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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00397
  • pan locus tag?: SAUPAN002121000
  • symbol: SAOUHSC_00397
  • pan gene symbol?: hsdM1
  • synonym:
  • product: type I restriction-modification system subunit M

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00397
  • symbol: SAOUHSC_00397
  • product: type I restriction-modification system subunit M
  • replicon: chromosome
  • strand: +
  • coordinates: 399449..400921
  • length: 1473
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGCGAGTGAATTCCGTAATTACATTTTAGGCTTGATTTTCTATCGCTTCTTATCT
    GAAAAAGCGGAACAAGAATATGCAGATGCCTTGTCAGGTGAAGACATCACGTATCAAGAA
    GCATGGGCAGACGAAGAATACCGTGAAGACTTAAAAGCAGAATTAATTGACCAAGTCGGT
    TACTTCATTGAGCCAGAAGATTTATTCAGTGCGATGATTCGTGAAATTGAAACGCAAGAT
    TTCGATATCGAACACCTGGCGACGGCAATTCGTAAAGTTGAAACATCAACATTAGGTGAA
    GAAAGTGAAAATGACTTTATCGGTCTGTTCAGCGATATGGATTTGAGTTCAACGCGACTA
    GGTAACAATGTCAAAGAACGTACTGCTTTAATCTCTAAAGTCATGGTTAATCTTGACGAC
    TTACCATTCGTTCACAGTGACATGGAAATTGATATGTTAGGTGATGCATATGAATTCCTA
    ATTGGGCGCTTTGCGGCGACAGCGGGTAAAAAAGCAGGCGAGTTCTATACACCACAACAA
    GTATCTAAGATACTGGCGAAGATTGTCACAGACGGTAAAGATAAATTACGTCACGTGTAT
    GACCCAACATGTGGTTCAGGTTCACTGTTGTTACGTGTTGGTAAAGAAACACAAGTGTAT
    CGTTATTTCGGTCAAGAACGTAACAATACTACATACAACTTAGCACGCATGAATATGTTA
    TTACATGATGTGCGTTATGAGAACTTCGATATCCGTAATGATGACACATTGGAAAACCCA
    GCCTTTTTAGGCAATACATTTGATGCGGTTATTGCGAACCCACCGTATAGTGCGAAATGG
    ACTGCAGATTCAAAGTTTGAAAATGACGAACGATTCAGTGGTTACGGCAAACTTGCGCCT
    AAGTCTAAAGCAGACTTTGCCTTTATTCAACACATGGTACATTACCTAGACGATGAAGGT
    ACCATGGCCGTTGTACTCCCACATGGTGTATTATTCCGAGGTGCTGCAGAAGGTGTCATT
    CGTCGTTATTTAATTGAAGAAAAGAACTACTTAGAAGCTGTGATTGGTTTGCCAGCGAAT
    ATTTTCTATGGGACAAGTATTCCAACATGTATTTTAGTATTTAAAAAATGTCGCCAACAA
    GACGACAACGTACTATTTATCGATGCATCCAATGATTTTGAAAAAGGAAAAAATCAAAAT
    CATTTAAGCGATGCCCAAGTCGAACGTATTATAGACACATATAAGCGTAAGGAAACAATT
    GATAAATATAGCTACAGCGCGACACTACAAGAGATTGCCGATAACGATTACAACCTAAAT
    ATACCGAGATATGTCGATACATTCGAAGAAGAAGCACCGATTGATTTAGATCAAGTCCAA
    CAAGATTTGAAAAATATCGATAAAGAAATCGCAGAAATTGAGCAAGAAATCAATGCATAC
    CTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1473

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00397
  • symbol: SAOUHSC_00397
  • description: type I restriction-modification system subunit M
  • length: 490
  • theoretical pI: 4.28129
  • theoretical MW: 56138.4
  • GRAVY: -0.452041

Function[edit source | edit]

  • reaction:
    EC 2.1.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismRestriction/modificationtype I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 434.9)
    Genetic information processingDNA metabolismRestriction/modificationtype II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 37.5)
    Genetic information processingProtein fateProtein modification and repairprotein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 36.5)
    Genetic information processingProtein fateProtein modification and repairmethyltransferase, HemK family (TIGR00536; HMM-score: 15.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.5)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationprotein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 14.2)
  • TheSEED:  
    DNA MetabolismDNA Metabolism - no subcategoryRestriction-Modification System Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72) 
    DNA Metabolism - no subcategoryType I Restriction-Modification Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72) 
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 372.5)
    no clan definedHsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 50.1)
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 24.6)
    Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 23.7)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16.2)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.8)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 14.5)
    N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.162
    • Ymax_pos: 31
    • Cmax: 0.23
    • Cmax_pos: 44
    • Smax: 0.267
    • Smax_pos: 22
    • Smean: 0.087
    • D: 0.133
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]