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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00765
  • pan locus tag?: SAUPAN002659000
  • symbol: SAOUHSC_00765
  • pan gene symbol?: comFA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00765
  • symbol: SAOUHSC_00765
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 747288..748226
  • length: 939
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    TTGTTATATGCCGTAACAGGTGCAGGTAAGACAGAAATGATGTTTCAAGGCATTCAATAT
    GCAAGAATACAGGGAGATAATATAGCTATTGTGTCACCACGTGTAGATGTTGTTGTAGAA
    ATTAGTAAACGTATTAAAGACGCATTTCTTAATGAAGATATAGACATACTACACCAGCAA
    TCAAGACAACAATTTGAAGGGCATTTTGTTGTATGCACAGTGCATCAACTTTACCGATTC
    AAACAGCACTTTGATACTATTTTTATTGATGAAGTCGATGCCTTTCCTTTATCAATGGAT
    AAAAATTTACAACAAGCATTGAAGTCATCTTCTAAAGTTGAACATGCAACAATTTATATG
    ACAGCAACACCACCGAAACAACTTCTGTCAGAGATTCCCCACGAAAATATAATTAAATTG
    CCAGCTCGCTTTCATAAAAAATCACTTCCAGTTCCTAAATATCGTTATTTCAAACTTAAT
    AATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGT
    TATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAG
    CAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTT
    GAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAATATTAGAACGTGGA
    TTTACAATGGCAAATTTGGATGTTGTTGTTATCGATGCACATCAATATACTCAAGAGGCT
    TTAATACAAATTGCTGGACGTGTTGGACGAAAATTAGAATGTCCTACTGGAAAAGTATTG
    TTTTTTCATGAAGGGGTAAGTATGAATATGATTCAAGCTAAAAAAGAGATTCAAAGGATG
    AACAAATTAGCATTAAAAAGAGGTTGGATTGATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00765
  • symbol: SAOUHSC_00765
  • description: hypothetical protein
  • length: 312
  • theoretical pI: 9.88503
  • theoretical MW: 36479.2
  • GRAVY: -0.273718

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 51.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 43.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairprimosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 28.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 23.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtranscription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 20.6)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 19.9)
  • TheSEED:  
    DNA MetabolismDNA uptake, competenceGram Positive Competence ComF operon protein A, DNA transporter ATPase 
    DNA uptake, competenceLate competence ComF operon protein A, DNA transporter ATPase 
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 44.4)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 34.1)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 21.5)
    no clan definedPrpR_N; Propionate catabolism activator (PF06506; HMM-score: 19.8)
    P-loop_NTPase (CL0023) Helicase_RecD; Helicase (PF05127; HMM-score: 19)
    Flavi_DEAD; Flavivirus DEAD domain (PF07652; HMM-score: 18.8)
    Glyco_hydro_tim (CL0058) Glyco_hydro_70; Glycosyl hydrolase family 70 (PF02324; HMM-score: 10.8)
    P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 22
    • Cmax: 0.184
    • Cmax_pos: 22
    • Smax: 0.176
    • Smax_pos: 20
    • Smean: 0.082
    • D: 0.111
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDIDILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSKVEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYRILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHFDVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGVSMNMIQAKKEIQRMNKLALKRGWIDE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]