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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00555
  • pan locus tag?: SAUPAN002349000
  • symbol: SAOUHSC_00555
  • pan gene symbol?:
  • synonym:
  • product: haloacid dehalogenase-like hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00555
  • symbol: SAOUHSC_00555
  • product: haloacid dehalogenase-like hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 562979..563626
  • length: 648
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    TTGAAGTTTGACAATTATATTTTTGATTTTGATGGTACGTTGGCAGACACGAAAAAATGT
    GGTGAAGTAGCAACACAAAGTGCATTTAAAGCATGTGGCTTAACGGAACCATCATCTAAA
    GAAATAACGCATTATATGGGAATACCTATTGAAGAATCATTTTTAAAATTAGCAGACCGA
    CCATTAGATGAAGCAGCATTAGCAAAGTTAATCGATACATTTAGACATACATATCAATCT
    ATTGAAAAGGACTATATTTATGAATTTGCGGGTATAACTGAAGCCATTACAAGTTTGTAT
    AACCAAGGGAAAAAACTTTTCGTGGTGTCTAGTAAGAAGAGTGATGTATTAGAAAGAAAT
    TTATCGGCTATTGGATTAAATCACTTGATTACCGAAGCTGTTGGATCCGATCAAGTAAGT
    GCATATAAACCAAATCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGC
    CAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGT
    ATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAAT
    CCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    648

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00555
  • symbol: SAOUHSC_00555
  • description: haloacid dehalogenase-like hydrolase
  • length: 215
  • theoretical pI: 4.79367
  • theoretical MW: 23866.6
  • GRAVY: -0.26186

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismEnergy metabolismSugarsphosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 94)
    AHBA synthesis associated protein (TIGR01454; HMM-score: 77.9)
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 69.3)
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 66.4)
    beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 57)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIIA (TIGR01662; HMM-score: 52.6)
    HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 48.3)
    haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 46.4)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 44.6)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesbeta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 41.5)
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 38.6)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 34.1)
    HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 33.7)
    MetabolismCentral intermediary metabolismOtherphosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 32)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 29.9)
    MetabolismAmino acid biosynthesisSerine familyphosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 29.2)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharides3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 27.1)
    Unknown functionEnzymes of unknown specificityCof-like hydrolase (TIGR00099; HMM-score: 26.1)
    HAD phosphatase, family IIIC (TIGR01681; HMM-score: 25.3)
    Unknown functionEnzymes of unknown specificityHAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 24.2)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIB (TIGR01484; HMM-score: 24.1)
    phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 23.4)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 23.2)
    pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 22.7)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 19.8)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIA (TIGR01460; HMM-score: 19.2)
    FkbH domain (TIGR01686; HMM-score: 18.7)
    DNA 3'-phosphatase (TIGR01664; EC 3.1.3.32; HMM-score: 18.2)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IB (TIGR01490; HMM-score: 17.3)
    MetabolismCentral intermediary metabolismOther2,3-diketo-5-methylthio-1-phosphopentane phosphatase (TIGR01489; HMM-score: 16.9)
    HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 16)
    epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 14.6)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 14.2)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 13.8)
    HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 13)
    MetabolismAmino acid biosynthesisHistidine familyhistidinol-phosphatase (TIGR01261; EC 3.1.3.15; HMM-score: 12.5)
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 12.3)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismGlycolate, glyoxylate interconversions Phosphoglycolate phosphatase (EC 3.1.3.18) 
    DNA MetabolismDNA repair2-phosphoglycolate salvage Phosphoglycolate phosphatase (EC 3.1.3.18) 
  • PFAM:
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 153.8)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 51.3)
    Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 49.6)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 37.9)
    Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 29.5)
    PNK3P; Polynucleotide kinase 3 phosphatase (PF08645; HMM-score: 15.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.12
    • Ymax_pos: 32
    • Cmax: 0.116
    • Cmax_pos: 41
    • Smax: 0.154
    • Smax_pos: 23
    • Smean: 0.096
    • D: 0.111
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITHYMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQGKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNSQQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]