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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00389
  • pan locus tag?: SAUPAN002107000
  • symbol: SAOUHSC_00389
  • pan gene symbol?: ssl4
  • synonym:
  • product: superantigen-like protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00389
  • symbol: SAOUHSC_00389
  • product: superantigen-like protein
  • replicon: chromosome
  • strand: +
  • coordinates: 391681..392607
  • length: 927
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAATAACAACGATTGCTAAAACAAGTTTAGCACTAGGCCTTTTAACAACAGGTGTA
    ATCACAACGACAACGCAAGCAGCAAACGCGACAACACTATCTTCCACTAAAGTGGAAGCA
    CCACAATCAACACCGCCCTCAACTAAAATAGAAGCACCGCAATCAAAACCAAACGCGACA
    ACACCGCCCTCAACTAAAGTAGAAGCACCGCAACAAACAGCAAATGCGACAACACCGCCT
    TCAACTAAAGTGACAACACCTCCATCAACAAACACGCCACAACCAATGCAATCTACTAAA
    TCAGACACACCACAATCGCCAACCACAAAACAAGTACCAACAGAAATAAATCCTAAATTT
    AAAGATTTAAGAGCGTATTATACGAAACCAAGTTTAGAATTTAAAAATGAGATTGGTATT
    ATTTTAAAAAAATGGACGACAATAAGATTTATGAATGTTGTCCCAGATTATTTCATATAT
    AAAATTGCTTTAGTTGGTAAAGATGATAAAAAATATGGTGAAGGAGTACATAGGAATGTC
    GATGTATTTGTCGTTTTAGAAGAAAATAATTACAATCTGGAAAAATATTCTGTCGGTGGT
    ATCACAAAGAGTAATAGTAAAAAAGTTGATCACAAAGCAGGAGTAAGAATTACTAAGGAA
    GATAATAAAGGTACAATCTCTCATGATGTTTCAGAATTCAAGATTACTAAAGAACAGATT
    TCCTTGAAAGAACTTGATTTTAAATTGAGAAAACAACTTATTGAAAAAAATAATCTGTAC
    GGTAACGTTGGTTCAGGTAAAATTGTTATTAAAATGAAAAACGGTGGAAAGTACACGTTT
    GAATTGCACAAAAAATTACAAGAAAATCGCATGGCAGATGTCATAGATGGCACTAATATT
    GATAACATTGAAGTGAATATAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

Protein[edit source | edit]

Protein Data Bank: 3URY
Protein Data Bank: 4RCO

General[edit source | edit]

  • locus tag: SAOUHSC_00389
  • symbol: SAOUHSC_00389
  • description: superantigen-like protein
  • length: 308
  • theoretical pI: 10.2076
  • theoretical MW: 34071.7
  • GRAVY: -0.620779

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Exotoxin 8 
  • PFAM:
    OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 113.4)
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 31.9)
    no clan definedHamartin; Hamartin protein (PF04388; HMM-score: 12.6)
    Toxin_10; Insecticidal Crystal Toxin, P42 (PF05431; HMM-score: 12.5)
    TPR (CL0020) Suf; Suppressor of forked protein (Suf) (PF05843; HMM-score: 12)
    no clan definedTHEG4; Testis highly expressed protein 4 (PF15834; HMM-score: 7.9)
    SOG2; RAM signalling pathway protein (PF10428; HMM-score: 7.3)
    Ndc1_Nup; Nucleoporin protein Ndc1-Nup (PF09531; HMM-score: 5.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: TQAANATT
  • SignalP: Signal peptide length 31 aa
    • Ymax: 0.749
    • Ymax_pos: 31
    • Cmax: 0.659
    • Cmax_pos: 31
    • Smax: 0.95
    • Smax_pos: 14
    • Smean: 0.892
    • D: 0.805
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKITTIAKTSLALGLLTTGVITTTTQAANATTLSSTKVEAPQSTPPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSDTPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIYKIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRITKEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGGKYTFELHKKLQENRMADVIDGTNIDNIEVNIK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol. Med. Microbiol.: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)