAAATATGCAGGTGCATACGGATGGTCTATTTCACATCAC
    GATTTCTTAAGCCATCCACATCCTGAATTTATTTATTTAACACAAACAAAGGAGGATGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00082
  • symbol: SAOUHSC_00082
  • description: hypothetical protein
  • length: 400
  • theoretical pI: 6.29687
  • theoretical MW: 45759.5
  • GRAVY: -0.30025

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    pyridoxal-dependent decarboxylase, exosortase A system-associated (TIGR03099; HMM-score: 250.1)
    MetabolismAmino acid biosynthesisAspartate familydiaminopimelate decarboxylase (TIGR01048; EC 4.1.1.20; HMM-score: 203.6)
    MetabolismCentral intermediary metabolismPolyamine biosynthesisarginine decarboxylase (TIGR01273; EC 4.1.1.19; HMM-score: 38.7)
    MetabolismCentral intermediary metabolismPolyamine biosynthesiscarboxynorspermidine decarboxylase (TIGR01047; HMM-score: 31.4)
  • TheSEED:  
    Iron acquisition and metabolismSiderophoresSiderophore Staphylobactin Siderophore staphylobactin biosynthesis protein SbnH 
  • PFAM:
    TIM_barrel (CL0036) Orn_Arg_deC_N; Pyridoxal-dependent decarboxylase, pyridoxal binding domain (PF02784; HMM-score: 122.5)
    no clan definedOrn_DAP_Arg_deC; Pyridoxal-dependent decarboxylase, C-terminal sheet domain (PF00278; HMM-score: 50.3)
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 25.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 49
    • Cmax: 0.158
    • Cmax_pos: 49
    • Smax: 0.159
    • Smax_pos: 15
    • Smean: 0.101
    • D: 0.111
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQMYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRYAVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFGISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRFPLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIGYYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISRQDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIYLTQTKEDE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01592 (Fur*)
    Fur* (TF) important in Iron homeostasis

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Suzanne E Dale, Amanda Doherty-Kirby, Gilles Lajoie, David E Heinrichs
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
Infect. Immun.: 2004, 72(1);29-37
[PubMed:14688077] [WorldCat.org] (P p)