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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00634
  • pan locus tag?: SAUPAN002507000
  • symbol: SAOUHSC_00634
  • pan gene symbol?: mntC
  • synonym:
  • product: ABC transporter substrate-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00634
  • symbol: SAOUHSC_00634
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 624572..625510
  • length: 939
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAAATTAGTACCTTTATTATTAGCCTTATTACTTCTAGTTGCTGCATGTGGTACT
    GGTGGTAAACAAAGCAGTGATAAGTCAAATGGCAAATTAAAAGTAGTAACGACGAATTCA
    ATTTTATATGATATGGCTAAAAATGTTGGTGGAGACAACGTCGATATTCATAGTATTGTA
    CCTGTTGGTCAAGATCCTCATGAATATGAAGTTAAACCTAAAGATATTAAAAAGTTAACT
    GACGCTGACGTTATTTTATACAACGGATTAAATTTAGAGACTGGTAACGGTTGGTTTGAA
    AAAGCCTTAGAACAGGCTGGTAAATCATTAAAAGATAAAAAAGTTATCGCAGTATCAAAA
    GATGTTAAACCTATCTATTTAAACGGTGAAGAAGGCAACAAAGATAAACAAGATCCACAC
    GCATGGTTAAGTTTAGATAACGGTATTAAATACGTAAAAACAATTCAACAAACATTTATC
    GATAACGACAAAAAACATAAAGCAGATTATGAAAAGCAAGGTAACAAATACATTGCTCAA
    TTGGAAAAATTAAATAATGACAGTAAAGACAGTAAAGACAAATTTAATGACATTCCAAAA
    GAACAACGTGCCATGATTACAAGTGAAGGTGCCTTCAAGTACTTCTCAAAACAATACGGT
    ATTACACCAGGTTATATTTGGGAAATTAACACTGAAAAACAAGGTACACCTGAACAAATG
    AGACAAGCTATTGAGTTTGTTAAAAAGCACAAATTAAAACACTTATTAGTAGAAACAAGT
    GTTGATAAGAAAGCAATGGAAAGTTTATCTGAAGAAACGAAGAAAGATATCTTTGGTGAA
    GTGTACACAGATTCAATCGGTAAAGAAGGCACTAAAGGTGACTCTTACTACAAAATGATG
    AAATCAAATATTGAAACTGTACACGGAAGCATGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00634
  • symbol: SAOUHSC_00634
  • description: ABC transporter substrate-binding protein
  • length: 312
  • theoretical pI: 9.15937
  • theoretical MW: 35070.8
  • GRAVY: -0.722756

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsUnknown substrateanchored repeat ABC transporter, substrate-binding protein (TIGR03772; HMM-score: 120)
  • TheSEED:  
    Manganese ABC transporter, periplasmic-binding protein SitA 
  • PFAM:
    Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 235.7)
    RF (CL0337) MRP-S28; Mitochondrial ribosomal subunit protein (PF10213; HMM-score: 18.7)
    GlnB-like (CL0089) Nit_Regul_Hom; Uncharacterized protein, homolog of nitrogen regulatory protein PII (PF10126; HMM-score: 17.2)
    no clan definedeIF3_N; eIF3 subunit 6 N terminal domain (PF09440; HMM-score: 16.1)
    DUF1454; Protein of unknown function (DUF1454) (PF07305; HMM-score: 12.6)
    DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_01683(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    SAOUHSC_00903signal peptidase IB  [1] (data from MRSA252)
    SAOUHSC_02441alkaline shock protein 23  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: -0.33
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LLVAACG
  • SignalP: Signal peptide length 26 aa
    • Ymax: 0.362
    • Ymax_pos: 26
    • Cmax: 0.185
    • Cmax_pos: 26
    • Smax: 0.933
    • Smax_pos: 2
    • Smean: 0.804
    • D: 0.535
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYDMAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKALEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFIDNDKKHKADYEKQGNKYIAQLEKLNNDSKDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGITPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFGEVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_00638 (MntR*)
    MntR* (TF) important in Manganese homeostasis  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2

Relevant publications[edit source | edit]