NCBI: 10-JUN-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0436 [new locus tag: SAUSA300_RS02335 ]
- pan locus tag?: SAUPAN002177000
- symbol: SAUSA300_0436
- pan gene symbol?: metP2
- synonym:
- product: ABC transporter permease
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SAUSA300_0436 [new locus tag: SAUSA300_RS02335 ]
- symbol: SAUSA300_0436
- product: ABC transporter permease
- replicon: chromosome
- strand: +
- coordinates: 489809..490468
- length: 660
- essential: unknown other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 3914676 NCBI
- RefSeq: YP_493149 NCBI
- MicrobesOnline: 1291951 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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601ATGTTTGGTTCTGATTTAGACAGTGCACAGTTATTACAAGCATTGTACGAAACGCTATAT
ATGGTATCTATTGCTTTATTTTTAGGAGCAGTGATTGGTATTCCATTAGGTGTCTTATTG
GTAATTACTCGAAAACAAGGCATATGGCCCAATATAGTGATACATCAAGTTTTAAATCCT
TTAATCAATATTTTAAGGTCACTACCATTTATTATTTTGTTAATTGCGATTGTGCCATTC
ACAAAATTAGTAGTAGGTACTTCAATTGGTACGACTGCTGCCATCGTGCCTTTAACAGTA
TATGTGGCACCTTACATTGCAAGACTTGTTGAAAACTCATTATTGGAAGTAGACGAGGGG
ATTATTGAAGCGGCGAAAGCGATGGGCGCTTCACCACTACAAATCATTAGATATTTTTTA
ATTCCTGAAGCTTTAGGTTCGTTAGTATTAGCAATTACCACTGCGATTATTGGACTTATT
GGAAGTACGGCGATGGCAGGAGCTGTTGGCGGTGGTGGTATAGGAGACTTAGCTTTAGTG
TATGGTTATCAAAGATTTGATACGACGGTCATTATTATTACCGTTATTGTATTAGTCATT
ATTGTCCAAGTGATTCAAACGCTAGGGAATGTTCTAGCTAGATTCATACGTAGACATTAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SAUSA300_0436 [new locus tag: SAUSA300_RS02335 ]
- symbol: SAUSA300_0436
- description: ABC transporter permease
- length: 219
- theoretical pI: 7.66251
- theoretical MW: 23309.9
- GRAVY: 1.15479
⊟Function[edit source | edit]
- reaction:
- TIGRFAM: Transport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 73.4)choline ABC transporter, permease protein (TIGR03416; HMM-score: 38.3)ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 33.2)Transport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 31.8)Transport and binding proteinsAmino acids, peptides and aminesamino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 22.7)Transport and binding proteinsAnionsphosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 21.9)ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 20.3)NifC-like ABC-type porter (TIGR01581; HMM-score: 19)2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 18.4)Transport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.2)Transport and binding proteinsAnionssulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 16.6)Transport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 14.8)
- TheSEED:
- PFAM: BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 71.6)FtsX; FtsX-like permease family (PF02687; HMM-score: 7.4)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 5
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular Possibility: 0.17
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.114
- Ymax_pos: 48
- Cmax: 0.153
- Cmax_pos: 48
- Smax: 0.121
- Smax_pos: 44
- Smean: 0.052
- D: 0.09
- predicted transmembrane helices (TMHMM): 5
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITRKQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYVAPYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLIGSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRRH
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
⊟Regulation[edit source | edit]
- sigma factor:
- regulator: SAUSA300_1583 (CymR*)
CymR* (TF) important in Cysteine metabolism RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]