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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00103
  • pan locus tag?: SAUPAN000955000
  • symbol: SAOUHSC_00103
  • pan gene symbol?: phnE2
  • synonym:
  • product: phosphonates ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00103
  • symbol: SAOUHSC_00103
  • product: phosphonates ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 107319..108119
  • length: 801
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCCTTTAGAAATACCTACAAAGTATGACTCCCTTTTAAAGAAAAAGGTTTCTTTAAAA
    ACGAGTTTTACCTTCATGTTAATCATTGTGCTTATCATTTGGAGCTTCATTTATACGGGA
    TTTAATTTTGGAGATTTAATGATAGGAATACCTCAAATAGGTGATCTATTCAAACAAATG
    ATTCCACCTGATTTCGAGTATTTACAACAAATTACAACGCCAATGTTAGATACCATTCGA
    ATGGCTATCGTAAGTACAGTATTAGGTAGCATCGTTTCAATACCAATTGCGTTATTATGT
    GCTAGCAATATCGTTCATCAAAAGTGGATTTCAATACCCTCGCGCTTTATTTTAAATATA
    GTTCGTACTATTCCAGATTTGTTATTAGCAGCAATCTTTGTGGCTGTATTTGGAATCGGT
    CAAATTCCAGGGATATTAGCACTGTTTATTTTAACTATCTGTATTATTGGAAAATTATTA
    TATGAATCATTGGAAACGATAGATCCAGGTCCAATGGAAGCAATGACGGCTGTTGGCGCT
    AATAAAATAAAATGGATTGTTTTCGGTGTTGTACCACAAGCCATATCGTCATTTATGTCA
    TACGTATTATATGCATTTGAAGTAAATATACGTGCTTCAGCTGTGCTTGGATTAGTCGGC
    GCTGGCGGTATTGGATTGTTTTATGATCAAACACTTGGTTTATTTCAATATCCAAAAACA
    GCAACGATTATTTTATTTACTTTAGTTATCGTCGTCGTCATTGATTACATCAGTACGAAA
    GTGAGGGCACATCTCGCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    801

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00103
  • symbol: SAOUHSC_00103
  • description: phosphonates ABC transporter permease
  • length: 266
  • theoretical pI: 8.61947
  • theoretical MW: 29281.1
  • GRAVY: 0.957519

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 307.8)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 42.2)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 23.6)
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 18.8)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 18.8)
  • TheSEED:  
    ABC transporter alkylphosphonate (TC 3.A.1.9.1) ABC transporter, permease protein (cluster 12, methionine/phosphonates) 
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 54.9)
    Acyl_transf_3 (CL0316) DUF418; Protein of unknown function (DUF418) (PF04235; HMM-score: 14)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.16
    • Ymax_pos: 13
    • Cmax: 0.133
    • Cmax_pos: 43
    • Smax: 0.338
    • Smax_pos: 8
    • Smean: 0.264
    • D: 0.2
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFGDLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCASNIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLLYESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGLVGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]