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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00422
  • pan locus tag?: SAUPAN002175000
  • symbol: SAOUHSC_00422
  • pan gene symbol?: mccB
  • synonym:
  • product: trans-sulfuration enzyme family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00422
  • symbol: SAOUHSC_00422
  • product: trans-sulfuration enzyme family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 423274..424416
  • length: 1143
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAACAAGAAAACTAAATTAATTCATGGTGGGCACACAACAGACGATTATACAGGTGCC
    GTTACAACACCAATTTATCAAACAAGTACATATTTACAAGATGATATTGGTGATTTACGT
    CAAGGATATGAATATTCTCGTACTGCGAATCCAACAAGAAGTTCTGTAGAAAGCGTTATT
    GCGACATTAGAAAATGGCAAACATGGCTTTGCATTTAGTTCAGGTGTTGCAGCAATCAGT
    GCAGTTGTTATGCTGTTGGACAAAGGAGATCATATTATTTTAAATTCAGATGTATACGGC
    GGTACTTATCGCGCATTGACAAAAGTATTTACACGATTTGGCATTGAAGTGGATTTTGTA
    GATACAACGCATACAGATTCAATTGTACAAGCGATACGCCCAACAACAAAGATGTTGTTT
    ATTGAAACACCTTCTAATCCATTATTACGTGTTACTGACATTAAAAAGTCTGCTGAAATT
    GCGAAAGAACACGGTTTGATTTCAGTTGTTGATAACACATTTATGACACCTTATTATCAG
    AATCCATTAGATTTAGGTATCGATATTGTCTTACATTCTGCAACGAAATATTTAGGTGGA
    CATAGTGATGTCGTTGCTGGTTTAGTTGCAACATCGGATGACAAGCTTGCAGAACGTTTA
    GCATTTATTTCAAATTCAACAGGTGGCATTTTAGGACCTCAAGATAGCTATTTACTTGTG
    AGGGGTATTAAAACATTAGGTTTACGTATGGAACAAATTAATCGCAGCGTTATTGAAATT
    ATTAAAATGTTACAAGCACATCCAGCTGTGCAACAAGTGTTCCATCCAAGTATTGAAAGT
    CATTTAAATCATGATGTCCATATGGCTCAAGCGGATGGCCATACAGGTGTGATTGCATTT
    GAAGTGAAAAATACAGAAAGTGCCAAACAATTGATTAAAGCAACATCGTATTACACATTA
    GCTGAAAGTTTAGGTGCAGTGGAAAGTTTAATTTCAGTACCTGCATTGATGACACATGCA
    TCCATTCCAGCAGATATTCGAGCTAAAGAAGGTATTACAGACGGACTTGTAAGAATTTCT
    GTAGGTATTGAAGATACTGAAGATTTAGTCGATGATTTAAAACAAGCACTAGATACTTTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00422
  • symbol: SAOUHSC_00422
  • description: trans-sulfuration enzyme family protein
  • length: 380
  • theoretical pI: 5.67217
  • theoretical MW: 41278.5
  • GRAVY: -0.0436842

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 398.3)
    MetabolismAmino acid biosynthesisAspartate familyO-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 392.3)
    MetabolismEnergy metabolismAmino acids and aminesmethionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 379.4)
    MetabolismAmino acid biosynthesisAspartate familyO-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 313.9)
    MetabolismAmino acid biosynthesisSerine familyO-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 313.9)
    MetabolismAmino acid biosynthesisAspartate familyO-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 312.7)
    MetabolismAmino acid biosynthesisAspartate familycystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 238.7)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 31.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminthreonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 22.7)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01976; HMM-score: 21.6)
    MetabolismAmino acid biosynthesisHistidine familyhistidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 21.2)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 17.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, SufS family (TIGR01979; HMM-score: 16.7)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 16.7)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 13.8)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 13.3)
    enduracididine biosynthesis enzyme MppP (TIGR04462; EC 2.-.-.-; HMM-score: 13.3)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 13)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 11)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization Cystathionine gamma-lyase (EC 4.4.1.1) 
    Lysine, threonine, methionine, and cysteineCysteine Biosynthesis Cystathionine gamma-lyase (EC 4.4.1.1) 
    Lysine, threonine, methionine, and cysteineMethionine Biosynthesis Cystathionine gamma-lyase (EC 4.4.1.1) 
    Lysine, threonine, methionine, and cysteineMethionine Degradation Cystathionine gamma-lyase (EC 4.4.1.1) 
  • PFAM:
    PLP_aminotran (CL0061) Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 487.7)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 34.3)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 28)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 20.1)
    no clan definedIF-2; Translation-initiation factor 2 (PF11987; HMM-score: 15.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.12
    • Ymax_pos: 11
    • Cmax: 0.111
    • Cmax_pos: 67
    • Smax: 0.165
    • Smax_pos: 1
    • Smean: 0.139
    • D: 0.127
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEIAKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01732 (CymR*)
    CymR* (TF) important in Cysteine metabolismRegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]