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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00773
  • pan locus tag?: SAUPAN002669000
  • symbol: SAOUHSC_00773
  • pan gene symbol?:
  • synonym:
  • product: LysM domain-containing protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00773
  • symbol: SAOUHSC_00773
  • product: LysM domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 754305..755144
  • length: 840
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAAAATCTCTTACAGTGACGGTTTCGTCAGTGTTAGCTTTTTTAGCTTTAAATAAT
    GCAGCACATGCACAACAACATGGCACACAAGTAAAAACACCTGTTCAACATAATTATGTA
    TCAAATGTTCAAGCACAAACGCAATCACCGACAACTTATACAGTAGTTGCTGGCGATTCA
    TTATATAAGATTGCTTTAGAGCATCACTTAACGTTGAATCAATTATATTCATACAATCCT
    GGTGTAACACCTTTAATTTTTCCTGGTGACGTGATTTCACTTGTGCCTCAAAATAAAGTG
    AAACAAACTAAAGCGGTTAAATCACCAGTAAGAAAAGCAAGCCAAGCTAAAAAGGTAGTA
    AAACAACCTGTACAACAAGCATCTAAAAAAGTAGTAGTTAAGCAAGCACCTAAGCAAGCA
    GTAACTAAGACAGTTAATGTAGCATACAAACCTGCTCAAGTACAAAAATCAGTACCAACT
    GTACCTGTTGCACATAACTACAATAAATCAGTTGCTAACAGAGGAAACTTATATGCTTAT
    GGAAACTGCACATATTATGCTTTCGATCGTCGTGCACAATTAGGTAGAAGTATAGGAAGT
    TTATGGGGCAATGCAAATAACTGGAATTACGCAGCAAAAGTTGCAGGATTTAAAGTAGAT
    AAAACACCAGAAGTTGGCGCTATTTTCCAAACAGCTGCTGGTCCATATGGACATGTTGGT
    GTTGTTGAATCTGTAAACCCTAATGGAACAATTACTGTTTCTGAAATGAACTATGCTGGA
    TTTAATGTTAAATCTTCAAGAACAATTTTAAATCCAGGAAAATATAATTACATCCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00773
  • symbol: SAOUHSC_00773
  • description: LysM domain-containing protein
  • length: 279
  • theoretical pI: 10.557
  • theoretical MW: 30283.3
  • GRAVY: -0.304301

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesSporulation and germinationspore coat assembly protein SafA (TIGR02899; HMM-score: 18.5)
    alternate signal-mediated exported protein, RER_14450 family (TIGR04089; HMM-score: 13.9)
  • TheSEED:  
    LysM domain protein 
  • PFAM:
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 59.7)
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 35.5)
    no clan definedDUF521; Protein of unknown function (DUF521) (PF04412; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: NAAHAQQH
  • SignalP: Signal peptide length 25 aa
    • Ymax: 0.716
    • Ymax_pos: 25
    • Cmax: 0.573
    • Cmax_pos: 25
    • Smax: 0.963
    • Smax_pos: 20
    • Smean: 0.896
    • D: 0.786
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQAQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQTKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPTVPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFKVDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYNYIH

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]