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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0705 [new locus tag: SA_RS04010 ]
  • pan locus tag?: SAUPAN002659000
  • symbol: SA0705
  • pan gene symbol?: comFA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0705 [new locus tag: SA_RS04010 ]
  • symbol: SA0705
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 803795..804697
  • length: 903
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATGTTTCAAGGCATTCAATATGCAAGACGACAGGGAGATAATATAGCTATTGTGTCA
    CCGCGTGTAGATGTTGTTGTAGAAATTAGTAAACGTATTAAAGACGCATTTCTTAATGAA
    GATATAGACATACTACACCAGCAATCAAGTCAACAATTTGAAGGGCATTTTGTTGTATGC
    ACAGTACATCAACTTTACCGATTCAAACAGCACTTTGATACTATTTTTATTGATGAAGTC
    GATGCCTTTCCTTTATCAATGGATAAAAGTTTACAACAAGCATTGAAGTCATCTTCTAAA
    GTTGAACATGCAACGATTTATATGACAGCAACACCACCGAAACAACTTCTGTCAGAGATT
    CCCCACGAAAATATAATTAAATTGCCAGCTCGCTTTCATAAAAAATCACTTCCAGTTCCT
    AAATATCGTTATTTCAAACTTAATAATAATAAGATTCAGAAAATGTTATACCGAATTTTA
    CAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATG
    ATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAG
    GATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTT
    ACTACGACAATATTAGAACGTGGATTTACAATGGCAAATTTGGATGTTGTTGTTATCGAT
    GCACATCAATATACTCAAGAGGCTTTAATACAAATTGCTGGACGTGTTGGACGAAAATTA
    GAATGTCCTGCTGGAAAAGTATTGTTTTTTCATGAAGGAGTAAGTATGAATATGATTCAA
    GCTAAAAAAGAGATTCAAAGGATGAACAAATTAGCATTAAAAAGAGGTTGGATTGATGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0705 [new locus tag: SA_RS04010 ]
  • symbol: SA0705
  • description: hypothetical protein
  • length: 300
  • theoretical pI: 9.84873
  • theoretical MW: 35159.6
  • GRAVY: -0.292333

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 48.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 43.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 23.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtranscription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 20.3)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 20)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairprimosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 10.3)
  • TheSEED:  
    DNA MetabolismDNA uptake, competenceGram Positive Competence ComF operon protein A, DNA transporter ATPase 
    DNA uptake, competenceLate competence ComF operon protein A, DNA transporter ATPase 
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 44.5)
    no clan definedPrpR_N; Propionate catabolism activator (PF06506; HMM-score: 22)
    P-loop_NTPase (CL0023) Flavi_DEAD; Flavivirus DEAD domain (PF07652; HMM-score: 14.5)
    Glyco_hydro_tim (CL0058) Glyco_hydro_70; Glycosyl hydrolase family 70 (PF02324; HMM-score: 11.4)
    P-loop_NTPase (CL0023) Terminase_GpA; Phage terminase large subunit (GpA) (PF05876; HMM-score: 10.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.106
    • Ymax_pos: 39
    • Cmax: 0.111
    • Cmax_pos: 39
    • Smax: 0.143
    • Smax_pos: 33
    • Smean: 0.09
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDIDILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSKVEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYRILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHFDVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPAGKVLFFHEGVSMNMIQAKKEIQRMNKLALKRGWIDE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]