NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0436 [new locus tag: SAUSA300_RS02335 ]
• pan locus tag^?: SAUPAN002177000
• symbol: SAUSA300_0436
• pan gene symbol^?: metP2
• synonym:
• product: ABC transporter permease

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0436 [new locus tag: SAUSA300_RS02335 ]
• symbol: SAUSA300_0436
• product: ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 489809..490468
• length: 660
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914676 NCBI
• RefSeq: YP_493149 NCBI
• MicrobesOnline: 1291951 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGTTTGGTTCTGATTTAGACAGTGCACAGTTATTACAAGCATTGTACGAAACGCTATAT
  ATGGTATCTATTGCTTTATTTTTAGGAGCAGTGATTGGTATTCCATTAGGTGTCTTATTG
  GTAATTACTCGAAAACAAGGCATATGGCCCAATATAGTGATACATCAAGTTTTAAATCCT
  TTAATCAATATTTTAAGGTCACTACCATTTATTATTTTGTTAATTGCGATTGTGCCATTC
  ACAAAATTAGTAGTAGGTACTTCAATTGGTACGACTGCTGCCATCGTGCCTTTAACAGTA
  TATGTGGCACCTTACATTGCAAGACTTGTTGAAAACTCATTATTGGAAGTAGACGAGGGG
  ATTATTGAAGCGGCGAAAGCGATGGGCGCTTCACCACTACAAATCATTAGATATTTTTTA
  ATTCCTGAAGCTTTAGGTTCGTTAGTATTAGCAATTACCACTGCGATTATTGGACTTATT
  GGAAGTACGGCGATGGCAGGAGCTGTTGGCGGTGGTGGTATAGGAGACTTAGCTTTAGTG
  TATGGTTATCAAAGATTTGATACGACGGTCATTATTATTACCGTTATTGTATTAGTCATT
  ATTGTCCAAGTGATTCAAACGCTAGGGAATGTTCTAGCTAGATTCATACGTAGACATTAA
  60
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⊟Protein

⊟General

• locus tag: SAUSA300_0436 [new locus tag: SAUSA300_RS02335 ]
• symbol: SAUSA300_0436
• description: ABC transporter permease
• length: 219
• theoretical pI: 7.66251
• theoretical MW: 23309.9
• GRAVY: 1.15479

⊟Function

• reaction:
• TIGRFAM:
  MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 73.4)
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 38.3)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 33.2)
  MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 31.8)
  MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 22.7)
  MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 21.9)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 20.3)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 19)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 18.4)
  MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.2)
  MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 16.6)
  MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 14.8)
• TheSEED:  
  Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis Methionine ABC transporter permease protein 
  Lysine, threonine, methionine, and cysteineMethionine Degradation Methionine ABC transporter permease protein 
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 71.6)
  FtsX; FtsX-like permease family (PF02687; HMM-score: 7.4)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.114
  • Ymax_pos: 48
  • Cmax: 0.153
  • Cmax_pos: 48
  • Smax: 0.121
  • Smax_pos: 44
  • Smean: 0.052
  • D: 0.09
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 87161549 NCBI
• RefSeq: YP_493149 NCBI
• UniProt: A0A0H2XHC9 UniProt

⊟Protein sequence

• MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITRKQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYVAPYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLIGSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRRH

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0435 > SAUSA300_0436 > SAUSA300_0437

⊟Regulation

• sigma factor:
• regulator: SAUSA300_1583 (CymR*)

  CymR*

  (TF)

  important in Cysteine metabolism

  RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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