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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00754
  • pan locus tag?: SAUPAN002645000
  • symbol: SAOUHSC_00754
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00754
  • symbol: SAOUHSC_00754
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 737200..738078
  • length: 879
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAAAAATTGTTATTATCGCTGTTTTAGCGATTTTATTTGTAGTAATAAGTGCTTGT
    GGTAATAAAGAAAAAGAGGCACAACATCAATTTACTAAGCAATTTAAAGATGTTGAGCAA
    AAACAAAAAGAATTACAACATGTCATGGATAATATACATTTGAAAGAAATTGATCATCTA
    AGTAAAACTGATACAACTGATAAAAATAGTAAAGAATTTAAGGCACTACAAGAAGATGTT
    AAAAACCATCTCATACCTAAATTTGAAGCATATTATAAGTCAGCAAAAAATTTGCCTGAT
    GATACAATGAAAGTTAAGAAATTAAAAAAAGAATATATGACGCTTGCAAATGAGAAGAAG
    GATGCGATATATCAATTAAAAAAATTCATAGGTTTATGTAATCAATCTATCAAGTATAAC
    GAAGACATTTTAGATTATACGAAACAATTTGAAAAAAATAGATACAAAGTTGAATCAGAA
    ATTAAATTAGCTGATAATAAAAGTGAAGCAACTAATCTTACGACAAAATTAGAACATAAT
    AATAAAGCGTTAAGAGATACTGCGAAGAAGAACCTAGATGATAGTAAAGAAAATGAAGTA
    AAAGGCGCGATTAAAAATCACATTATGCCAATGATTGAAAAGCAAATTACCGATATTAAC
    CAAACTAATATTAGTGATAAGCATGTTAATAATGCAAGGAAAAACGCAATAGAAATGTAT
    TACAGTCTGCAGAACTATTATAATACACGTATTGAAACAATAAAGGTTAGTGAGAAGTTA
    TCAAAAGTCGATGTAGATAAGTTGCCGAAAAAGGGTATAGATATAACTCACGGCGATAAA
    GCCTTTGAAAAAAAGCTTGAAAAATTAGAAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00754
  • symbol: SAOUHSC_00754
  • description: hypothetical protein
  • length: 292
  • theoretical pI: 9.71132
  • theoretical MW: 34082
  • GRAVY: -0.89726

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    WXG100 family type VII secretion target (TIGR03930; HMM-score: 9.2)
    Cellular processesCellular processesCell divisioncell division protein FtsL (TIGR02209; HMM-score: 7.2)
    phage lysis regulatory protein, LysB family (TIGR03495; HMM-score: 6.8)
    Cellular processesCellular processesSporulation and germinationstage II sporulation protein R (TIGR02837; HMM-score: 4.4)
  • TheSEED:  
    FIG01108064: hypothetical protein 
  • PFAM:
    no clan definedIncA; IncA protein (PF04156; HMM-score: 16.3)
    Ca_hom_mod; Calcium homeostasis modulator (PF14798; HMM-score: 14.4)
    4HB_MCP (CL0457) TarH; Tar ligand binding domain homologue (PF02203; HMM-score: 13.3)
    no clan definedDMPK_coil; DMPK coiled coil domain like (PF08826; HMM-score: 13.3)
    DUF3008; Protein of unknwon function (DUF3008) (PF11450; HMM-score: 12.3)
    Ac76; Orf76 (Ac76) (PF05814; HMM-score: 7.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 4.6
    • Cellwall Score: 2.48
    • Extracellular Score: 2.92
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: VVISACG
  • SignalP: Signal peptide length 28 aa
    • Ymax: 0.49
    • Ymax_pos: 28
    • Cmax: 0.324
    • Cmax_pos: 28
    • Smax: 0.909
    • Smax_pos: 18
    • Smean: 0.766
    • D: 0.597
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKELQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDTMKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESEIKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITDINQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDITHGDKAFEKKLEKLEEK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]