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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00201
  • pan locus tag?: SAUPAN001102000
  • symbol: SAOUHSC_00201
  • pan gene symbol?: opp-5A
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00201
  • symbol: SAOUHSC_00201
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 224235..225647
  • length: 1413
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCATTCATCTGGCAAAGACTTAAATATTTCGTTACCGTTAAAAACAAAGTCTATTGCA
    CCTTATGAAACAGATGTACCAGTTAAGATTGGTGCTGCAGAATCATTATTTAAAACAAAT
    GATCAAGGAAAAATAGAAAAAGCATTAGTGAAATCGTATCATCAACCAAATGATACAACG
    TTAGATATAGAATTAAAAGATAATATTAAATTTCAAAACGGTCAAAAATTGACTGCAGAA
    AAAGTGAAATCTAGCCTTGAAAATAGCATGAAAAAAAGCGACTTGGTCAAATATTCATTA
    CCAATATCATCAATTACCGCTAAAGGTCAAAAACTGACAATTAAAACCAACTCCGCTTAC
    CCTGAACTTGTATCTGAATTAGCTAATCCTTTTATGGCAATTTATGATACAGATGCTAAA
    TCAGATGTTAATCAAACTCCTGTTGGTACAGGCCCTTACCAAATAAAAGATTATAAGCAA
    TCTCGAAAAATATCATTGTCGAATTTTAAGGACTATTGGCAAGGTAAACCGAAACTTGAT
    CATATTACTGTGACGTACCAAGAAGACGGCAATAATCGCGTCAGAAATTTAGAATCTCAA
    AAAGATGATTTAATAACTGATGTCCCAGTTAATAAAGTTCAAGACATAGAAAATAATCAA
    AATTTAAAAGTGTCAAAAGAATCTGGATTTAGAACTTCTTTACTTATGTATAATCATACT
    AATAAAAAAATGACTAAATCCGTTCGTGAAGCATTAGATCATATCATTGATAGACAAGGT
    ATTGCAGATCATATTTATCAAGGTTATGCGAAACCTGCAACGAGTCCATTTAATGATAAA
    ATTCCATATATTAAAGAACCTAAGTTAACCAAACAAAATATCGAACAAGCAAAAATGTTA
    TTAGCTAAAGATGGTTATACAAAAGAACACCCTTTAAAAATTAAGTTAATTACGTATGAC
    GGTCGTCCAGAGCTATCGAAAATTGCGCAAGTATTACAATCAGATGCTAAAAAAGCAAAT
    ATTGAAATCGACATTAAAAGTGTTGATGACATAGAAGGTTACTTAAAAGACCGTTCTGCA
    TGGGATGCAACGATGTATAGTTTCGGAACAATTCCTCGTGGAGATACAGGTTATTTCTTT
    AATCAAGCATATAAAAAAGATGGTGCTATTAATAAAGGAGACTACAATAATAGCAACGTC
    GATGATTTAATTAATCAATTGAATCATACTGTAGATGTTAAGGAACGTCACAATATTTCT
    AATGATATTATTAAATTATCAAGTCGAGATGTACCAAACAGTTATATTGCTTACAACGAT
    CAAATAGTCGCTGCTAACTCTAAGGTTAAAAATTATAAAGTAACACCTGAAGGTATCTAT
    TTAATCGATTATCGAACAACGATTGAACGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1413

Protein[edit source | edit]

Protein Data Bank: 3RQT

General[edit source | edit]

  • locus tag: SAOUHSC_00201
  • symbol: SAOUHSC_00201
  • description: hypothetical protein
  • length: 470
  • theoretical pI: 9.38724
  • theoretical MW: 53219.7
  • GRAVY: -0.74617

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 117.8)
    MetabolismTransport and binding proteinsUnknown substrateABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 42.4)
  • TheSEED:  
    Membrane TransportABC transportersABC transporter oligopeptide (TC 3.A.1.5.1) Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) 
    Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) 
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 223.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.2
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.161
    • Ymax_pos: 33
    • Cmax: 0.156
    • Cmax_pos: 33
    • Smax: 0.304
    • Smax_pos: 18
    • Smean: 0.185
    • D: 0.171
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J. Bacteriol.: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)
Aurelia Hiron, Brunella Posteraro, Marie Carrière, Laetitia Remy, Cécile Delporte, Marilena La Sorda, Maurizio Sanguinetti, Vincent Juillard, Elise Borezée-Durant
A nickel ABC-transporter of Staphylococcus aureus is involved in urinary tract infection.
Mol. Microbiol.: 2010, 77(5);1246-60
[PubMed:20662775] [WorldCat.org] [DOI] (I p)