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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00620
  • pan locus tag?: SAUPAN002485000
  • symbol: SAOUHSC_00620
  • pan gene symbol?: sarA
  • synonym:
  • product: accessory regulator A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00620
  • symbol: SAOUHSC_00620
  • product: accessory regulator A
  • replicon: chromosome
  • strand: -
  • coordinates: 611301..611675
  • length: 375
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    ATGGCAATTACAAAAATCAATGATTGCTTTGAGTTGTTATCAATGGTCACTTATGCTGAC
    AAATTAAAAAGTTTAATTAAAAAGGAATTTTCAATTAGCTTTGAAGAATTCGCTGTATTG
    ACATACATCAGCGAAAACAAAGAGAAAGAATACTATCTTAAAGATATTATTAATCATTTA
    AACTACAAACAACCACAAGTTGTTAAAGCAGTTAAAATTTTATCTCAAGAAGATTACTTC
    GATAAAAAACGTAATGAGCATGATGAAAGAACTGTATTAATTCTTGTTAATGCACAACAA
    CGTAAAAAAATCGAATCATTATTGAGTCGAGTAAATAAACGAATCACTGAAGCAAACAAC
    GAAATTGAACTATAA
    60
    120
    180
    240
    300
    360
    375

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00620
  • symbol: SAOUHSC_00620
  • description: accessory regulator A
  • length: 124
  • theoretical pI: 8.4094
  • theoretical MW: 14717.9
  • GRAVY: -0.495968

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionsstaphylococcal accessory regulator family (TIGR01889; HMM-score: 118)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesUDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 13.3)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 13.3)
    MetabolismCentral intermediary metabolismAmino sugarsUDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 13.3)
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 13)
    undecaprenyl-phosphate glucose phosphotransferase (TIGR03023; EC 2.7.8.-; HMM-score: 12.2)
    Genetic information processingProtein fateProtein modification and repair[FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 11.3)
  • TheSEED:  
    Regulation and Cell signalingQuorum sensing and biofilm formationBiofilm formation in Staphylococcus Staphylococcal accessory regulator A (SarA) 
  • PFAM:
    HTH (CL0123) HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 20)
    MarR; MarR family (PF01047; HMM-score: 19.7)
    MarR_2; MarR family (PF12802; HMM-score: 14.5)
    PCI; PCI domain (PF01399; HMM-score: 14.4)
    P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 13.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 20
    • Cmax: 0.113
    • Cmax_pos: 20
    • Smax: 0.174
    • Smax_pos: 31
    • Smean: 0.121
    • D: 0.118
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYISENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRKKIESLLSRVNKRITEANNEIEL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 (SigB*) [3] [4] other strains
    SigB* (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  4. 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

J S Blevins, A F Gillaspy, T M Rechtin, B K Hurlburt, M S Smeltzer
The Staphylococcal accessory regulator (sar) represses transcription of the Staphylococcus aureus collagen adhesin gene (cna) in an agr-independent manner.
Mol. Microbiol.: 1999, 33(2);317-26
[PubMed:10411748] [WorldCat.org] (P p)
A Manna, A L Cheung
Characterization of sarR, a modulator of sar expression in Staphylococcus aureus.
Infect. Immun.: 2001, 69(2);885-96
[PubMed:11159982] [WorldCat.org] [DOI] (P p)
B Fournier, A Klier, G Rapoport
The two-component system ArlS-ArlR is a regulator of virulence gene expression in Staphylococcus aureus.
Mol. Microbiol.: 2001, 41(1);247-61
[PubMed:11454217] [WorldCat.org] (P p)
M Bischoff, J M Entenza, P Giachino
Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus.
J. Bacteriol.: 2001, 183(17);5171-9
[PubMed:11489871] [WorldCat.org] (P p)
P M Dunman, E Murphy, S Haney, D Palacios, G Tucker-Kellogg, S Wu, E L Brown, R J Zagursky, D Shlaes, S J Projan
Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci.
J. Bacteriol.: 2001, 183(24);7341-53
[PubMed:11717293] [WorldCat.org] [DOI] (P p)
Jon S Blevins, Karen E Beenken, Mohamed O Elasri, Barry K Hurlburt, Mark S Smeltzer
Strain-dependent differences in the regulatory roles of sarA and agr in Staphylococcus aureus.
Infect. Immun.: 2002, 70(2);470-80
[PubMed:11796572] [WorldCat.org] (P p)
Jaione Valle, Alejandro Toledo-Arana, Carmen Berasain, Jean-Marc Ghigo, Beatriz Amorena, José R Penadés, Iñigo Lasa
SarA and not sigmaB is essential for biofilm development by Staphylococcus aureus.
Mol. Microbiol.: 2003, 48(4);1075-87
[PubMed:12753197] [WorldCat.org] (P p)
Jutta Rossi, Markus Bischoff, Akihito Wada, Brigitte Berger-Bächi
MsrR, a putative cell envelope-associated element involved in Staphylococcus aureus sarA attenuation.
Antimicrob. Agents Chemother.: 2003, 47(8);2558-64
[PubMed:12878519] [WorldCat.org] (P p)
Lindsey Shaw, Ewa Golonka, Jan Potempa, Simon J Foster
The role and regulation of the extracellular proteases of Staphylococcus aureus.
Microbiology (Reading, Engl.): 2004, 150(Pt 1);217-28
[PubMed:14702415] [WorldCat.org] (P p)
Jessica O O'Leary, Mark J Langevin, Christopher T D Price, Jon S Blevins, Mark S Smeltzer, John E Gustafson
Effects of sarA inactivation on the intrinsic multidrug resistance mechanism of Staphylococcus aureus.
FEMS Microbiol. Lett.: 2004, 237(2);297-302
[PubMed:15321676] [WorldCat.org] [DOI] (P p)
Balakrishnan Prithiviraj, Harsh P Bais, Ajay K Jha, Jorge M Vivanco
Staphylococcus aureus pathogenicity on Arabidopsis thaliana is mediated either by a direct effect of salicylic acid on the pathogen or by SA-dependent, NPR1-independent host responses.
Plant J.: 2005, 42(3);417-32
[PubMed:15842626] [WorldCat.org] [DOI] (P p)
María Pilar Trotonda, Adhar C Manna, Ambrose L Cheung, Iñigo Lasa, José R Penadés
SarA positively controls bap-dependent biofilm formation in Staphylococcus aureus.
J. Bacteriol.: 2005, 187(16);5790-8
[PubMed:16077127] [WorldCat.org] [DOI] (P p)
A L Cheung, S J Projan
Cloning and sequencing of sarA of Staphylococcus aureus, a gene required for the expression of agr.
J. Bacteriol.: 1994, 176(13);4168-72
[PubMed:8021198] [WorldCat.org] (P p)
M G Bayer, J H Heinrichs, A L Cheung
The molecular architecture of the sar locus in Staphylococcus aureus.
J. Bacteriol.: 1996, 178(15);4563-70
[PubMed:8755885] [WorldCat.org] (P p)
Y Chien, A L Cheung
Molecular interactions between two global regulators, sar and agr, in Staphylococcus aureus.
J. Biol. Chem.: 1998, 273(5);2645-52
[PubMed:9446568] [WorldCat.org] (P p)