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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00424
  • pan locus tag?: SAUPAN002177000
  • symbol: SAOUHSC_00424
  • pan gene symbol?: metP2
  • synonym:
  • product: ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00424
  • symbol: SAOUHSC_00424
  • product: ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 425739..426398
  • length: 660
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTGGTTCTGATTTAGACAGTGCACAGTTATTACAAGCATTGTACGAAACGCTATAT
    ATGGTATCTATTGCTTTATTTTTAGGAGCAGTGATTGGTATTCCATTAGGTGTCTTATTG
    GTAATTACTCGAAAACAAGGCATATGGCCCAATATAGTGATACATCAAGTTTTAAATCCT
    TTAATCAATATTTTAAGGTCACTACCATTTATTATTTTGTTAATTGCGATTGTGCCATTC
    ACAAAATTAGTAGTAGGTACTTCAATTGGTACGACTGCTGCCATCGTGCCTTTAACAGTA
    TATGTGGCACCTTACATTGCAAGACTTGTTGAAAACTCATTATTGGAAGTAGACGAGGGG
    ATTATTGAAGCGGCGAAAGCGATGGGCGCTTCACCACTACAAATCATTAGATATTTTTTA
    ATTCCTGAAGCTTTAGGTTCGTTAGTATTAGCAATTACCACTGCGATTATTGGACTTATT
    GGAAGTACGGCGATGGCAGGAGCTGTTGGCGGTGGTGGTATAGGAGACTTAGCTTTAGTG
    TATGGTTATCAAAGATTTGATACGACGGTCATTATTATTACCGTTATTGTATTAGTCATT
    ATTGTCCAAGTGATTCAAACGCTAGGGAATGTTCTAGCTAGATTCATACGTAGACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00424
  • symbol: SAOUHSC_00424
  • description: ABC transporter permease
  • length: 219
  • theoretical pI: 7.66251
  • theoretical MW: 23309.9
  • GRAVY: 1.15479

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 73.4)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 38.3)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 33.2)
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 31.8)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 22.7)
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 21.9)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 20.3)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 19)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 18.4)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.2)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 16.6)
    MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 14.8)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis Methionine ABC transporter permease protein 
    Lysine, threonine, methionine, and cysteineMethionine Degradation Methionine ABC transporter permease protein 
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 71.6)
    FtsX; FtsX-like permease family (PF02687; HMM-score: 7.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 48
    • Cmax: 0.153
    • Cmax_pos: 48
    • Smax: 0.121
    • Smax_pos: 44
    • Smean: 0.052
    • D: 0.09
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITRKQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYVAPYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLIGSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRRH

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01732 (CymR*)
    CymR* (TF) important in Cysteine metabolism

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]