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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00242
  • pan locus tag?: SAUPAN001157000
  • symbol: SAOUHSC_00242
  • pan gene symbol?: rbsR
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00242
  • symbol: SAOUHSC_00242
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 262382..263380
  • length: 999
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAAAGTGTCAATTAAAGATGTTGCTAGAGAAGCTGGTGTATCAGTTACAACTGTG
    TCACATATTTTAAATCATAATGATAGTCGTTTTTCCGCAACAACGATAAAAAACGTACAT
    GCTGTTTCAGAACGTTTAGGCTATGCCCCTAATAAACATGCAAAACAATTGCGCGGCAGT
    AAAATTCAAACTATTGGCGTCATTTTGCCTAGCTTAACAAATCCGTTTTTCTCAGCACTG
    ATGCAAAGTATTCATGACCATAAACCATCTGATGTTGATTTATGCTTTTTAACATCTACA
    GCAACTGATTTGTATGACAATATTAAACATTTAATTGATCGAGGTATTGACGGATTAATT
    ATCGCACAATACATATCATCCCCGGACGCCCTAAATAACTATCTAAAGAAACATCATGTA
    CCTTATGTCGTACTGGATCAAAATGACCATCAAGGCTATACAGATTTTGTTCGGACAAAT
    GAATATCAAGGTGGACAACTTGCAGCACAACATTTAATAGAACTCGGTCACAACCATATG
    ATAATTGTTGCACCATATGACATGATGGCGAATATGTCGACTCGTGTCGCTGGATTTGTC
    GATACTTTGCGCGCGAATCAATTGCCAGAACCACAAATCGTCCATACTGAATTATCTAAG
    CGCGGTGGGCTAACCATTGTTGATGACATCATGGTTCAATCTGCCACTGCAATCTTCGCT
    ATTAACGATGAACTCGCTATTGGCATTTTACGAGGACTAATTGAACATGGCATCAGTATC
    CCGAAAGATATCTCATTAATAGGTTATGACGACATTGATTATGCAGCGTACGTCTCGCCA
    CCTTTAACTACTGTGGCACAACCTATAACTGATATTGGCAAAACATCTTTAACCTTATTA
    CTTCAACGATTACAGCACTTAGATAAATCCATTGATATGATTGAATTACCAACGACTTTA
    AAAATTCGTGCAACAACTGGCTATCATCTTTCAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00242
  • symbol: SAOUHSC_00242
  • description: hypothetical protein
  • length: 332
  • theoretical pI: 6.76486
  • theoretical MW: 36657.7
  • GRAVY: -0.0650602

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionscatabolite control protein A (TIGR01481; HMM-score: 172.7)
    Signal transductionRegulatory functionsDNA interactionsD-fructose-responsive transcription factor (TIGR02417; HMM-score: 117.9)
    Signal transductionRegulatory functionsDNA interactionstrehalose operon repressor (TIGR02405; HMM-score: 74.3)
    Signal transductionRegulatory functionsDNA interactionstranscriptional repressor BetI (TIGR03384; HMM-score: 13.9)
    Genetic information processingProtein synthesistRNA aminoacylationaspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (TIGR00132; EC 6.3.5.-; HMM-score: 11)
  • TheSEED:  
    CarbohydratesMonosaccharidesD-ribose utilization Ribose operon repressor 
  • PFAM:
    Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 122.2)
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 82.2)
    Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 61.8)
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 57.3)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20)
    HTH_3; Helix-turn-helix (PF01381; HMM-score: 18.8)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 15.3)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 14.6)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 14)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 13.5)
    HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 13.4)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.4)
    CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 13.1)
    IF2_N; Translation initiation factor IF-2, N-terminal region (PF04760; HMM-score: 13)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 12.7)
    no clan definedPucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 12.6)
    TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 10.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.224
    • Ymax_pos: 33
    • Cmax: 0.222
    • Cmax_pos: 33
    • Smax: 0.439
    • Smax_pos: 3
    • Smean: 0.274
    • D: 0.243
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTNEYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 (SigB*) [2] [3] other strains
    SigB* (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  3. 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]