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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00105
  • pan locus tag?: SAUPAN000957000
  • symbol: SAOUHSC_00105
  • pan gene symbol?: phnD
  • synonym:
  • product: phosphonate ABC transporter substrate-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00105
  • symbol: SAOUHSC_00105
  • product: phosphonate ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 109108..110064
  • length: 957
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATTTTAAGTGTTTATTTGTATTAATGTTAGCAGTCATTGTTTTTGCAGCAGCA
    TGTGGAAACTCAAGTTCTTTAGATAATCAAAAGAACGCTAGTAATGATTCGGATTCTAAA
    TCAGGAGGATACAAACCTAAAGAATTAACCGTTCAATTTGTACCTTCGCAAAATGCTGGA
    ACATTAGAAGCTAAAGCAAAACCATTAGAAAAATTACTATCTAAAGAATTAGGGATTCCA
    GTTAAAGTGTCTGTATCAACTAACTACAATACAATTGTTGAAGCTATGAAGTCTAAAAAA
    GTTGATGTTGGTTTCTTACCACCAACGGCATACACATTAGCACATGATCAAAAAGCAGCT
    GATTTATTATTACAAGCACAACGTTTCGGTGTAAAAGAAGATGGTTCAGCAAGTAAAGAA
    CTTGTAGATAGTTATAAATCAGAAATTCTTGTTAAAAAAGACTCAAAAATTAAAAGCTTG
    AAAGATTTAAAAGGTAAGAAAATTGCCTTACAAGATGTAACATCAACTGCTGGATATACA
    TTCCCACTTGCGATGTTAAAAAACGAAGCAGGTATTAATGCAACTAAAGATATGAAAATT
    GTGAATGTTAAAGGTCATGACCAAGCAGTTATCTCATTATTAAATGGAGATGTAGATGCT
    GCGGCTGTATTTAACGATGCACGTAATACTGTGAAAAAAGACCAACCAAATGTATTTAAA
    GACACACGAATTTTAAAATTAACACAAGCTATTCCGAATGACACAATTTCTGTAAGACCA
    GATATGGATAAAGATTTTCAAGAAAAATTGAAAAAAGCTTTTATAGACATTGCTAAATCA
    AAAGAAGGTCACAAAATTATTAGCGAAGTTTATTCACATGAAGGATACACAGAAACGAAA
    GATTCAAATTTCGACATTGTAAGAGAGTACGAAAAATTAGTTAAAGATATGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    957

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00105
  • symbol: SAOUHSC_00105
  • description: phosphonate ABC transporter substrate-binding protein
  • length: 318
  • theoretical pI: 9.66321
  • theoretical MW: 35000
  • GRAVY: -0.393082

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAnionsphosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein (TIGR01098; HMM-score: 257.2)
    phosphonate ABC transporter, periplasmic phosphonate binding protein (TIGR03431; HMM-score: 173.6)
    putative selenate ABC transporter periplasmic binding protein (TIGR04553; HMM-score: 76.2)
    MetabolismTransport and binding proteinsOtherABC transporter, substrate-binding protein, aliphatic sulfonates family (TIGR01728; HMM-score: 54.3)
    taurine ABC transporter, periplasmic binding protein (TIGR01729; HMM-score: 46.2)
    MetabolismTransport and binding proteinsUnknown substrateTRAP transporter solute receptor, TAXI family (TIGR02122; HMM-score: 37.2)
    MetabolismTransport and binding proteinsAmino acids, peptides and amineslysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 22.2)
  • TheSEED:  
    ABC transporter alkylphosphonate (TC 3.A.1.9.1) ABC transporter, substrate-binding protein (cluster 12, methionine/phosphonates) 
  • PFAM:
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 241.7)
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 48.4)
    SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 44.1)
    NMT1_2; NMT1-like family (PF13379; HMM-score: 24.1)
    Mycoplasma_p37; High affinity transport system protein p37 (PF06646; HMM-score: 23.8)
    NMT1_3; NMT1-like family (PF16868; HMM-score: 21.3)
    Hexosaminidase (CL0546) Glyco_hydro_20b; Glycosyl hydrolase family 20, domain 2 (PF02838; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: VFAAACG
  • SignalP: Signal peptide length 34 aa
    • Ymax: 0.398
    • Ymax_pos: 34
    • Cmax: 0.227
    • Cmax_pos: 34
    • Smax: 0.985
    • Smax_pos: 17
    • Smean: 0.856
    • D: 0.576
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSLKDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]